Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q86SE8

- NPM2_HUMAN

UniProt

Q86SE8 - NPM2_HUMAN

Protein

Nucleoplasmin-2

Gene

NPM2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Core histones chaperone involved in chromatin reprogramming, specially during fertilization and early embryonic development. Probably involved in sperm DNA decondensation during fertilization.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei57 – 571Interaction between pentamers
    Sitei84 – 841Interaction between pentamers

    GO - Molecular functioni

    1. histone binding Source: UniProtKB
    2. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. chromatin remodeling Source: UniProtKB
    2. embryo development Source: UniProtKB
    3. oocyte differentiation Source: UniProtKB
    4. positive regulation of catalytic activity Source: Ensembl
    5. positive regulation of DNA replication Source: Ensembl
    6. positive regulation of meiosis Source: UniProtKB
    7. protein homooligomerization Source: Ensembl
    8. regulation of exit from mitosis Source: UniProtKB
    9. single fertilization Source: UniProtKB

    Keywords - Molecular functioni

    Chaperone, Chromatin regulator, Developmental protein

    Keywords - Biological processi

    Fertilization

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoplasmin-2
    Gene namesi
    Name:NPM2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:7930. NPM2.

    Subcellular locationi

    Nucleus By similarity
    Note: Found in the oocyte nucleus before nuclear membrane breakdown, after which it is redistributed to the cytoplasm.By similarity

    GO - Cellular componenti

    1. cytoplasmic chromatin Source: UniProtKB
    2. nuclear chromatin Source: UniProtKB
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31732.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 214214Nucleoplasmin-2PRO_0000219487Add
    BLAST

    Proteomic databases

    MaxQBiQ86SE8.
    PRIDEiQ86SE8.

    PTM databases

    PhosphoSiteiQ86SE8.

    Expressioni

    Gene expression databases

    ArrayExpressiQ86SE8.
    BgeeiQ86SE8.
    CleanExiHS_NPM2.
    GenevestigatoriQ86SE8.

    Organism-specific databases

    HPAiHPA041070.

    Interactioni

    Subunit structurei

    Homopentamer, when bound to H2A-H2B dimers only. Homodecamer of two stacked pentamers, when bound to H2A-H2B dimers and H3-H4 tetramers simultaneously.1 Publication

    Protein-protein interaction databases

    BioGridi115641. 1 interaction.
    IntActiQ86SE8. 1 interaction.
    STRINGi9606.ENSP00000289820.

    Structurei

    Secondary structure

    1
    214
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi16 – 238
    Beta strandi29 – 324
    Beta strandi43 – 519
    Beta strandi60 – 656
    Beta strandi78 – 847
    Turni85 – 873
    Beta strandi90 – 989
    Beta strandi100 – 10910
    Beta strandi113 – 1208

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3T30X-ray1.90A/B/C/D/E/F/G/H/I/J14-122[»]
    ProteinModelPortaliQ86SE8.
    SMRiQ86SE8. Positions 15-121.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni129 – 15224Acidic tract A2Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi165 – 18016Bipartite nuclear localization signalBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi129 – 14416Poly-GluAdd
    BLAST
    Compositional biasi177 – 1804Poly-Lys
    Compositional biasi182 – 1876Poly-Glu

    Domaini

    The acidic tract A2 mediates histone binding.1 Publication

    Sequence similaritiesi

    Belongs to the nucleoplasmin family.Curated

    Phylogenomic databases

    eggNOGiNOG78586.
    HOGENOMiHOG000056457.
    HOVERGENiHBG045601.
    InParanoidiQ86SE8.
    KOiK11277.
    OMAiSTICLGE.
    PhylomeDBiQ86SE8.
    TreeFamiTF327704.

    Family and domain databases

    Gene3Di2.60.120.340. 1 hit.
    InterProiIPR004301. Nucleoplasmin.
    IPR024057. Nucleoplasmin_core_dom.
    [Graphical view]
    PANTHERiPTHR22747. PTHR22747. 1 hit.
    SUPFAMiSSF69203. SSF69203. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q86SE8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNLSSASSTE EKAVTTVLWG CELSQERRTW TFRPQLEGKQ SCRLLLHTIC    50
    LGEKAKEEMH RVEILPPANQ EDKKMQPVTI ASLQASVLPM VSMVGVQLSP 100
    PVTFQLRAGS GPVFLSGQER YEASDLTWEE EEEEEGEEEE EEEEDDEDED 150
    ADISLEEQSP VKQVKRLVPQ KQASVAKKKK LEKEEEEIRA SVRDKSPVKK 200
    AKATARAKKP GFKK 214
    Length:214
    Mass (Da):24,152
    Last modified:June 1, 2003 - v1
    Checksum:iEAE3BEF1DC8E1CC1
    GO
    Isoform 2 (identifier: Q86SE8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         123-214: ASDLTWEEEE...ARAKKPGFKK → KKAGKRRRGNKSQR

    Note: No experimental confirmation available.

    Show »
    Length:136
    Mass (Da):15,238
    Checksum:iCEEABE3645C15B3B
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei123 – 21492ASDLT…PGFKK → KKAGKRRRGNKSQR in isoform 2. 1 PublicationVSP_054261Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY262113 mRNA. Translation: AAP33134.1.
    AY262114 mRNA. Translation: AAP33135.1.
    AK094267 mRNA. Translation: BAG52852.1.
    AC091171 Genomic DNA. No translation available.
    CH471080 Genomic DNA. Translation: EAW63731.1.
    CH471080 Genomic DNA. Translation: EAW63732.1.
    BC068078 mRNA. Translation: AAH68078.1.
    CCDSiCCDS6018.1. [Q86SE8-1]
    RefSeqiNP_001273609.1. NM_001286680.1. [Q86SE8-1]
    NP_001273610.1. NM_001286681.1. [Q86SE8-2]
    NP_877724.1. NM_182795.1. [Q86SE8-1]
    UniGeneiHs.131055.

    Genome annotation databases

    EnsembliENST00000289820; ENSP00000289820; ENSG00000158806. [Q86SE8-1]
    ENST00000381530; ENSP00000370941; ENSG00000158806. [Q86SE8-2]
    ENST00000397940; ENSP00000381032; ENSG00000158806. [Q86SE8-1]
    ENST00000518119; ENSP00000427741; ENSG00000158806. [Q86SE8-1]
    ENST00000521157; ENSP00000429413; ENSG00000158806. [Q86SE8-1]
    GeneIDi10361.
    KEGGihsa:10361.
    UCSCiuc003xac.3. human. [Q86SE8-1]

    Polymorphism databases

    DMDMi37537936.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY262113 mRNA. Translation: AAP33134.1 .
    AY262114 mRNA. Translation: AAP33135.1 .
    AK094267 mRNA. Translation: BAG52852.1 .
    AC091171 Genomic DNA. No translation available.
    CH471080 Genomic DNA. Translation: EAW63731.1 .
    CH471080 Genomic DNA. Translation: EAW63732.1 .
    BC068078 mRNA. Translation: AAH68078.1 .
    CCDSi CCDS6018.1. [Q86SE8-1 ]
    RefSeqi NP_001273609.1. NM_001286680.1. [Q86SE8-1 ]
    NP_001273610.1. NM_001286681.1. [Q86SE8-2 ]
    NP_877724.1. NM_182795.1. [Q86SE8-1 ]
    UniGenei Hs.131055.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3T30 X-ray 1.90 A/B/C/D/E/F/G/H/I/J 14-122 [» ]
    ProteinModelPortali Q86SE8.
    SMRi Q86SE8. Positions 15-121.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115641. 1 interaction.
    IntActi Q86SE8. 1 interaction.
    STRINGi 9606.ENSP00000289820.

    PTM databases

    PhosphoSitei Q86SE8.

    Polymorphism databases

    DMDMi 37537936.

    Proteomic databases

    MaxQBi Q86SE8.
    PRIDEi Q86SE8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000289820 ; ENSP00000289820 ; ENSG00000158806 . [Q86SE8-1 ]
    ENST00000381530 ; ENSP00000370941 ; ENSG00000158806 . [Q86SE8-2 ]
    ENST00000397940 ; ENSP00000381032 ; ENSG00000158806 . [Q86SE8-1 ]
    ENST00000518119 ; ENSP00000427741 ; ENSG00000158806 . [Q86SE8-1 ]
    ENST00000521157 ; ENSP00000429413 ; ENSG00000158806 . [Q86SE8-1 ]
    GeneIDi 10361.
    KEGGi hsa:10361.
    UCSCi uc003xac.3. human. [Q86SE8-1 ]

    Organism-specific databases

    CTDi 10361.
    GeneCardsi GC08P021881.
    HGNCi HGNC:7930. NPM2.
    HPAi HPA041070.
    MIMi 608073. gene.
    neXtProti NX_Q86SE8.
    PharmGKBi PA31732.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG78586.
    HOGENOMi HOG000056457.
    HOVERGENi HBG045601.
    InParanoidi Q86SE8.
    KOi K11277.
    OMAi STICLGE.
    PhylomeDBi Q86SE8.
    TreeFami TF327704.

    Miscellaneous databases

    ChiTaRSi NPM2. human.
    GenomeRNAii 10361.
    NextBioi 39277.
    PROi Q86SE8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q86SE8.
    Bgeei Q86SE8.
    CleanExi HS_NPM2.
    Genevestigatori Q86SE8.

    Family and domain databases

    Gene3Di 2.60.120.340. 1 hit.
    InterProi IPR004301. Nucleoplasmin.
    IPR024057. Nucleoplasmin_core_dom.
    [Graphical view ]
    PANTHERi PTHR22747. PTHR22747. 1 hit.
    SUPFAMi SSF69203. SSF69203. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Roles of NPM2 in chromatin and nucleolar organization in oocytes and embryos."
      Burns K.H., Viveiros M.M., Ren Y., Wang P., DeMayo F.J., Frail D.E., Eppig J.J., Matzuk M.M.
      Science 300:633-636(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Cerebellum.
    3. "DNA sequence and analysis of human chromosome 8."
      Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
      , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
      Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Skin.
    6. "New insights into the nucleophosmin/nucleoplasmin family of nuclear chaperones."
      Frehlick L.J., Eirin-Lopez J.M., Ausio J.
      Bioessays 29:49-59(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON FUNCTION.
    7. "Crystal structure and function of human nucleoplasmin (npm2): a histone chaperone in oocytes and embryos."
      Platonova O., Akey I.V., Head J.F., Akey C.W.
      Biochemistry 50:8078-8089(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 14-122, FUNCTION, DOMAIN, SUBUNIT.

    Entry informationi

    Entry nameiNPM2_HUMAN
    AccessioniPrimary (citable) accession number: Q86SE8
    Secondary accession number(s): B3KSU0, D3DSQ8, Q6NVH6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3