Q86S40 (ADPGK_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ADP-dependent glucokinase
Short name=ADP-GK
Short name=ADPGK
EC=2.7.1.147

Gene names

ORF Names:

C50D2.7

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency By similarity.
Catalytic activity	ADP + D-glucose = AMP + D-glucose 6-phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Carbohydrate degradation; glycolysis.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the ADP-dependent glucokinase family. Contains 1 ADPK (ADP-dependent kinase) domain.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Secreted
Domain	Signal
Ligand	Magnesium Metal-binding
Molecular function	Kinase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ADP-specific glucokinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 502	470	Probable ADP-dependent glucokinase		PRO_0000346790
Regions
	Domain	44 – 497	454	ADPK
Sites
	Active site	481	1	Proton acceptor By similarity
	Metal binding	290	1	Magnesium By similarity
	Metal binding	320	1	Magnesium By similarity
	Metal binding	481	1	Magnesium By similarity
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Ref.2
	Glycosylation	190	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q86S40 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1D9B57E5382FFF73	FASTA	502	56,762
	10 20 30 40 50 60 MFSETFVPSI FSYKHRLLHL SVLFFIVPYW YSYYNDQHRL SSYSVETAMF LSWERAIVKP 70 80 90 100 110 120 GAMFKKAVIG FNCNVDLIVS GVRVVDALNT TCSEGKDQET LETLADLHQT FAHFFQRGAA 130 140 150 160 170 180 AERYMSSEDQ FNLLVAESEA STRSHHHIGG NAALMADRIA ANFPSTEVYL VGPIGPRSQA 190 200 210 220 230 240 LLHPSVKRTN STRILKDELH VILEYKQGEI LGDWVAPSSS RFITSHDHFS GSMVVMEMFF 250 260 270 280 290 300 KAIAQFRPDL VVITGVHLLE FQSKEMRQEK MRLIKRNLLQ IPPKVPIHLE LGSLADEIFS 310 320 330 340 350 360 TDVINKILPY VDSLGINEQE LTFLSHIANG PHMEEYPVQA GTVHVHKVVE MLHWLLKTYG 370 380 390 400 410 420 RDPTGQIASK TGYRLSRIHF HCLTYHIMVS SGTDWSNLAA GLAAGARIAG RLSCNIGANT 430 440 450 460 470 480 MDSELLEIRT PANFVLDKKI EKNYQFEAHN PIASWMREDV LFVFTPVLVC RLPSKTVGID 490 500 DAISATGLLY SQFYRLNRPT HW

References

[1]

"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

[2]

"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-89, MASS SPECTROMETRY. Strain: Bristol N2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080633 Genomic DNA. Translation: CCD65339.1.
RefSeq	NP_493657.2. NM_061256.3.
UniGene	Cel.17770.
3D structure databases
ProteinModelPortal	Q86S40.
ModBase	Search...
Protein-protein interaction databases
STRING	Q86S40.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C50D2.7.1; C50D2.7.1; C50D2.7. C50D2.7.2; C50D2.7.2; C50D2.7.
GeneID	173393.
KEGG	cel:C50D2.7.
UCSC	C50D2.7.1. c. elegans.
Organism-specific databases
CTD	173393.
WormBase	C50D2.7; CE33040; WBGene00016810.
Phylogenomic databases
eggNOG	meNOG04350.
GeneTree	EMGT00050000009808.
HOGENOM	HBG444580.
InParanoid	Q86S40.
OMA	NAALIGQ.
PhylomeDB	Q86S40.
Family and domain databases
InterPro	IPR007666. ADP_PFK/GK. [Graphical view]
KO	K08074.
PANTHER	PTHR21208. ADP_PFK/GK. 1 hit.
Pfam	PF04587. ADP_PFK_GK. 1 hit. [Graphical view]
PROSITE	PS51255. ADPK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	879463.

Entry information

Entry name

ADPGK_CAEEL

Accession

Primary (citable) accession number: Q86S40

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 1, 2003
Last modified:	December 14, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project