Q86S66 (NACA_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nascent polypeptide-associated complex subunit alpha
Short name=NAC-alpha

Alternative name(s):
Alpha-NAC

Gene names

ORF Names:

Y65B4BR.5

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May promote appropriate targeting of ribosome-nascent polypeptide complexes By similarity.
Sequence similarities	Belongs to the NAC-alpha family. Contains 1 NAC-A/B (NAC-alpha/beta) domain. Contains 1 UBA domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	body morphogenesis Inferred from mutant phenotype. Source: WormBase determination of adult lifespan Inferred from mutant phenotype. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase inductive cell migration Inferred from mutant phenotype. Source: WormBase lipid storage Inferred from mutant phenotype. Source: WormBase meiotic chromosome segregation Inferred from mutant phenotype. Source: WormBase molting cycle, collagen and cuticulin-based cuticle Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
F49E8.7	Q20632	5	EBI-326549,EBI-326535
icd-1	Q18885	5	EBI-326549,EBI-326530

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q86S66-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Gene prediction based on EST data. No experimental confirmation.

Isoform b (identifier: Q86S66-2)

The sequence of this isoform differs from the canonical sequence as follows:
     151-152: Missing.

Note: Gene prediction based on EST data. No experimental confirmation.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	Nascent polypeptide-associated complex subunit alpha		PRO_0000135585
Regions
	Domain	56 – 121	66	NAC-A/B
	Domain	158 – 195	38	UBA
Natural variations
	Alternative sequence	151 – 152	2	Missing in isoform b.		VSP_014887

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 8512718E4D7CA97C	FASTA	197	22,078
	10 20 30 40 50 60 MTGSTETRQK EVKEPQVDVS DDSDNEAVEQ ELTEEQRRVA EAAGLGDHID KQAKQSRSEK 70 80 90 100 110 120 KARKLFSKLG LKQVTGVSRV CIRKSKNILF VINKPDVFKS PGSDTYIIFG EAKIEDLTQH 130 140 150 160 170 180 AQMSAIENLK PTREAPQLKT VEEDENEDVE FKEDSTGIEE KDIELVISQA NTTRNKAIRA 190 LKEADNDIVN AIMSLTM
	Isoform b [UniParc]. Checksum: 0158E4BCCC1775B0	FASTA	195	21,802
	10 20 30 40 50 60 MTGSTETRQK EVKEPQVDVS DDSDNEAVEQ ELTEEQRRVA EAAGLGDHID KQAKQSRSEK 70 80 90 100 110 120 KARKLFSKLG LKQVTGVSRV CIRKSKNILF VINKPDVFKS PGSDTYIIFG EAKIEDLTQH 130 140 150 160 170 180 AQMSAIENLK PTREAPQLKT VEEDENEDVE EDSTGIEEKD IELVISQANT TRNKAIRALK 190 EADNDIVNAI MSLTM

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO081483 Genomic DNA. Translation: CCD71933.1. FO081483 Genomic DNA. Translation: CCD71934.1.
RefSeq	NP_490749.1. NM_058348.4. NP_871846.1. NM_182046.5.
UniGene	Cel.7722.
3D structure databases
ProteinModelPortal	Q86S66.
SMR	Q86S66. Positions 66-195.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-25064N.
IntAct	Q86S66. 9 interactions.
MINT	MINT-1063140.
STRING	Q86S66.
Proteomic databases
PRIDE	Q86S66.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	Y65B4BR.5b; Y65B4BR.5b; Y65B4BR.5.
GeneID	171646.
KEGG	cel:Y65B4BR.5.
UCSC	Y65B4BR.5a.1. c. elegans.
Organism-specific databases
CTD	171646.
WormBase	Y65B4BR.5a; CE22740; WBGene00022042. Y65B4BR.5b; CE33239; WBGene00022042.
Phylogenomic databases
eggNOG	meNOG12756.
GeneTree	EMGT00050000012327.
HOGENOM	HBG628290.
InParanoid	Q86S66.
OMA	ANTTRNK.
PhylomeDB	Q86S66.
Gene expression databases
ArrayExpress	Q86S66.
Family and domain databases
InterPro	IPR002715. Nas_poly-pep-assoc_cplx. IPR016641. Nas_poly-pep-assoc_cplx_asu. IPR000449. UBA/transl_elong_EF1B_N. [Graphical view]
KO	K03626.
Pfam	PF01849. NAC. 1 hit. PF00627. UBA. 1 hit. [Graphical view]
PIRSF	PIRSF015901. NAC_alpha. 1 hit.
PROSITE	PS51151. NAC_AB. 1 hit. PS50030. UBA. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	872129.

Entry information

Entry name

NACA_CAEEL

Accession

Primary (citable) accession number: Q86S66
Secondary accession number(s): Q9N2Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	June 1, 2003
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project