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Protein

Disheveled-associated activator of morphogenesis 2

Gene

DAAM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key regulator of the Wnt signaling pathway, which is required for various processes during development, such as dorsal patterning, determination of left/right symmetry or myelination in the central nervous system. Acts downstream of Wnt ligands and upstream of beta-catenin (CTNNB1). Required for canonical Wnt signaling pathway during patterning in the dorsal spinal cord by promoting the aggregation of Disheveled (Dvl) complexes, thereby clustering and formation of Wnt receptor signalosomes and potentiating Wnt activity. During dorsal patterning of the spinal cord, inhibits oligodendrocytes differentiation via interaction with PIP5K1A. Also regulates non-canonical Wnt signaling pathway. Acts downstream of PITX2 in the developing gut and is required for left/right asymmetry within dorsal mesentery: affects mesenchymal condensation by lengthening cadherin-based junctions through WNT5A and non-canonical Wnt signaling, inducing polarized condensation in the left dorsal mesentery necessary to initiate gut rotation. Together with DAAM1, required for myocardial maturation and sarcomere assembly.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processWnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Disheveled-associated activator of morphogenesis 2
Gene namesi
Name:DAAM2Imported
Synonyms:KIAA03811 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000146122.16
HGNCiHGNC:18143 DAAM2
MIMi606627 gene
neXtProtiNX_Q86T65

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi23500
OpenTargetsiENSG00000146122
PharmGKBiPA27130

Polymorphism and mutation databases

BioMutaiDAAM2
DMDMi62906888

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949091 – 1068Disheveled-associated activator of morphogenesis 2Add BLAST1068

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1015PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ86T65
PaxDbiQ86T65
PeptideAtlasiQ86T65
PRIDEiQ86T65

PTM databases

iPTMnetiQ86T65
PhosphoSitePlusiQ86T65

Expressioni

Tissue specificityi

Expressed in most tissues examined.1 Publication

Gene expression databases

BgeeiENSG00000146122
CleanExiHS_DAAM2
ExpressionAtlasiQ86T65 baseline and differential
GenevisibleiQ86T65 HS

Organism-specific databases

HPAiHPA051300
HPA054630

Interactioni

Subunit structurei

Interacts with DVL3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi11704917 interactors.
IntActiQ86T65 2 interactors.
STRINGi9606.ENSP00000274867

Structurei

3D structure databases

ProteinModelPortaliQ86T65
SMRiQ86T65
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 416GBD/FH3PROSITE-ProRule annotationAdd BLAST377
Domaini518 – 594FH1Add BLAST77
Domaini595 – 994FH2PROSITE-ProRule annotationAdd BLAST400
Domaini1016 – 1048DADPROSITE-ProRule annotationAdd BLAST33

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili434 – 516Sequence analysisAdd BLAST83

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi518 – 604Pro-richAdd BLAST87

Domaini

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Belongs to the formin homology family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1922 Eukaryota
ENOG410XT5Z LUCA
GeneTreeiENSGT00760000118986
HOGENOMiHOG000237318
HOVERGENiHBG101333
InParanoidiQ86T65
KOiK04512
OrthoDBiEOG091G02QJ
PhylomeDBiQ86T65
TreeFamiTF314602

Family and domain databases

Gene3Di1.25.10.101 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR014767 DAD_dom
IPR015425 FH2_Formin
IPR010472 FH3_dom
IPR014768 GBD/FH3_dom
IPR010473 GTPase-bd
PfamiView protein in Pfam
PF06367 Drf_FH3, 1 hit
PF06371 Drf_GBD, 1 hit
PF02181 FH2, 1 hit
SMARTiView protein in SMART
SM01139 Drf_FH3, 1 hit
SM01140 Drf_GBD, 1 hit
SM00498 FH2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51231 DAD, 1 hit
PS51444 FH2, 1 hit
PS51232 GBD_FH3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86T65-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPRKRSHHG LGFLCCFGGS DIPEINLRDN HPLQFMEFSS PIPNAEELNI
60 70 80 90 100
RFAELVDELD LTDKNREAMF ALPPEKKWQI YCSKKKEQED PNKLATSWPD
110 120 130 140 150
YYIDRINSMA AMQSLYAFDE EETEMRNQVV EDLKTALRTQ PMRFVTRFIE
160 170 180 190 200
LEGLTCLLNF LRSMDHATCE SRIHTSLIGC IKALMNNSQG RAHVLAQPEA
210 220 230 240 250
ISTIAQSLRT ENSKTKVAVL EILGAVCLVP GGHKKVLQAM LHYQVYAAER
260 270 280 290 300
TRFQTLLNEL DRSLGRYRDE VNLKTAIMSF INAVLNAGAG EDNLEFRLHL
310 320 330 340 350
RYEFLMLGIQ PVIDKLRQHE NAILDKHLDF FEMVRNEDDL ELARRFDMVH
360 370 380 390 400
IDTKSASQMF ELIHKKLKYT EAYPCLLSVL HHCLQMPYKR NGGYFQQWQL
410 420 430 440 450
LDRILQQIVL QDERGVDPDL APLENFNVKN IVNMLINENE VKQWRDQAEK
460 470 480 490 500
FRKEHMELVS RLERKERECE TKTLEKEEMM RTLNKMKDKL ARESQELRQA
510 520 530 540 550
RGQVAELVAQ LSELSTGPVS SPPPPGGPLT LSSSMTTNDL PPPPPPLPFA
560 570 580 590 600
CCPPPPPPPL PPGGPPTPPG APPCLGMGLP LPQDPYPSSD VPLRKKRVPQ
610 620 630 640 650
PSHPLKSFNW VKLNEERVPG TVWNEIDDMQ VFRILDLEDF EKMFSAYQRH
660 670 680 690 700
QKELGSTEDI YLASRKVKEL SVIDGRRAQN CIILLSKLKL SNEEIRQAIL
710 720 730 740 750
KMDEQEDLAK DMLEQLLKFI PEKSDIDLLE EHKHEIERMA RADRFLYEMS
760 770 780 790 800
RIDHYQQRLQ ALFFKKKFQE RLAEAKPKVE AILLASRELV RSKRLRQMLE
810 820 830 840 850
VILAIGNFMN KGQRGGAYGF RVASLNKIAD TKSSIDRNIS LLHYLIMILE
860 870 880 890 900
KHFPDILNMP SELQHLPEAA KVNLAELEKE VGNLRRGLRA VEVELEYQRR
910 920 930 940 950
QVREPSDKFV PVMSDFITVS SFSFSELEDQ LNEARDKFAK ALMHFGEHDS
960 970 980 990 1000
KMQPDEFFGI FDTFLQAFSE ARQDLEAMRR RKEEEERRAR MEAMLKEQRE
1010 1020 1030 1040 1050
RERWQRQRKV LAAGSSLEEG GEFDDLVSAL RSGEVFDKDL CKLKRSRKRS
1060
GSQALEVTRE RAINRLNY
Length:1,068
Mass (Da):123,499
Last modified:April 26, 2005 - v3
Checksum:i90B071DD1BC13F9A
GO
Isoform 2 (identifier: Q86T65-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     894-894: Missing.

Show »
Length:1,067
Mass (Da):123,370
Checksum:i43C8443B0B176F23
GO

Sequence cautioni

The sequence BAA20835 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti414R → W in CAD89973 (PubMed:17974005).Curated1
Sequence conflicti901Q → R in CAD89973 (PubMed:17974005).Curated1
Sequence conflicti1003R → W in CAD89973 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055805105R → H. Corresponds to variant dbSNP:rs6919807Ensembl.1
Natural variantiVAR_055806617R → H. Corresponds to variant dbSNP:rs34699846Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047360894Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002379 mRNA Translation: BAA20835.2 Different initiation.
AL833083 mRNA Translation: CAD89973.1
AL136089, FO393411 Genomic DNA Translation: CAI20010.2
AL161439 Genomic DNA No translation available.
AL357412 Genomic DNA No translation available.
AL590999 Genomic DNA No translation available.
AL592158 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03996.1
CCDSiCCDS54999.1 [Q86T65-4]
CCDS56426.1 [Q86T65-3]
RefSeqiNP_001188356.1, NM_001201427.1 [Q86T65-3]
NP_056160.2, NM_015345.3 [Q86T65-4]
UniGeneiHs.357128
Hs.734949

Genome annotation databases

EnsembliENST00000274867; ENSP00000274867; ENSG00000146122 [Q86T65-3]
ENST00000398904; ENSP00000381876; ENSG00000146122 [Q86T65-3]
ENST00000538976; ENSP00000437808; ENSG00000146122 [Q86T65-4]
GeneIDi23500
KEGGihsa:23500
UCSCiuc003oow.4 human [Q86T65-3]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDAAM2_HUMAN
AccessioniPrimary (citable) accession number: Q86T65
Secondary accession number(s): G5EA45
, Q5T4T8, Q5T4U0, Q9NQI5, Q9Y4G0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: April 26, 2005
Last modified: April 25, 2018
This is version 143 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome