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Protein

Disheveled-associated activator of morphogenesis 2

Gene

DAAM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Disheveled-associated activator of morphogenesis 2
Gene namesi
Name:DAAM2
Synonyms:KIAA0381
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:18143. DAAM2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27130.

Polymorphism and mutation databases

BioMutaiDAAM2.
DMDMi62906888.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10681068Disheveled-associated activator of morphogenesis 2PRO_0000194909Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1015 – 10151PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ86T65.
MaxQBiQ86T65.
PaxDbiQ86T65.
PeptideAtlasiQ86T65.
PRIDEiQ86T65.

PTM databases

iPTMnetiQ86T65.
PhosphoSiteiQ86T65.

Expressioni

Tissue specificityi

Expressed in most tissues examined.

Gene expression databases

BgeeiQ86T65.
CleanExiHS_DAAM2.
ExpressionAtlasiQ86T65. baseline and differential.
GenevisibleiQ86T65. HS.

Organism-specific databases

HPAiHPA051300.
HPA054630.

Interactioni

Protein-protein interaction databases

BioGridi117049. 15 interactions.
IntActiQ86T65. 1 interaction.
STRINGi9606.ENSP00000274867.

Structurei

3D structure databases

ProteinModelPortaliQ86T65.
SMRiQ86T65. Positions 596-997.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 416377GBD/FH3PROSITE-ProRule annotationAdd
BLAST
Domaini518 – 59477FH1Add
BLAST
Domaini595 – 994400FH2PROSITE-ProRule annotationAdd
BLAST
Domaini1016 – 104833DADPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili434 – 51683Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi518 – 60487Pro-richAdd
BLAST

Domaini

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Belongs to the formin homology family.Curated
Contains 1 DAD (diaphanous autoregulatory) domain.PROSITE-ProRule annotation
Contains 1 FH2 (formin homology 2) domain.PROSITE-ProRule annotation
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1922. Eukaryota.
ENOG410XT5Z. LUCA.
GeneTreeiENSGT00760000118986.
HOGENOMiHOG000237318.
HOVERGENiHBG101333.
InParanoidiQ86T65.
KOiK04512.
OMAiGAPPCFG.
OrthoDBiEOG7B8S37.
PhylomeDBiQ86T65.
TreeFamiTF314602.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR014767. DAD_dom.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR014768. GBD/FH3_dom.
IPR010473. GTPase-bd.
[Graphical view]
PfamiPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTiSM01139. Drf_FH3. 1 hit.
SM01140. Drf_GBD. 1 hit.
SM00498. FH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86T65-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPRKRSHHG LGFLCCFGGS DIPEINLRDN HPLQFMEFSS PIPNAEELNI
60 70 80 90 100
RFAELVDELD LTDKNREAMF ALPPEKKWQI YCSKKKEQED PNKLATSWPD
110 120 130 140 150
YYIDRINSMA AMQSLYAFDE EETEMRNQVV EDLKTALRTQ PMRFVTRFIE
160 170 180 190 200
LEGLTCLLNF LRSMDHATCE SRIHTSLIGC IKALMNNSQG RAHVLAQPEA
210 220 230 240 250
ISTIAQSLRT ENSKTKVAVL EILGAVCLVP GGHKKVLQAM LHYQVYAAER
260 270 280 290 300
TRFQTLLNEL DRSLGRYRDE VNLKTAIMSF INAVLNAGAG EDNLEFRLHL
310 320 330 340 350
RYEFLMLGIQ PVIDKLRQHE NAILDKHLDF FEMVRNEDDL ELARRFDMVH
360 370 380 390 400
IDTKSASQMF ELIHKKLKYT EAYPCLLSVL HHCLQMPYKR NGGYFQQWQL
410 420 430 440 450
LDRILQQIVL QDERGVDPDL APLENFNVKN IVNMLINENE VKQWRDQAEK
460 470 480 490 500
FRKEHMELVS RLERKERECE TKTLEKEEMM RTLNKMKDKL ARESQELRQA
510 520 530 540 550
RGQVAELVAQ LSELSTGPVS SPPPPGGPLT LSSSMTTNDL PPPPPPLPFA
560 570 580 590 600
CCPPPPPPPL PPGGPPTPPG APPCLGMGLP LPQDPYPSSD VPLRKKRVPQ
610 620 630 640 650
PSHPLKSFNW VKLNEERVPG TVWNEIDDMQ VFRILDLEDF EKMFSAYQRH
660 670 680 690 700
QKELGSTEDI YLASRKVKEL SVIDGRRAQN CIILLSKLKL SNEEIRQAIL
710 720 730 740 750
KMDEQEDLAK DMLEQLLKFI PEKSDIDLLE EHKHEIERMA RADRFLYEMS
760 770 780 790 800
RIDHYQQRLQ ALFFKKKFQE RLAEAKPKVE AILLASRELV RSKRLRQMLE
810 820 830 840 850
VILAIGNFMN KGQRGGAYGF RVASLNKIAD TKSSIDRNIS LLHYLIMILE
860 870 880 890 900
KHFPDILNMP SELQHLPEAA KVNLAELEKE VGNLRRGLRA VEVELEYQRR
910 920 930 940 950
QVREPSDKFV PVMSDFITVS SFSFSELEDQ LNEARDKFAK ALMHFGEHDS
960 970 980 990 1000
KMQPDEFFGI FDTFLQAFSE ARQDLEAMRR RKEEEERRAR MEAMLKEQRE
1010 1020 1030 1040 1050
RERWQRQRKV LAAGSSLEEG GEFDDLVSAL RSGEVFDKDL CKLKRSRKRS
1060
GSQALEVTRE RAINRLNY
Length:1,068
Mass (Da):123,499
Last modified:April 26, 2005 - v3
Checksum:i90B071DD1BC13F9A
GO
Isoform 2 (identifier: Q86T65-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     894-894: Missing.

Show »
Length:1,067
Mass (Da):123,370
Checksum:i43C8443B0B176F23
GO

Sequence cautioni

The sequence BAA20835.2 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti414 – 4141R → W in CAD89973 (PubMed:17974005).Curated
Sequence conflicti901 – 9011Q → R in CAD89973 (PubMed:17974005).Curated
Sequence conflicti1003 – 10031R → W in CAD89973 (PubMed:17974005).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti105 – 1051R → H.
Corresponds to variant rs6919807 [ dbSNP | Ensembl ].
VAR_055805
Natural varianti617 – 6171R → H.
Corresponds to variant rs34699846 [ dbSNP | Ensembl ].
VAR_055806

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei894 – 8941Missing in isoform 2. 1 PublicationVSP_047360

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002379 mRNA. Translation: BAA20835.2. Different initiation.
AL833083 mRNA. Translation: CAD89973.1.
AL136089, FO393411 Genomic DNA. Translation: CAI20010.2.
AL161439 Genomic DNA. No translation available.
AL357412 Genomic DNA. No translation available.
AL590999 Genomic DNA. No translation available.
AL592158 Genomic DNA. No translation available.
CH471081 Genomic DNA. Translation: EAX03996.1.
CCDSiCCDS54999.1. [Q86T65-4]
CCDS56426.1. [Q86T65-3]
RefSeqiNP_001188356.1. NM_001201427.1. [Q86T65-3]
NP_056160.2. NM_015345.3. [Q86T65-4]
UniGeneiHs.357128.
Hs.734949.

Genome annotation databases

EnsembliENST00000274867; ENSP00000274867; ENSG00000146122. [Q86T65-3]
ENST00000398904; ENSP00000381876; ENSG00000146122. [Q86T65-3]
ENST00000538976; ENSP00000437808; ENSG00000146122. [Q86T65-4]
GeneIDi23500.
KEGGihsa:23500.
UCSCiuc003oow.4. human. [Q86T65-3]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002379 mRNA. Translation: BAA20835.2. Different initiation.
AL833083 mRNA. Translation: CAD89973.1.
AL136089, FO393411 Genomic DNA. Translation: CAI20010.2.
AL161439 Genomic DNA. No translation available.
AL357412 Genomic DNA. No translation available.
AL590999 Genomic DNA. No translation available.
AL592158 Genomic DNA. No translation available.
CH471081 Genomic DNA. Translation: EAX03996.1.
CCDSiCCDS54999.1. [Q86T65-4]
CCDS56426.1. [Q86T65-3]
RefSeqiNP_001188356.1. NM_001201427.1. [Q86T65-3]
NP_056160.2. NM_015345.3. [Q86T65-4]
UniGeneiHs.357128.
Hs.734949.

3D structure databases

ProteinModelPortaliQ86T65.
SMRiQ86T65. Positions 596-997.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117049. 15 interactions.
IntActiQ86T65. 1 interaction.
STRINGi9606.ENSP00000274867.

PTM databases

iPTMnetiQ86T65.
PhosphoSiteiQ86T65.

Polymorphism and mutation databases

BioMutaiDAAM2.
DMDMi62906888.

Proteomic databases

EPDiQ86T65.
MaxQBiQ86T65.
PaxDbiQ86T65.
PeptideAtlasiQ86T65.
PRIDEiQ86T65.

Protocols and materials databases

DNASUi23500.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000274867; ENSP00000274867; ENSG00000146122. [Q86T65-3]
ENST00000398904; ENSP00000381876; ENSG00000146122. [Q86T65-3]
ENST00000538976; ENSP00000437808; ENSG00000146122. [Q86T65-4]
GeneIDi23500.
KEGGihsa:23500.
UCSCiuc003oow.4. human. [Q86T65-3]

Organism-specific databases

CTDi23500.
GeneCardsiDAAM2.
HGNCiHGNC:18143. DAAM2.
HPAiHPA051300.
HPA054630.
MIMi606627. gene.
neXtProtiNX_Q86T65.
PharmGKBiPA27130.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1922. Eukaryota.
ENOG410XT5Z. LUCA.
GeneTreeiENSGT00760000118986.
HOGENOMiHOG000237318.
HOVERGENiHBG101333.
InParanoidiQ86T65.
KOiK04512.
OMAiGAPPCFG.
OrthoDBiEOG7B8S37.
PhylomeDBiQ86T65.
TreeFamiTF314602.

Miscellaneous databases

ChiTaRSiDAAM2. human.
GenomeRNAii23500.
PROiQ86T65.
SOURCEiSearch...

Gene expression databases

BgeeiQ86T65.
CleanExiHS_DAAM2.
ExpressionAtlasiQ86T65. baseline and differential.
GenevisibleiQ86T65. HS.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR014767. DAD_dom.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR014768. GBD/FH3_dom.
IPR010473. GTPase-bd.
[Graphical view]
PfamiPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTiSM01139. Drf_FH3. 1 hit.
SM01140. Drf_GBD. 1 hit.
SM00498. FH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
    Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. Ohara O., Nagase T., Kikuno R., Nomura N.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Spinal cord.
  4. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1015, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiDAAM2_HUMAN
AccessioniPrimary (citable) accession number: Q86T65
Secondary accession number(s): G5EA45
, Q5T4T8, Q5T4U0, Q9NQI5, Q9Y4G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: April 26, 2005
Last modified: July 6, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.