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Protein

Pleckstrin homology-like domain family B member 2

Gene

PHLDB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to be involved in the assembly of the postsynaptic apparatus. May play a role in acetyl-choline receptor (AChR) aggregation in the postsynaptic membrane (By similarity).By similarity1 Publication

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL

GO - Biological processi

  • establishment of protein localization Source: UniProtKB
  • microtubule cytoskeleton organization Source: UniProtKB
  • negative regulation of focal adhesion assembly Source: UniProtKB
  • negative regulation of stress fiber assembly Source: UniProtKB
  • negative regulation of wound healing, spreading of epidermal cells Source: UniProtKB
  • positive regulation of basement membrane assembly involved in embryonic body morphogenesis Source: UniProtKB
  • regulation of epithelial to mesenchymal transition Source: UniProtKB
  • regulation of gastrulation Source: UniProtKB
  • regulation of microtubule cytoskeleton organization Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:G66-33477-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology-like domain family B member 2
Alternative name(s):
Protein LL5-beta
Gene namesi
Name:PHLDB2
Synonyms:LL5B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:29573. PHLDB2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Membrane 1 Publication; Peripheral membrane protein 1 Publication

  • Note: Translocates to the plasma membrane at high levels of PtdIns(3,4,5)P3. At low levels of PtdIns(3,4,5)P3 is translocated to vesicular compartments.

GO - Cellular componenti

  • basal cortex Source: UniProtKB
  • cell-cell adherens junction Source: BHF-UCL
  • cell leading edge Source: UniProtKB
  • cytoplasm Source: HPA
  • intermediate filament cytoskeleton Source: HPA
  • plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1162 – 1163KR → AA: Loss of binding to PtdIns(3,4,5)P3. 1 Publication2

Organism-specific databases

DisGeNETi90102.
OpenTargetsiENSG00000144824.
PharmGKBiPA134884060.

Polymorphism and mutation databases

BioMutaiPHLDB2.
DMDMi84029396.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538941 – 1253Pleckstrin homology-like domain family B member 2Add BLAST1253

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei71PhosphoserineCombined sources1
Modified residuei73PhosphoserineCombined sources1
Modified residuei157PhosphoserineCombined sources1
Modified residuei204PhosphoserineBy similarity1
Modified residuei212PhosphoserineCombined sources1
Modified residuei242PhosphoserineBy similarity1
Modified residuei245PhosphoserineBy similarity1
Modified residuei330PhosphoserineBy similarity1
Modified residuei334PhosphoserineCombined sources1
Modified residuei348PhosphoserineBy similarity1
Modified residuei351PhosphoserineCombined sources1
Modified residuei384PhosphoserineCombined sources1
Modified residuei387PhosphoserineCombined sources1
Modified residuei415PhosphoserineCombined sources1
Modified residuei420PhosphoserineCombined sources1
Modified residuei468PhosphoserineCombined sources1
Modified residuei489PhosphoserineCombined sources1
Modified residuei501PhosphoserineCombined sources1
Modified residuei504PhosphothreonineCombined sources1
Modified residuei513PhosphoserineCombined sources1
Modified residuei550PhosphothreonineCombined sources1
Modified residuei574PhosphothreonineCombined sources1
Modified residuei898PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ86SQ0.
MaxQBiQ86SQ0.
PaxDbiQ86SQ0.
PeptideAtlasiQ86SQ0.
PRIDEiQ86SQ0.

PTM databases

iPTMnetiQ86SQ0.
PhosphoSitePlusiQ86SQ0.

Expressioni

Gene expression databases

BgeeiENSG00000144824.
CleanExiHS_PHLDB2.
ExpressionAtlasiQ86SQ0. baseline and differential.
GenevisibleiQ86SQ0. HS.

Organism-specific databases

HPAiHPA035146.
HPA035147.

Interactioni

Subunit structurei

Interacts with FLNC.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
FLNAP213333EBI-2798483,EBI-350432

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL

Protein-protein interaction databases

BioGridi124661. 38 interactors.
DIPiDIP-42196N.
IntActiQ86SQ0. 38 interactors.
MINTiMINT-5289240.
STRINGi9606.ENSP00000377502.

Structurei

3D structure databases

ProteinModelPortaliQ86SQ0.
SMRiQ86SQ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1143 – 1246PHPROSITE-ProRule annotationAdd BLAST104

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili584 – 696Sequence analysisAdd BLAST113
Coiled coili722 – 807Sequence analysisAdd BLAST86
Coiled coili1032 – 1098Sequence analysisAdd BLAST67

Domaini

The PH domain mediates the binding to phosphoinositides.

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFMW. Eukaryota.
ENOG4111JWX. LUCA.
GeneTreeiENSGT00530000063148.
HOVERGENiHBG082124.
InParanoidiQ86SQ0.
OMAiYLNSFHY.
OrthoDBiEOG091G0PU7.
PhylomeDBiQ86SQ0.
TreeFamiTF329165.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86SQ0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEHSYIQKE LDLQNGSLEE DSVVHSVEND SQNMMESLSP KKYSSSLRFK
60 70 80 90 100
ANGDYSGSYL TLSQPVPAKR SPSPLGTSVR SSPSLAKIQG SKQFSYDGTD
110 120 130 140 150
KNIPMKPPTP LLNTTSSLSG YPLGRADFDH YTGRDSERAL RLSEKPPYSK
160 170 180 190 200
YSSRHKSHDN VYSLGGLEGR KASGSLLAMW NGSSLSDAGP PPISRSGAAS
210 220 230 240 250
MPSSPKQARK MSIQDSLALQ PKLTRHKELA SENINLRTRK YSSSSLSHMG
260 270 280 290 300
AYSRSLPRLY RATENQLTPL SLPPRNSLGN SKRTKLGEKD LPHSVIDNDN
310 320 330 340 350
YLNFSSLSSG ALPYKTSASE GNPYVSSTLS VPASPRVARK MLLASTSSCA
360 370 380 390 400
SDDFDQASYV GTNPSHSLLA GESDRVFATR RNFSCGSVEF DEADLESLRQ
410 420 430 440 450
ASGTPQPALR ERKSSISSIS GRDDLMDYHR RQREERLREQ EMERLERQRL
460 470 480 490 500
ETILSLCAEY TKPDSRLSTG TTVEDVQKIN KELEKLQLSD EESVFEEALM
510 520 530 540 550
SPDTRYRCHR KDSLPDADLA SCGSLSQSSA SFFTPRSTRN DELLSDLTRT
560 570 580 590 600
PPPPSSTFPK ASSESSYLSI LPKTPEGISE EQRSQELAAM EETRIVILNN
610 620 630 640 650
LEELKQKIKD INDQMDESFR ELDMECALLD GEQKSETTEL MKEKEILDHL
660 670 680 690 700
NRKIAELEKN IVGEKTKEKV KLDAEREKLE RLQELYSEQK TQLDNCPESM
710 720 730 740 750
REQLQQQLKR DADLLDVESK HFEDLEFQQL EHESRLDEEK ENLTQQLLRE
760 770 780 790 800
VAEYQRNIVS RKEKISALKK QANHIVQQAQ REQDHFVKEK NNLIMMLQRE
810 820 830 840 850
KENLCNLEKK YSSLSGGKGF PVNPNTLKEG YISVNEINEP CGNSTNLSPS
860 870 880 890 900
TQFPADADAV ATEPATAVLA SQPQSKEHFR SLEERKKQHK EGLYLSDTLP
910 920 930 940 950
RKKTTSSISP HFSSATMGRS ITPKAHLPLG QSNSCGSVLP PSLAAMAKDS
960 970 980 990 1000
ESRRMLRGYN HQQMSEGHRQ KSEFYNRTAS ESNVYLNSFH YPDHSYKDQA
1010 1020 1030 1040 1050
FDTLSLDSSD SMETSISACS PDNISSASTS NIARIEEMER LLKQAHAEKT
1060 1070 1080 1090 1100
RLLESREREM EAKKRALEEE KRRREILEKR LQEETSQRQK LIEKEVKIRE
1110 1120 1130 1140 1150
RQRAQARPLT RYLPVRKEDF DLRSHVETAG HNIDTCYHVS ITEKTCRGFL
1160 1170 1180 1190 1200
IKMGGKIKTW KKRWFVFDRN KRTFSYYADK HETKLKGVIY FQAIEEVYYD
1210 1220 1230 1240 1250
HLKNANKSPN PLLTFSVKTH DRIYYMVAPS PEAMRIWMDV IVTGAEGYTH

FLL
Length:1,253
Mass (Da):142,158
Last modified:December 20, 2005 - v2
Checksum:iD0950E96AEE1B020
GO
Isoform 2 (identifier: Q86SQ0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     668-710: Missing.

Show »
Length:1,210
Mass (Da):136,886
Checksum:iD05881C68D0A811C
GO
Isoform 3 (identifier: Q86SQ0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MEEEDTKREVPKEDGVGDVQHFDSSKIM
     668-710: Missing.

Show »
Length:1,237
Mass (Da):139,974
Checksum:iBF9C4AF0F1DEC613
GO

Sequence cautioni

The sequence AAH69194 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAD93140 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96Y → H in BAC04014 (PubMed:14702039).Curated1
Sequence conflicti346T → A in CAH18447 (PubMed:17974005).Curated1
Sequence conflicti585Q → E in CAD42711 (PubMed:12376540).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_024760941P → S.1 PublicationCorresponds to variant rs3749298dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0167441M → MEEEDTKREVPKEDGVGDVQ HFDSSKIM in isoform 3. 1 Publication1
Alternative sequenceiVSP_016745668 – 710Missing in isoform 2 and isoform 3. 4 PublicationsAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ496194 mRNA. Translation: CAD42711.1.
AF506820 mRNA. Translation: AAM33634.1.
AB209903 mRNA. Translation: BAD93140.1. Different initiation.
CR749654 mRNA. Translation: CAH18447.1.
CH471052 Genomic DNA. Translation: EAW79690.1.
BC069194 mRNA. Translation: AAH69194.1. Sequence problems.
BC142678 mRNA. Translation: AAI42679.1.
BC150210 mRNA. Translation: AAI50211.1.
AK092996 mRNA. Translation: BAC04014.1.
AK096151 mRNA. Translation: BAC04713.1.
CCDSiCCDS2962.1. [Q86SQ0-2]
CCDS46885.1. [Q86SQ0-3]
CCDS46886.1. [Q86SQ0-1]
RefSeqiNP_001127909.1. NM_001134437.1. [Q86SQ0-3]
NP_001127910.1. NM_001134438.1. [Q86SQ0-1]
NP_001127911.1. NM_001134439.1. [Q86SQ0-1]
NP_665696.1. NM_145753.2. [Q86SQ0-2]
UniGeneiHs.603252.
Hs.679140.

Genome annotation databases

EnsembliENST00000393923; ENSP00000377500; ENSG00000144824. [Q86SQ0-3]
ENST00000393925; ENSP00000377502; ENSG00000144824. [Q86SQ0-1]
ENST00000412622; ENSP00000405292; ENSG00000144824. [Q86SQ0-2]
ENST00000431670; ENSP00000405405; ENSG00000144824. [Q86SQ0-1]
ENST00000481953; ENSP00000418319; ENSG00000144824. [Q86SQ0-2]
GeneIDi90102.
KEGGihsa:90102.
UCSCiuc003dyc.4. human. [Q86SQ0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ496194 mRNA. Translation: CAD42711.1.
AF506820 mRNA. Translation: AAM33634.1.
AB209903 mRNA. Translation: BAD93140.1. Different initiation.
CR749654 mRNA. Translation: CAH18447.1.
CH471052 Genomic DNA. Translation: EAW79690.1.
BC069194 mRNA. Translation: AAH69194.1. Sequence problems.
BC142678 mRNA. Translation: AAI42679.1.
BC150210 mRNA. Translation: AAI50211.1.
AK092996 mRNA. Translation: BAC04014.1.
AK096151 mRNA. Translation: BAC04713.1.
CCDSiCCDS2962.1. [Q86SQ0-2]
CCDS46885.1. [Q86SQ0-3]
CCDS46886.1. [Q86SQ0-1]
RefSeqiNP_001127909.1. NM_001134437.1. [Q86SQ0-3]
NP_001127910.1. NM_001134438.1. [Q86SQ0-1]
NP_001127911.1. NM_001134439.1. [Q86SQ0-1]
NP_665696.1. NM_145753.2. [Q86SQ0-2]
UniGeneiHs.603252.
Hs.679140.

3D structure databases

ProteinModelPortaliQ86SQ0.
SMRiQ86SQ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124661. 38 interactors.
DIPiDIP-42196N.
IntActiQ86SQ0. 38 interactors.
MINTiMINT-5289240.
STRINGi9606.ENSP00000377502.

PTM databases

iPTMnetiQ86SQ0.
PhosphoSitePlusiQ86SQ0.

Polymorphism and mutation databases

BioMutaiPHLDB2.
DMDMi84029396.

Proteomic databases

EPDiQ86SQ0.
MaxQBiQ86SQ0.
PaxDbiQ86SQ0.
PeptideAtlasiQ86SQ0.
PRIDEiQ86SQ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000393923; ENSP00000377500; ENSG00000144824. [Q86SQ0-3]
ENST00000393925; ENSP00000377502; ENSG00000144824. [Q86SQ0-1]
ENST00000412622; ENSP00000405292; ENSG00000144824. [Q86SQ0-2]
ENST00000431670; ENSP00000405405; ENSG00000144824. [Q86SQ0-1]
ENST00000481953; ENSP00000418319; ENSG00000144824. [Q86SQ0-2]
GeneIDi90102.
KEGGihsa:90102.
UCSCiuc003dyc.4. human. [Q86SQ0-1]

Organism-specific databases

CTDi90102.
DisGeNETi90102.
GeneCardsiPHLDB2.
HGNCiHGNC:29573. PHLDB2.
HPAiHPA035146.
HPA035147.
MIMi610298. gene.
neXtProtiNX_Q86SQ0.
OpenTargetsiENSG00000144824.
PharmGKBiPA134884060.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFMW. Eukaryota.
ENOG4111JWX. LUCA.
GeneTreeiENSGT00530000063148.
HOVERGENiHBG082124.
InParanoidiQ86SQ0.
OMAiYLNSFHY.
OrthoDBiEOG091G0PU7.
PhylomeDBiQ86SQ0.
TreeFamiTF329165.

Enzyme and pathway databases

BioCyciZFISH:G66-33477-MONOMER.

Miscellaneous databases

ChiTaRSiPHLDB2. human.
GeneWikiiPHLDB2.
GenomeRNAii90102.
PROiQ86SQ0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000144824.
CleanExiHS_PHLDB2.
ExpressionAtlasiQ86SQ0. baseline and differential.
GenevisibleiQ86SQ0. HS.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHLB2_HUMAN
AccessioniPrimary (citable) accession number: Q86SQ0
Secondary accession number(s): A5PKZ3
, Q59EA8, Q68CY3, Q6NT98, Q8N8U8, Q8NAB1, Q8NCU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.