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Protein

Putative adhesion G protein-coupled receptor E4P

Gene

ADGRE4P

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein uncertaini

Functioni

May mediate the cellular interaction between myeloid cells and B-cells.By similarity

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • G-protein coupled receptor activity Source: GDB

GO - Biological processi

  • cell surface receptor signaling pathway Source: InterPro
  • G-protein coupled receptor signaling pathway Source: GDB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Ligandi

Calcium

Protein family/group databases

MEROPSiP02.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative adhesion G protein-coupled receptor E4P
Alternative name(s):
EGF-like module receptor 4
EGF-like module-containing mucin-like hormone receptor-like 4
G-protein coupled receptor 127
G-protein coupled receptor PGR16
Gene namesi
Name:ADGRE4PImported
Synonyms:EMR4, EMR4P, GPR127, PGR16
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:19240. ADGRE4P.

Subcellular locationi

Isoform 1 :

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini16 – 191176ExtracellularSequence analysisAdd
BLAST
Transmembranei192 – 21221Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini213 – 22311CytoplasmicSequence analysisAdd
BLAST
Transmembranei224 – 24421Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini245 – 2506ExtracellularSequence analysis
Transmembranei251 – 27121Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini272 – 29928CytoplasmicSequence analysisAdd
BLAST
Transmembranei300 – 32021Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini321 – 33616ExtracellularSequence analysisAdd
BLAST
Transmembranei337 – 35721Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini358 – 38427CytoplasmicSequence analysisAdd
BLAST
Transmembranei385 – 40521Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini406 – 4138ExtracellularSequence analysis
Transmembranei414 – 43421Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini435 – 45723CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • extracellular region Source: UniProtKB-SubCell
  • integral component of membrane Source: GDB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134948805.

Polymorphism and mutation databases

DMDMi44887876.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1414Sequence analysisAdd
BLAST
Chaini15 – 457443Putative adhesion G protein-coupled receptor E4PPRO_0000012877Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi15 ↔ 24PROSITE-ProRule annotation
Disulfide bondi18 ↔ 30PROSITE-ProRule annotation
Glycosylationi26 – 261N-linked (GlcNAc...)Sequence analysis
Disulfide bondi32 ↔ 52PROSITE-ProRule annotation
Disulfide bondi58 ↔ 71PROSITE-ProRule annotation
Disulfide bondi65 ↔ 80PROSITE-ProRule annotation
Disulfide bondi82 ↔ 103PROSITE-ProRule annotation
Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence analysis
Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence analysis
Glycosylationi245 – 2451N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylated.By similarity
Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei173 – 1742CleavageBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ86SQ3.

Interactioni

Subunit structurei

Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ86SQ3.
SMRiQ86SQ3. Positions 18-86, 141-179, 189-439.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 5339EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini54 – 10451EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini138 – 18548GPSPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 GPS domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000294115.
HOVERGENiHBG048917.
InParanoidiQ86SQ3.
PhylomeDBiQ86SQ3.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR017981. GPCR_2-like.
IPR001740. GPCR_2_EMR1-like_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF07645. EGF_CA. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR01128. EMR1HORMONER.
PR00249. GPCRSECRETIN.
SMARTiSM00179. EGF_CA. 1 hit.
SM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS50026. EGF_3. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86SQ3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSRFLLVLL SGASCPPCPK YASCHNSTHC TCEDGFRARS GRTYFHDSSE
60 70 80 90 100
KCEDINECET GLAKCKYKAY CRNKVGGYIC SCLVKYTLFN FLAGIIDYDH
110 120 130 140 150
PDCYENNSQG TTQSNVDIWV SGVKPGFGKQ LPGDKRTKHI CVYWEGSEGG
160 170 180 190 200
WSTEGCSHVH SNGSYTKCKC FHLSSFAVLV ALAPKEDPVL TVITQVGLTI
210 220 230 240 250
SLLCLFLAIL TFLLCRPIQN TSTSLHLELS LCLFLAHLLF LTGINRTEPE
260 270 280 290 300
VLCSIIAGLL HFLYLACFTW MLLEGLHLFL TVRNLKVANY TSTGRFKKRF
310 320 330 340 350
MYPVGYGIPA VIIAVSAIVG PQNYGTFTCW LKLDKGFIWS FMGPVAVIIL
360 370 380 390 400
INLVFYFQVL WILRSKLSSL NKEVSTIQDT RVMTFKAISQ LFILGCSWGL
410 420 430 440 450
GFFMVEEVGK TIGSIIAYSF TIINTLQGVL LFVVHCLLNR QVRLIILSVI

SLVPKSN
Note: No experimental confirmation available.
Length:457
Mass (Da):50,903
Last modified:June 1, 2003 - v1
Checksum:i04A1AF0B89852D34
GO
Isoform 2 (identifier: Q86SQ3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     12-12: G → GLTVLLALPGSEAKNSG
     120-216: VSGVKPGFGK...LAILTFLLCR → ENLRRNGSRE...LSLINLLGIF
     217-457: Missing.

Note: Due to a frameshift mutation in exon 8 would result in a truncated soluble form.
Show »
Length:232
Mass (Da):25,668
Checksum:i20C6989B83478C08
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei12 – 121G → GLTVLLALPGSEAKNSG in isoform 2. 2 PublicationsVSP_057755
Alternative sequencei120 – 21697VSGVK…FLLCR → ENLRRNGSREDFARRATQLI QSVELSIWNASFASPGKGQI SEFDIVYETKRCNETRENAF LEAGNNTMDINCADALKGNL RESTAVALSLINLLGIF in isoform 2. 2 PublicationsVSP_057756Add
BLAST
Alternative sequencei217 – 457241Missing in isoform 2. 2 PublicationsVSP_057757Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY181245 mRNA. Translation: AAO27357.1.
AF489700 mRNA. No translation available.
AY255550 mRNA. Translation: AAO85062.1.
UniGeneiHs.688043.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY181245 mRNA. Translation: AAO27357.1.
AF489700 mRNA. No translation available.
AY255550 mRNA. Translation: AAO85062.1.
UniGeneiHs.688043.

3D structure databases

ProteinModelPortaliQ86SQ3.
SMRiQ86SQ3. Positions 18-86, 141-179, 189-439.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiP02.005.
GPCRDBiSearch...

Polymorphism and mutation databases

DMDMi44887876.

Proteomic databases

PRIDEiQ86SQ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

HGNCiHGNC:19240. ADGRE4P.
neXtProtiNX_Q86SQ3.
PharmGKBiPA134948805.
GenAtlasiSearch...

Phylogenomic databases

HOGENOMiHOG000294115.
HOVERGENiHBG048917.
InParanoidiQ86SQ3.
PhylomeDBiQ86SQ3.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR017981. GPCR_2-like.
IPR001740. GPCR_2_EMR1-like_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF07645. EGF_CA. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR01128. EMR1HORMONER.
PR00249. GPCRSECRETIN.
SMARTiSM00179. EGF_CA. 1 hit.
SM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS50026. EGF_3. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "There exist at least 30 human G-protein-coupled receptors with long Ser/Thr-rich N-termini."
    Fredriksson R., Gloriam D.E.I., Hoeglund P.J., Lagerstroem M.C., Schioeth H.B.
    Biochem. Biophys. Res. Commun. 301:725-734(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Inactivation of the EGF-TM7 receptor EMR4 after the Pan-Homo divergence."
    Hamann J., Kwakkenbos M.J., de Jong E.C., Heus H., Olsen A.S., van Lier R.A.W.
    Eur. J. Immunol. 33:1365-1371(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 262-310.
  4. "Gene structure and transcript analysis of the human and mouse EGF-TM7 molecule, FIRE."
    Caminschi I., Vandenabeele S., Sofi M., McKnight A.J., Ward N., Brodnicki T.C., Toy T., Lahoud M., Maraskovsky E., Shortman K., Wright M.D.
    DNA Seq. 17:8-14(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  5. Cited for: NOMENCLATURE.

Entry informationi

Entry nameiAGRE4_HUMAN
AccessioniPrimary (citable) accession number: Q86SQ3
Secondary accession number(s): Q86SP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 2003
Last modified: July 6, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Could be the product of a pseudogene. A point deletion in exon 8, which introduces a frame shift leading to a premature stop codon. Thus, a protein expressed by this gene would be soluble rather than expressed on the cell surface. This single-nucleotide deletion has been acquired after human-chimpanzee speciation, about five million years ago.2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.