Q86T96 (RN180_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase RNF180
EC=6.3.2.-

Alternative name(s):
RING finger protein 180

Gene names

Name:

RNF180

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase which promotes polyubiquitination and degradation by the proteasome pathway of ZIC2 By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with ZIC2 By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus envelope By similarity.
Domain	The RING-type zinc finger domain mediates polyubiquitination of the interacting protein.
Sequence similarities	Contains 1 RING-type zinc finger.
Sequence caution	The sequence CAD89939.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Endoplasmic reticulum Membrane Nucleus
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: Compara nuclear envelope Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ubiquitin-protein ligase activity Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86T96-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86T96-2)

The sequence of this isoform differs from the canonical sequence as follows:
     410-416: TLNNEMS → VSIYLLI
     417-592: Missing.

Isoform 3 (identifier: Q86T96-3)

The sequence of this isoform differs from the canonical sequence as follows:
     78-397: Missing.
     410-416: TLNNEMS → VSIYLLI
     417-592: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	E3 ubiquitin-protein ligase RNF180		PRO_0000261617
Regions
	Topological domain	1 – 564	564	Cytoplasmic Potential
	Transmembrane	565 – 585	21	Helical; Potential
	Topological domain	586 – 592	7	Extracellular Potential
	Zinc finger	432 – 474	43	RING-type
	Region	281 – 489	209	Interaction with ZIC2 By similarity
Natural variations
	Alternative sequence	78 – 397	320	Missing in isoform 3.		VSP_021738
	Alternative sequence	410 – 416	7	TLNNEMS → VSIYLLI in isoform 2 and isoform 3.		VSP_021739
	Alternative sequence	417 – 592	176	Missing in isoform 2 and isoform 3.		VSP_021740
Experimental info
	Sequence conflict	15	1	E → G in CAD89939. Ref.2
	Sequence conflict	18 – 22	5	SILRC → GETVFSL in CAD89939. Ref.2
	Sequence conflict	458	1	R → G in CAD89939. Ref.2
	Sequence conflict	526	1	G → A in CAD89939. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 28, 2006. Version 2. Checksum: 030AF3FD04ECA57B	FASTA	592	68,254
	10 20 30 40 50 60 MKRSKELITK NHSQEETSIL RCWKCRKCIA SSGCFMEYLE NQVIKDKDDS VDAQNICHVW 70 80 90 100 110 120 HMNVEALPEW ISCLIQKAQW TVGKLNCPFC GARLGGFNFV STPKCSCGQL AAVHLSKSRT 130 140 150 160 170 180 DYQPTQAGRL MRPSVKYLSH PRVQSGCDKE ALLTGGGSEN RNHRLLNMAR NNNDPGRLTE 190 200 210 220 230 240 ALCLEVRPTY FEMKNEKLLS KASEPKYQLF VPQLVTGRCA TRAFHRKSHS LDLNISEKLT 250 260 270 280 290 300 LLPTLYEIHS KTTAYSRLNE TQPIDLSGLP LQSSKNSYSF QNPSSFDPSM LLQRFSVAPH 310 320 330 340 350 360 ETQTQRGGEF QCGLEAASVY SDHTNTNNLT FLMDLPSAGR SMPEASDQEE HLSPLDFLHS 370 380 390 400 410 420 ANFSLGSINQ RLNKRERSKL KNLRRKQRRR ERWLQKQGKY SGVGLLDHMT LNNEMSTDED 430 440 450 460 470 480 NEYAEEKDSY ICAVCLDVYF NPYMCYPCHH IFCEPCLRTL AKDNPSSTPC PLCRTIISRV 490 500 510 520 530 540 FFQTELNNAT KTFFTKEYLK IKQSFQKSNS AKWPLPSCRK AFHLFGGFRR HAAPVTRRQF 550 560 570 580 590 PHGAHRMDYL HFEDDSRGWW FDMDMVIIYI YSVNWVIGFI VFCFLCYFFF PF
	Isoform 2 [UniParc]. Checksum: 9BA191F2271C6A0E	FASTA	416	47,286
	10 20 30 40 50 60 MKRSKELITK NHSQEETSIL RCWKCRKCIA SSGCFMEYLE NQVIKDKDDS VDAQNICHVW 70 80 90 100 110 120 HMNVEALPEW ISCLIQKAQW TVGKLNCPFC GARLGGFNFV STPKCSCGQL AAVHLSKSRT 130 140 150 160 170 180 DYQPTQAGRL MRPSVKYLSH PRVQSGCDKE ALLTGGGSEN RNHRLLNMAR NNNDPGRLTE 190 200 210 220 230 240 ALCLEVRPTY FEMKNEKLLS KASEPKYQLF VPQLVTGRCA TRAFHRKSHS LDLNISEKLT 250 260 270 280 290 300 LLPTLYEIHS KTTAYSRLNE TQPIDLSGLP LQSSKNSYSF QNPSSFDPSM LLQRFSVAPH 310 320 330 340 350 360 ETQTQRGGEF QCGLEAASVY SDHTNTNNLT FLMDLPSAGR SMPEASDQEE HLSPLDFLHS 370 380 390 400 410 ANFSLGSINQ RLNKRERSKL KNLRRKQRRR ERWLQKQGKY SGVGLLDHMV SIYLLI
	Isoform 3 [UniParc]. Checksum: 86BDAC24D6C70469	FASTA	96	11,132
	10 20 30 40 50 60 MKRSKELITK NHSQEETSIL RCWKCRKCIA SSGCFMEYLE NQVIKDKDDS VDAQNICHVW 70 80 90 HMNVEALPEW ISCLIQKGKY SGVGLLDHMV SIYLLI

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Cerebellum.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Spinal cord.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK090756 mRNA. Translation: BAC03514.1. AL832580 mRNA. Translation: CAD89939.1. Different initiation. BC101277 mRNA. Translation: AAI01278.1. BC101278 mRNA. Translation: AAI01279.1. BC101279 mRNA. Translation: AAI01280.1. BC101397 mRNA. Translation: AAI01398.1.
IPI	IPI00184160. IPI00646596. IPI00784453.
RefSeq	NP_001107033.1. NM_001113561.1. NP_848627.1. NM_178532.3.
UniGene	Hs.657843.
3D structure databases
ProteinModelPortal	Q86T96.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000373752.
PTM databases
PhosphoSite	Q86T96.
Polymorphism databases
DMDM	118573800.
Proteomic databases
PaxDb	Q86T96.
PRIDE	Q86T96.
Protocols and materials databases
DNASU	285671.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000296615; ENSP00000296615; ENSG00000164197. ENST00000381081; ENSP00000370471; ENSG00000164197. ENST00000389100; ENSP00000373752; ENSG00000164197.
GeneID	285671.
KEGG	hsa:285671.
UCSC	uc003jth.4. human. uc003jti.3. human. uc010iws.3. human.
Organism-specific databases
CTD	285671.
GeneCards	GC05P063498.
HGNC	HGNC:27752. RNF180.
HPA	HPA006897.
neXtProt	NX_Q86T96.
PharmGKB	PA134980027.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG42632.
HOGENOM	HOG000154158.
HOVERGEN	HBG093907.
InParanoid	Q86T96.
KO	K15708.
OMA	KAFHLFG.
OrthoDB	EOG4Q58QC.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q86T96.
Bgee	Q86T96.
CleanEx	HS_RNF180.
Genevestigator	Q86T96.
GermOnline	ENSG00000164197. Homo sapiens.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR017907. Znf_RING_CS. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RNF180. human.
GenomeRNAi	285671.
NextBio	95695.

Entry information

Entry name

RN180_HUMAN

Accession

Primary (citable) accession number: Q86T96
Secondary accession number(s): Q0JSU3, Q495A8, Q8NBD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 28, 2006
Last modified:	May 1, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.