Reviewed, UniProtKB/Swiss-Prot Q86SJ2 (AMGO2_HUMAN)

Names and origin

Protein names

Recommended name:
    Amphoterin-induced protein 2
Alternative name(s):
    AMIGO-2
    Alivin-1
    Differentially expressed in gastric adenocarcinomas
      Short name=DEGA

Gene names

Name:	AMIGO2
Synonyms:	ALI1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas. Ref.3 UniProtKB Q7TNJ4
Subunit structure	Binds itself as well as AMIGO1 and AMIGO3 By similarity.
Subcellular location	Cell membrane; Single-pass type I membrane protein By similarity. Nucleus By similarity. Note: Associated with nucleus as well as plasma membrane. Restricted to somata of cerebellar as well as hippocampal neurons By similarity.
Tissue specificity	Highest levels in breast, ovary, cervix, and uterus. Lower levels in lung, colon, and rectum. Differentially expressed in 56% of thyroid, 57% of pancreatic and 45% of stomach cancers. Ref.3
Sequence similarities	Belongs to the immunoglobulin superfamily. AMIGO family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 6 LRR (leucine-rich) repeats.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane Nucleus
Domain	Immunoglobulin domain Leucine-rich repeat Repeat Signal Transmembrane
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heterophilic cell adhesion Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB homophilic cell adhesion Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 39	39	Potential
	Chain	40 – 522	483	Amphoterin-induced protein 2		PRO_0000014509
Regions
	Topological domain	40 – 398	359	Extracellular Potential
	Transmembrane	399 – 419	21	Potential
	Topological domain	420 – 522	103	Cytoplasmic Potential
	Repeat	67 – 90	24	LRR 1
	Repeat	92 – 115	24	LRR 2
	Repeat	116 – 139	24	LRR 3
	Repeat	141 – 163	23	LRR 4
	Repeat	164 – 187	24	LRR 5
	Repeat	191 – 214	24	LRR 6
	Domain	289 – 379	91	Ig-like C2-type
Amino acid modifications
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
	Glycosylation	281	1	N-linked (GlcNAc...) Potential
	Glycosylation	288	1	N-linked (GlcNAc...) Potential
	Glycosylation	345	1	N-linked (GlcNAc...) Potential
	Glycosylation	373	1	N-linked (GlcNAc...) Potential
	Glycosylation	381	1	N-linked (GlcNAc...) Potential
	Glycosylation	384	1	N-linked (GlcNAc...) Potential
	Disulfide bond	310 ↔ 363		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q86SJ2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 20A86E3D82C05B9B	FASTA	522	57,934
	10 20 30 40 50 60 MSLRVHTLPT LLGAVVRPGC RELLCLLMIT VTVGPGASGV CPTACICATD IVSCTNKNLS 70 80 90 100 110 120 KVPGNLFRLI KRLDLSYNRI GLLDSEWIPV SFAKLNTLIL RHNNITSIST GSFSTTPNLK 130 140 150 160 170 180 CLDLSSNKLK TVKNAVFQEL KVLEVLLLYN NHISYLDPSA FGGLSQLQKL YLSGNFLTQF 190 200 210 220 230 240 PMDLYVGRFK LAELMFLDVS YNRIPSMPMH HINLVPGKQL RGIYLHGNPF VCDCSLYSLL 250 260 270 280 290 300 VFWYRRHFSS VMDFKNDYTC RLWSDSRHSR QVLLLQDSFM NCSDSIINGS FRALGFIHEA 310 320 330 340 350 360 QVGERLMVHC DSKTGNANTD FIWVGPDNRL LEPDKEMENF YVFHNGSLVI ESPRFEDAGV 370 380 390 400 410 420 YSCIAMNKQR LLNETVDVTI NVSNFTVSRS HAHEAFNTAF TTLAACVASI VLVLLYLYLT 430 440 450 460 470 480 PCPCKCKTKR QKNMLHQSNA HSSILSPGPA SDASADERKA GAGKRVVFLE PLKDTAAGQN 490 500 510 520 GKVRLFPSEA VIAEGILKST RGKSDSDSVN SVFSDTPFVA ST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AMIGO, a transmembrane protein implicated in axon tract development, defines a novel protein family with leucine-rich repeats." Kuja-Panula J., Kiiltomaeki M., Yamashiro T., Rouhiainen A., Rauvala H. J. Cell Biol. 160:963-973(2003) [PubMed: 12629050] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fibrosarcoma.
[2]	"Alivin 1, a novel neuronal activity-dependent gene, inhibits apoptosis and promotes survival of cerebellar granule neurons." Ono T., Sekino-Suzuki N., Kikkawa Y., Yonekawa H., Kawashima S. J. Neurosci. 23:5887-5896(2003) [PubMed: 12843293] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"DEGA/AMIGO-2, a leucine-rich repeat family member, differentially expressed in human gastric adenocarcinoma: effects on ploidy, chromosomal stability, cell adhesion/migration and tumorigenicity." Rabenau K.E., O'Toole J.M., Bassi R., Kotanides H., Witte L., Ludwig D.L., Pereira D.S. Oncogene 23:5056-5067(2004) [PubMed: 15107827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Stomach.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis and Uterus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY237005 mRNA. Translation: AAO48946.1. AB079074 mRNA. Translation: BAC81188.1. AB080610 mRNA. Translation: BAC81189.1. AY454159 mRNA. Translation: AAR83271.1. AL833007 mRNA. Translation: CAH56293.1. BC014103 mRNA. Translation: AAH14103.2. BC047595 mRNA. Translation: AAH47595.1. BC095477 mRNA. Translation: AAH95477.1.
IPI	IPI00238262.
RefSeq	NP_001137140.1. NP_862830.1.
UniGene	Hs.121520
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q86SJ2.
Proteomic databases
PeptideAtlas	Q86SJ2.
PRIDE	Q86SJ2.
Genome annotation databases
Ensembl	ENST00000266581; ENSP00000266581; ENSG00000139211; Homo sapiens. [Genome view] ENST00000429635; ENSP00000406020; ENSG00000139211; Homo sapiens. [Genome view]
GeneID	347902.
KEGG	hsa:347902.
UCSC	uc001rpk.1. human.
Organism-specific databases
CTD	347902.
GeneCards	GC12M045755.
H-InvDB	HIX0010573.
HGNC	HGNC:24073. AMIGO2.
PharmGKB	PA142672626.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q86SJ2.
HOVERGEN	Q86SJ2.
OMA	SCIAMNR
OrthoDB	EOG9HQH4J
Gene expression databases
ArrayExpress	Q86SJ2.
Bgee	Q86SJ2.
CleanEx	HS_AMIGO2.
Genevestigator	Q86SJ2.
GermOnline	ENSG00000139211. Homo sapiens.
Family and domain databases
InterPro	IPR013151. Ig. IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR001611. Leu-rich_rpt. IPR000372. Leu-rich_rpt_Cys-rich-dom_N. IPR003591. Leu-rich_rpt_typical-subtyp. IPR000483. LRR_C. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00047. ig. 1 hit. PF00560. LRR_1. 3 hits. [Graphical view]
SMART	SM00409. IG. 1 hit. SM00369. LRR_TYP. 1 hit. SM00082. LRRCT. 1 hit. SM00013. LRRNT. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	99311.

Entry information

Entry name

AMGO2_HUMAN

Accession

Primary (citable) accession number: Q86SJ2
Secondary accession number(s): Q4VBP6, Q7Z4A0, Q96CN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 1, 2003
Last modified:	November 24, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.