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Q86SJ2 (AMGO2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amphoterin-induced protein 2
Alternative name(s):
AMIGO-2
Alivin-1
Differentially expressed in gastric adenocarcinomas
Short name=DEGA
Gene names
Name:AMIGO2
Synonyms:ALI1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length522 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas. Ref.3 UniProtKB Q7TNJ4

Subunit structure

Binds itself as well as AMIGO1 and AMIGO3 By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity. Nucleus By similarity. Note: Associated with nucleus as well as plasma membrane. Restricted to somata of cerebellar as well as hippocampal neurons By similarity.

Tissue specificity

Highest levels in breast, ovary, cervix, and uterus. Lower levels in lung, colon, and rectum. Differentially expressed in 56% of thyroid, 57% of pancreatic and 45% of stomach cancers. Ref.3

Sequence similarities

Belongs to the immunoglobulin superfamily. AMIGO family.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 6 LRR (leucine-rich) repeats.

Contains 1 LRRCT domain.

Contains 1 LRRNT domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CACNA1AO005552EBI-3866830,EBI-766279

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3939 Potential
Chain40 – 522483Amphoterin-induced protein 2
PRO_0000014509

Regions

Topological domain40 – 398359Extracellular Potential
Transmembrane399 – 41921Helical; Potential
Topological domain420 – 522103Cytoplasmic Potential
Domain40 – 6829LRRNT
Repeat69 – 9022LRR 1
Repeat94 – 11522LRR 2
Repeat118 – 13922LRR 3
Repeat142 – 16322LRR 4
Repeat166 – 18722LRR 5
Repeat193 – 21422LRR 6
Domain228 – 28457LRRCT
Domain289 – 37991Ig-like C2-type

Amino acid modifications

Glycosylation581N-linked (GlcNAc...) Potential
Glycosylation1041N-linked (GlcNAc...) Potential
Glycosylation2811N-linked (GlcNAc...) Potential
Glycosylation2881N-linked (GlcNAc...) Potential
Glycosylation3451N-linked (GlcNAc...) Potential
Glycosylation3731N-linked (GlcNAc...) Potential
Glycosylation3811N-linked (GlcNAc...) Potential
Glycosylation3841N-linked (GlcNAc...) Potential
Disulfide bond41 ↔ 47 By similarity
Disulfide bond45 ↔ 54 By similarity
Disulfide bond232 ↔ 260 By similarity
Disulfide bond234 ↔ 282 By similarity
Disulfide bond310 ↔ 363 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q86SJ2 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 20A86E3D82C05B9B

FASTA52257,934
        10         20         30         40         50         60 
MSLRVHTLPT LLGAVVRPGC RELLCLLMIT VTVGPGASGV CPTACICATD IVSCTNKNLS 

        70         80         90        100        110        120 
KVPGNLFRLI KRLDLSYNRI GLLDSEWIPV SFAKLNTLIL RHNNITSIST GSFSTTPNLK 

       130        140        150        160        170        180 
CLDLSSNKLK TVKNAVFQEL KVLEVLLLYN NHISYLDPSA FGGLSQLQKL YLSGNFLTQF 

       190        200        210        220        230        240 
PMDLYVGRFK LAELMFLDVS YNRIPSMPMH HINLVPGKQL RGIYLHGNPF VCDCSLYSLL 

       250        260        270        280        290        300 
VFWYRRHFSS VMDFKNDYTC RLWSDSRHSR QVLLLQDSFM NCSDSIINGS FRALGFIHEA 

       310        320        330        340        350        360 
QVGERLMVHC DSKTGNANTD FIWVGPDNRL LEPDKEMENF YVFHNGSLVI ESPRFEDAGV 

       370        380        390        400        410        420 
YSCIAMNKQR LLNETVDVTI NVSNFTVSRS HAHEAFNTAF TTLAACVASI VLVLLYLYLT 

       430        440        450        460        470        480 
PCPCKCKTKR QKNMLHQSNA HSSILSPGPA SDASADERKA GAGKRVVFLE PLKDTAAGQN 

       490        500        510        520 
GKVRLFPSEA VIAEGILKST RGKSDSDSVN SVFSDTPFVA ST 

« Hide

References

« Hide 'large scale' references
[1]"AMIGO, a transmembrane protein implicated in axon tract development, defines a novel protein family with leucine-rich repeats."
Kuja-Panula J., Kiiltomaeki M., Yamashiro T., Rouhiainen A., Rauvala H.
J. Cell Biol. 160:963-973(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Fibrosarcoma.
[2]"Alivin 1, a novel neuronal activity-dependent gene, inhibits apoptosis and promotes survival of cerebellar granule neurons."
Ono T., Sekino-Suzuki N., Kikkawa Y., Yonekawa H., Kawashima S.
J. Neurosci. 23:5887-5896(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"DEGA/AMIGO-2, a leucine-rich repeat family member, differentially expressed in human gastric adenocarcinoma: effects on ploidy, chromosomal stability, cell adhesion/migration and tumorigenicity."
Rabenau K.E., O'Toole J.M., Bassi R., Kotanides H., Witte L., Ludwig D.L., Pereira D.S.
Oncogene 23:5056-5067(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Stomach.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis and Uterus.
[6]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY237005 mRNA. Translation: AAO48946.1.
AB079074 mRNA. Translation: BAC81188.1.
AB080610 mRNA. Translation: BAC81189.1.
AY454159 mRNA. Translation: AAR83271.1.
AL833007 mRNA. Translation: CAH56293.1.
BC014103 mRNA. Translation: AAH14103.2.
BC047595 mRNA. Translation: AAH47595.1.
BC095477 mRNA. Translation: AAH95477.1.
RefSeqNP_001137140.1. NM_001143668.1.
NP_862830.1. NM_181847.4.
XP_005268894.1. XM_005268837.1.
UniGeneHs.121520.

3D structure databases

ProteinModelPortalQ86SJ2.
SMRQ86SJ2. Positions 41-387.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid131497. 5 interactions.
IntActQ86SJ2. 1 interaction.
STRING9606.ENSP00000266581.

Polymorphism databases

DMDM68052338.

Proteomic databases

PaxDbQ86SJ2.
PeptideAtlasQ86SJ2.
PRIDEQ86SJ2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000266581; ENSP00000266581; ENSG00000139211.
ENST00000321382; ENSP00000320848; ENSG00000139211.
ENST00000429635; ENSP00000406020; ENSG00000139211.
ENST00000550413; ENSP00000449034; ENSG00000139211.
GeneID347902.
KEGGhsa:347902.
UCSCuc001rpk.3. human.

Organism-specific databases

CTD347902.
GeneCardsGC12M047469.
HGNCHGNC:24073. AMIGO2.
HPAHPA054004.
neXtProtNX_Q86SJ2.
PharmGKBPA142672626.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG146733.
HOGENOMHOG000231327.
HOVERGENHBG080231.
InParanoidQ86SJ2.
OMASCIAMNR.
OrthoDBEOG71CFKP.
PhylomeDBQ86SJ2.
TreeFamTF326838.

Gene expression databases

ArrayExpressQ86SJ2.
BgeeQ86SJ2.
CleanExHS_AMIGO2.
GenevestigatorQ86SJ2.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR000483. Cys-rich_flank_reg_C.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRR-contain_N.
[Graphical view]
PfamPF00560. LRR_1. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
SM00369. LRR_TYP. 1 hit.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
PS51450. LRR. 7 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi347902.
NextBio99311.
PROQ86SJ2.

Entry information

Entry nameAMGO2_HUMAN
AccessionPrimary (citable) accession number: Q86SJ2
Secondary accession number(s): Q4VBP6, Q7Z4A0, Q96CN8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM