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Protein

Transcriptional regulator Kaiso

Gene

ZBTB33

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1 (PubMed:23251453).6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri494 – 516C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri522 – 544C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri550 – 573C2H2-type 3PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • methyl-CpG binding Source: UniProtKB
  • sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

  • intracellular signal transduction Source: ProtInc
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Wnt signaling pathway

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

SignaLinkiQ86T24.
SIGNORiQ86T24.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulator Kaiso
Alternative name(s):
Zinc finger and BTB domain-containing protein 33
Gene namesi
Name:ZBTB33
Synonyms:KAISO, ZNF348
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:16682. ZBTB33.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi552C → R: Abrogates both sequence-specific and methylation-dependent DNA-binding. 1 Publication1

Organism-specific databases

DisGeNETi10009.
OpenTargetsiENSG00000177485.
PharmGKBiPA134928604.

Polymorphism and mutation databases

BioMutaiZBTB33.
DMDMi84029319.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000469881 – 672Transcriptional regulator KaisoAdd BLAST672

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei251PhosphothreonineCombined sources1
Cross-linki449Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki582Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ86T24.
MaxQBiQ86T24.
PaxDbiQ86T24.
PeptideAtlasiQ86T24.
PRIDEiQ86T24.

PTM databases

iPTMnetiQ86T24.
PhosphoSitePlusiQ86T24.

Expressioni

Tissue specificityi

Expressed in vascular endothelium.1 Publication

Inductioni

Induced in vascular endothelium by wounding. This effect is potentiated by prior laminar shear stress, which enhances wound closure.1 Publication

Gene expression databases

BgeeiENSG00000177485.
CleanExiHS_ZBTB33.
GenevisibleiQ86T24. HS.

Organism-specific databases

HPAiCAB001980.
HPA000755.
HPA005732.

Interactioni

Subunit structurei

Self-associates. Interacts with CTNND2 (By similarity). Interacts with CTNND1, and this interaction inhibits binding to both methylated and non-methylated DNA. Interacts with NCOR1. Interacts with KPNA2/RCH1, which may mediate nuclear import of this protein. Interacts with CBFA2T3.By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TRIML2Q8N7C33EBI-2515625,EBI-11059915

Protein-protein interaction databases

BioGridi115327. 49 interactors.
IntActiQ86T24. 25 interactors.
STRINGi9606.ENSP00000314153.

Structurei

Secondary structure

1672
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 27Combined sources14
Turni28 – 31Combined sources4
Beta strandi34 – 38Combined sources5
Beta strandi41 – 45Combined sources5
Helixi47 – 53Combined sources7
Helixi55 – 60Combined sources6
Turni61 – 63Combined sources3
Beta strandi66 – 70Combined sources5
Helixi75 – 85Combined sources11
Helixi95 – 97Combined sources3
Helixi98 – 108Combined sources11
Helixi111 – 114Combined sources4
Beta strandi483 – 488Combined sources6
Beta strandi491 – 496Combined sources6
Turni497 – 499Combined sources3
Beta strandi502 – 505Combined sources4
Helixi506 – 517Combined sources12
Beta strandi525 – 528Combined sources4
Beta strandi530 – 533Combined sources4
Helixi534 – 545Combined sources12
Beta strandi550 – 552Combined sources3
Turni553 – 555Combined sources3
Beta strandi558 – 561Combined sources4
Helixi562 – 573Combined sources12
Beta strandi577 – 581Combined sources5
Beta strandi584 – 586Combined sources3
Helixi598 – 601Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LT7NMR-A472-604[»]
3FKCX-ray1.70A1-114[»]
3M4TX-ray2.05A1-122[»]
3M8VX-ray2.70A1-122[»]
4F6MX-ray2.40A472-604[»]
4F6NX-ray2.80A472-604[»]
ProteinModelPortaliQ86T24.
SMRiQ86T24.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ86T24.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 94BTBPROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 136Self-associationBy similarityAdd BLAST136
Regioni1 – 103Interaction with NCOR11 PublicationAdd BLAST103
Regioni298 – 573Interaction with CBFA2T31 PublicationAdd BLAST276
Regioni454 – 672Interaction with CTNND1By similarityAdd BLAST219
Regioni514 – 638Required for DNA-bindingBy similarityAdd BLAST125

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi471 – 480Nuclear localization signalBy similarity10

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri494 – 516C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri522 – 544C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri550 – 573C2H2-type 3PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00810000125416.
HOGENOMiHOG000230932.
HOVERGENiHBG079575.
InParanoidiQ86T24.
KOiK10507.
OMAiHSQDPSG.
OrthoDBiEOG091G02SP.
PhylomeDBiQ86T24.
TreeFamiTF333100.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q86T24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESRKLISAT DIQYSGSLLN SLNEQRGHGL FCDVTVIVED RKFRAHKNIL
60 70 80 90 100
SASSTYFHQL FSVAGQVVEL SFIRAEIFAE ILNYIYSSKI VRVRSDLLDE
110 120 130 140 150
LIKSGQLLGV KFIAELGVPL SQVKSISGTA QDGNTEPLPP DSGDKNLVIQ
160 170 180 190 200
KSKDEAQDNG ATIMPIITES FSLSAEDYEM KKIIVTDSDD DDDDVIFCSE
210 220 230 240 250
ILPTKETLPS NNTVAQVQSN PGPVAISDVA PSASNNSPPL TNITPTQKLP
260 270 280 290 300
TPVNQATLSQ TQGSEKLLVS SAPTHLTPNI ILLNQTPLST PPNVSSSLPN
310 320 330 340 350
HMPSSINLLV QNQQTPNSAI LTGNKANEEE EEEIIDDDDD TISSSPDSAV
360 370 380 390 400
SNTSLVPQAD TSQNTSFDGS LIQKMQIPTL LQEPLSNSLK ISDIITRNTN
410 420 430 440 450
DPGVGSKHLM EGQKIITLDT ATEIEGLSTG CKVYANIGED TYDIVIPVKD
460 470 480 490 500
DPDEGEARLE NEIPKTSGSE MANKRMKVKH DDHYELIVDG RVYYICIVCK
510 520 530 540 550
RSYVCLTSLR RHFNIHSWEK KYPCRYCEKV FPLAEYRTKH EIHHTGERRY
560 570 580 590 600
QCLACGKSFI NYQFMSSHIK SVHSQDPSGD SKLYRLHPCR SLQIRQYAYL
610 620 630 640 650
SDRSSTIPAM KDDGIGYKVD TGKEPPVGTT TSTQNKPMTW EDIFIQQEND
660 670
SIFKQNVTDG STEFEFIIPE SY
Length:672
Mass (Da):74,484
Last modified:December 20, 2005 - v2
Checksum:i76D94B4051056DB5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40D → Y in CAD91170 (PubMed:17974005).Curated1
Sequence conflicti179E → G in CAD91170 (PubMed:17974005).Curated1
Sequence conflicti189D → DD in CAD91170 (PubMed:17974005).Curated1
Sequence conflicti189D → DD in CAD97963 (PubMed:17974005).Curated1
Sequence conflicti189D → DD in AAH42753 (PubMed:15489334).Curated1
Sequence conflicti362S → Y in CAD91170 (PubMed:17974005).Curated1
Sequence conflicti670E → V in CAD98016 (PubMed:17974005).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK312321 mRNA. Translation: BAG35243.1.
AL833856 mRNA. Translation: CAD38715.1.
AL833604 mRNA. Translation: CAD91170.1.
BX538016 mRNA. Translation: CAD97963.1.
BX538101 mRNA. Translation: CAD98016.1.
AC002086 Genomic DNA. Translation: AAB54087.1.
CH471107 Genomic DNA. Translation: EAX11891.1.
BC042753 mRNA. Translation: AAH42753.1.
CCDSiCCDS14596.1.
RefSeqiNP_001171671.1. NM_001184742.1.
NP_006768.1. NM_006777.3.
UniGeneiHs.143604.

Genome annotation databases

EnsembliENST00000326624; ENSP00000314153; ENSG00000177485.
ENST00000557385; ENSP00000450969; ENSG00000177485.
GeneIDi10009.
KEGGihsa:10009.
UCSCiuc004esn.2. human.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK312321 mRNA. Translation: BAG35243.1.
AL833856 mRNA. Translation: CAD38715.1.
AL833604 mRNA. Translation: CAD91170.1.
BX538016 mRNA. Translation: CAD97963.1.
BX538101 mRNA. Translation: CAD98016.1.
AC002086 Genomic DNA. Translation: AAB54087.1.
CH471107 Genomic DNA. Translation: EAX11891.1.
BC042753 mRNA. Translation: AAH42753.1.
CCDSiCCDS14596.1.
RefSeqiNP_001171671.1. NM_001184742.1.
NP_006768.1. NM_006777.3.
UniGeneiHs.143604.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LT7NMR-A472-604[»]
3FKCX-ray1.70A1-114[»]
3M4TX-ray2.05A1-122[»]
3M8VX-ray2.70A1-122[»]
4F6MX-ray2.40A472-604[»]
4F6NX-ray2.80A472-604[»]
ProteinModelPortaliQ86T24.
SMRiQ86T24.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115327. 49 interactors.
IntActiQ86T24. 25 interactors.
STRINGi9606.ENSP00000314153.

PTM databases

iPTMnetiQ86T24.
PhosphoSitePlusiQ86T24.

Polymorphism and mutation databases

BioMutaiZBTB33.
DMDMi84029319.

Proteomic databases

EPDiQ86T24.
MaxQBiQ86T24.
PaxDbiQ86T24.
PeptideAtlasiQ86T24.
PRIDEiQ86T24.

Protocols and materials databases

DNASUi10009.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000326624; ENSP00000314153; ENSG00000177485.
ENST00000557385; ENSP00000450969; ENSG00000177485.
GeneIDi10009.
KEGGihsa:10009.
UCSCiuc004esn.2. human.

Organism-specific databases

CTDi10009.
DisGeNETi10009.
GeneCardsiZBTB33.
H-InvDBHIX0017022.
HGNCiHGNC:16682. ZBTB33.
HPAiCAB001980.
HPA000755.
HPA005732.
MIMi300329. gene.
neXtProtiNX_Q86T24.
OpenTargetsiENSG00000177485.
PharmGKBiPA134928604.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00810000125416.
HOGENOMiHOG000230932.
HOVERGENiHBG079575.
InParanoidiQ86T24.
KOiK10507.
OMAiHSQDPSG.
OrthoDBiEOG091G02SP.
PhylomeDBiQ86T24.
TreeFamiTF333100.

Enzyme and pathway databases

SignaLinkiQ86T24.
SIGNORiQ86T24.

Miscellaneous databases

ChiTaRSiZBTB33. human.
EvolutionaryTraceiQ86T24.
GeneWikiiZBTB33.
GenomeRNAii10009.
PROiQ86T24.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000177485.
CleanExiHS_ZBTB33.
GenevisibleiQ86T24. HS.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAISO_HUMAN
AccessioniPrimary (citable) accession number: Q86T24
Secondary accession number(s): B2R5U6
, O00319, Q7Z361, Q8IVP6, Q8N3P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 30, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.