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Q86SE8 (NPM2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleoplasmin-2
Gene names
Name:NPM2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length214 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Core histones chaperone involved in chromatin reprogramming, specially during fertilization and early embryonic development. Probably involved in sperm DNA decondensation during fertilization. Ref.7

Subunit structure

Homopentamer, when bound to H2A-H2B dimers only. Homodecamer of two stacked pentamers, when bound to H2A-H2B dimers and H3-H4 tetramers simultaneously. Ref.7

Subcellular location

Nucleus By similarity. Note: Found in the oocyte nucleus before nuclear membrane breakdown, after which it is redistributed to the cytoplasm By similarity.

Domain

The acidic tract A2 mediates histone binding. Ref.7

Sequence similarities

Belongs to the nucleoplasmin family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q86SE8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q86SE8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     123-214: ASDLTWEEEE...ARAKKPGFKK → KKAGKRRRGNKSQR
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 214214Nucleoplasmin-2
PRO_0000219487

Regions

Region129 – 15224Acidic tract A2
Motif165 – 18016Bipartite nuclear localization signal By similarity
Compositional bias129 – 14416Poly-Glu
Compositional bias177 – 1804Poly-Lys
Compositional bias182 – 1876Poly-Glu

Sites

Site571Interaction between pentamers
Site841Interaction between pentamers

Natural variations

Alternative sequence123 – 21492ASDLT…PGFKK → KKAGKRRRGNKSQR in isoform 2.
VSP_054261

Secondary structure

.................. 214
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: EAE3BEF1DC8E1CC1

FASTA21424,152
        10         20         30         40         50         60 
MNLSSASSTE EKAVTTVLWG CELSQERRTW TFRPQLEGKQ SCRLLLHTIC LGEKAKEEMH 

        70         80         90        100        110        120 
RVEILPPANQ EDKKMQPVTI ASLQASVLPM VSMVGVQLSP PVTFQLRAGS GPVFLSGQER 

       130        140        150        160        170        180 
YEASDLTWEE EEEEEGEEEE EEEEDDEDED ADISLEEQSP VKQVKRLVPQ KQASVAKKKK 

       190        200        210 
LEKEEEEIRA SVRDKSPVKK AKATARAKKP GFKK 

« Hide

Isoform 2 [UniParc].

Checksum: CEEABE3645C15B3B
Show »

FASTA13615,238

References

« Hide 'large scale' references
[1]"Roles of NPM2 in chromatin and nucleolar organization in oocytes and embryos."
Burns K.H., Viveiros M.M., Ren Y., Wang P., DeMayo F.J., Frail D.E., Eppig J.J., Matzuk M.M.
Science 300:633-636(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Cerebellum.
[3]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Skin.
[6]"New insights into the nucleophosmin/nucleoplasmin family of nuclear chaperones."
Frehlick L.J., Eirin-Lopez J.M., Ausio J.
Bioessays 29:49-59(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW ON FUNCTION.
[7]"Crystal structure and function of human nucleoplasmin (npm2): a histone chaperone in oocytes and embryos."
Platonova O., Akey I.V., Head J.F., Akey C.W.
Biochemistry 50:8078-8089(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 14-122, FUNCTION, DOMAIN, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY262113 mRNA. Translation: AAP33134.1.
AY262114 mRNA. Translation: AAP33135.1.
AK094267 mRNA. Translation: BAG52852.1.
AC091171 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63731.1.
CH471080 Genomic DNA. Translation: EAW63732.1.
BC068078 mRNA. Translation: AAH68078.1.
CCDSCCDS6018.1.
RefSeqNP_001273609.1. NM_001286680.1. [Q86SE8-1]
NP_001273610.1. NM_001286681.1. [Q86SE8-2]
NP_877724.1. NM_182795.1. [Q86SE8-1]
UniGeneHs.131055.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3T30X-ray1.90A/B/C/D/E/F/G/H/I/J14-122[»]
ProteinModelPortalQ86SE8.
SMRQ86SE8. Positions 15-121.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115641. 1 interaction.
IntActQ86SE8. 1 interaction.
STRING9606.ENSP00000289820.

PTM databases

PhosphoSiteQ86SE8.

Polymorphism databases

DMDM37537936.

Proteomic databases

MaxQBQ86SE8.
PRIDEQ86SE8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000289820; ENSP00000289820; ENSG00000158806.
ENST00000381530; ENSP00000370941; ENSG00000158806.
ENST00000397940; ENSP00000381032; ENSG00000158806.
ENST00000518119; ENSP00000427741; ENSG00000158806.
ENST00000521157; ENSP00000429413; ENSG00000158806.
GeneID10361.
KEGGhsa:10361.
UCSCuc003xac.3. human. [Q86SE8-1]

Organism-specific databases

CTD10361.
GeneCardsGC08P021881.
HGNCHGNC:7930. NPM2.
HPAHPA041070.
MIM608073. gene.
neXtProtNX_Q86SE8.
PharmGKBPA31732.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG78586.
HOGENOMHOG000056457.
HOVERGENHBG045601.
InParanoidQ86SE8.
KOK11277.
OMASTICLGE.
PhylomeDBQ86SE8.
TreeFamTF327704.

Gene expression databases

ArrayExpressQ86SE8.
BgeeQ86SE8.
CleanExHS_NPM2.
GenevestigatorQ86SE8.

Family and domain databases

Gene3D2.60.120.340. 1 hit.
InterProIPR004301. Nucleoplasmin.
IPR024057. Nucleoplasmin_core_dom.
[Graphical view]
PANTHERPTHR22747. PTHR22747. 1 hit.
SUPFAMSSF69203. SSF69203. 1 hit.
ProtoNetSearch...

Other

ChiTaRSNPM2. human.
GenomeRNAi10361.
NextBio39277.
PROQ86SE8.
SOURCESearch...

Entry information

Entry nameNPM2_HUMAN
AccessionPrimary (citable) accession number: Q86SE8
Secondary accession number(s): B3KSU0, D3DSQ8, Q6NVH6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM