Q86SJ2 (AMGO2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amphoterin-induced protein 2

Alternative name(s):
AMIGO-2
Alivin-1
Differentially expressed in gastric adenocarcinomas
Short name=DEGA

Gene names

Name:	AMIGO2
Synonyms:	ALI1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas. Ref.3 UniProtKB Q7TNJ4
Subunit structure	Binds itself as well as AMIGO1 and AMIGO3 By similarity.
Subcellular location	Cell membrane; Single-pass type I membrane protein By similarity. Nucleus By similarity. Note: Associated with nucleus as well as plasma membrane. Restricted to somata of cerebellar as well as hippocampal neurons By similarity.
Tissue specificity	Highest levels in breast, ovary, cervix, and uterus. Lower levels in lung, colon, and rectum. Differentially expressed in 56% of thyroid, 57% of pancreatic and 45% of stomach cancers. Ref.3
Sequence similarities	Belongs to the immunoglobulin superfamily. AMIGO family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 6 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane Nucleus
Domain	Immunoglobulin domain Leucine-rich repeat Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heterophilic cell-cell adhesion Inferred from sequence or structural similarity Ref.1. Source: UniProtKB homophilic cell adhesion Inferred from sequence or structural similarity Ref.1. Source: UniProtKB negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of programmed cell death Inferred from sequence or structural similarity Ref.2. Source: UniProtKB
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CACNA1A	O00555	2	EBI-3866830,EBI-766279

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 39	39	Potential
	Chain	40 – 522	483	Amphoterin-induced protein 2		PRO_0000014509
Regions
	Topological domain	40 – 398	359	Extracellular Potential
	Transmembrane	399 – 419	21	Helical; Potential
	Topological domain	420 – 522	103	Cytoplasmic Potential
	Domain	40 – 68	29	LRRNT
	Repeat	69 – 90	22	LRR 1
	Repeat	94 – 115	22	LRR 2
	Repeat	118 – 139	22	LRR 3
	Repeat	142 – 163	22	LRR 4
	Repeat	166 – 187	22	LRR 5
	Repeat	193 – 214	22	LRR 6
	Domain	228 – 284	57	LRRCT
	Domain	289 – 379	91	Ig-like C2-type
Amino acid modifications
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
	Glycosylation	281	1	N-linked (GlcNAc...) Potential
	Glycosylation	288	1	N-linked (GlcNAc...) Potential
	Glycosylation	345	1	N-linked (GlcNAc...) Potential
	Glycosylation	373	1	N-linked (GlcNAc...) Potential
	Glycosylation	381	1	N-linked (GlcNAc...) Potential
	Glycosylation	384	1	N-linked (GlcNAc...) Potential
	Disulfide bond	41 ↔ 47		By similarity
	Disulfide bond	45 ↔ 54		By similarity
	Disulfide bond	232 ↔ 260		By similarity
	Disulfide bond	234 ↔ 282		By similarity
	Disulfide bond	310 ↔ 363		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q86SJ2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 20A86E3D82C05B9B	FASTA	522	57,934
	10 20 30 40 50 60 MSLRVHTLPT LLGAVVRPGC RELLCLLMIT VTVGPGASGV CPTACICATD IVSCTNKNLS 70 80 90 100 110 120 KVPGNLFRLI KRLDLSYNRI GLLDSEWIPV SFAKLNTLIL RHNNITSIST GSFSTTPNLK 130 140 150 160 170 180 CLDLSSNKLK TVKNAVFQEL KVLEVLLLYN NHISYLDPSA FGGLSQLQKL YLSGNFLTQF 190 200 210 220 230 240 PMDLYVGRFK LAELMFLDVS YNRIPSMPMH HINLVPGKQL RGIYLHGNPF VCDCSLYSLL 250 260 270 280 290 300 VFWYRRHFSS VMDFKNDYTC RLWSDSRHSR QVLLLQDSFM NCSDSIINGS FRALGFIHEA 310 320 330 340 350 360 QVGERLMVHC DSKTGNANTD FIWVGPDNRL LEPDKEMENF YVFHNGSLVI ESPRFEDAGV 370 380 390 400 410 420 YSCIAMNKQR LLNETVDVTI NVSNFTVSRS HAHEAFNTAF TTLAACVASI VLVLLYLYLT 430 440 450 460 470 480 PCPCKCKTKR QKNMLHQSNA HSSILSPGPA SDASADERKA GAGKRVVFLE PLKDTAAGQN 490 500 510 520 GKVRLFPSEA VIAEGILKST RGKSDSDSVN SVFSDTPFVA ST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AMIGO, a transmembrane protein implicated in axon tract development, defines a novel protein family with leucine-rich repeats." Kuja-Panula J., Kiiltomaeki M., Yamashiro T., Rouhiainen A., Rauvala H. J. Cell Biol. 160:963-973(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fibrosarcoma.
[2]	"Alivin 1, a novel neuronal activity-dependent gene, inhibits apoptosis and promotes survival of cerebellar granule neurons." Ono T., Sekino-Suzuki N., Kikkawa Y., Yonekawa H., Kawashima S. J. Neurosci. 23:5887-5896(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"DEGA/AMIGO-2, a leucine-rich repeat family member, differentially expressed in human gastric adenocarcinoma: effects on ploidy, chromosomal stability, cell adhesion/migration and tumorigenicity." Rabenau K.E., O'Toole J.M., Bassi R., Kotanides H., Witte L., Ludwig D.L., Pereira D.S. Oncogene 23:5056-5067(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Stomach.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis and Uterus.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY237005 mRNA. Translation: AAO48946.1. AB079074 mRNA. Translation: BAC81188.1. AB080610 mRNA. Translation: BAC81189.1. AY454159 mRNA. Translation: AAR83271.1. AL833007 mRNA. Translation: CAH56293.1. BC014103 mRNA. Translation: AAH14103.2. BC047595 mRNA. Translation: AAH47595.1. BC095477 mRNA. Translation: AAH95477.1.
IPI	IPI00238262.
RefSeq	NP_001137140.1. NM_001143668.1. NP_862830.1. NM_181847.4.
UniGene	Hs.121520.
3D structure databases
ProteinModelPortal	Q86SJ2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86SJ2. 1 interaction.
STRING	9606.ENSP00000266581.
Polymorphism databases
DMDM	68052338.
Proteomic databases
PaxDb	Q86SJ2.
PeptideAtlas	Q86SJ2.
PRIDE	Q86SJ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000266581; ENSP00000266581; ENSG00000139211. ENST00000321382; ENSP00000320848; ENSG00000139211. ENST00000429635; ENSP00000406020; ENSG00000139211. ENST00000550413; ENSP00000449034; ENSG00000139211.
GeneID	347902.
KEGG	hsa:347902.
UCSC	uc001rpk.3. human.
Organism-specific databases
CTD	347902.
GeneCards	GC12M047469.
HGNC	HGNC:24073. AMIGO2.
HPA	HPA054004.
neXtProt	NX_Q86SJ2.
PharmGKB	PA142672626.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG146733.
HOGENOM	HOG000231327.
HOVERGEN	HBG080231.
InParanoid	Q86SJ2.
OMA	SCIAMNR.
OrthoDB	EOG43XV36.
PhylomeDB	Q86SJ2.
Gene expression databases
ArrayExpress	Q86SJ2.
Bgee	Q86SJ2.
CleanEx	HS_AMIGO2.
Genevestigator	Q86SJ2.
GermOnline	ENSG00000139211. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.10. 1 hit.
InterPro	IPR000483. Cys-rich_flank_reg_C. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR001611. Leu-rich_rpt. IPR003591. Leu-rich_rpt_typical-subtyp. IPR000372. LRR-contain_N. [Graphical view]
Pfam	PF00560. LRR_1. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. SM00369. LRR_TYP. 1 hit. SM00082. LRRCT. 1 hit. SM00013. LRRNT. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. PS51450. LRR. 7 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	347902.
NextBio	99311.

Entry information

Entry name

AMGO2_HUMAN

Accession

Primary (citable) accession number: Q86SJ2
Secondary accession number(s): Q4VBP6, Q7Z4A0, Q96CN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.