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Q86RQ1 (CLE2_HETGL) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CLAVATA3/ESR (CLE)-related protein 2

Short name=CLE-like peptide 2
Alternative name(s):
Gland-specific protein G4G12
Short name=Hg-G4G12
Gene names
Name:CLE2
Synonyms:G4G12
OrganismHeterodera glycines (Soybean cyst nematode worm)
Taxonomic identifier51029 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaTylenchidaTylenchinaTylenchoideaHeteroderidaeHeteroderinaeHeterodera

Protein attributes

Sequence length138 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mimics host plant CLE extracellular signal peptides that regulate cell fate. May play a role in the differentiation or division of feeding cells (syncytia) induced in plant roots during infection. Ref.2 Ref.3

Subcellular location

Secreted. Host cytoplasm. Host extracellular space. Secretedextracellular spaceapoplast. Note: Present in secretory granules within the dorsal esophageal gland secretory cell and in the dorsal gland ampulla (collecting reservoir) at the base of the nematode stylet. Secreted into host root cells via the nematode stylet to transform the recipient cells into enlarged multinucleate feeding cells called giant-cells or syncytia. Secreted to the host apoplasm from its cytoplasm via a plant secretory pathway. Ref.2 Ref.3

Tissue specificity

Highly expressed exclusively within the dorsal esophageal gland cell during syncytium formation in host plants (at protein level). Ref.1 Ref.2 Ref.3

Developmental stage

Strongly up-regulated during root colonization, from the onset of syncytium formation by parasitic second-stage juveniles (pJ2) through the J3?J4 molts of sedentary life stages that become adult females. Ref.2

Sequence similarities

Belongs to the CLV3/ESR signal peptide family.

Ontologies

Keywords
   Biological processDifferentiation
   Cellular componentApoplast
Host cytoplasm
Secreted
   DomainSignal
   PTMGlycoprotein
Gene Ontology (GO)
   Biological_processcell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular space of host

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 138116CLAVATA3/ESR (CLE)-related protein 2
PRO_0000401216

Regions

Region23 – 9068Required for secretion from the host cytoplasm to the host apoplasm
Motif127 – 13812CLE
Compositional bias92 – 965Poly-Pro

Amino acid modifications

Glycosylation371N-linked (GlcNAc...) Potential
Glycosylation871N-linked (GlcNAc...) Potential
Glycosylation1231N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q86RQ1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 7AA362FC5D9C38C8

FASTA13814,456
        10         20         30         40         50         60 
MPNIFKILLI VLLAVVSFRL SASTGDKKTA NDGSGNNSSA GIGTKIKRIV TAGLLFTSLA 

        70         80         90        100        110        120 
TGGAEAIGRS NAQGGNAAGL VPSHVTNRSM APPPPPVQFE MGANRLEKMR AHLRELAEKM 

       130 
PVNESKRLSP SGPDPHHH 

« Hide

References

[1]"The parasitome of the phytonematode Heterodera glycines."
Gao B., Allen R., Maier T., Davis E.L., Baum T.J., Hussey R.S.
Mol. Plant Microbe Interact. 16:720-726(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Esophagus and Gland.
[2]"Dual roles for the variable domain in protein trafficking and host-specific recognition of Heterodera glycines CLE effector proteins."
Wang J., Lee C., Replogle A., Joshi S., Korkin D., Hussey R., Baum T.J., Davis E.L., Wang X., Mitchum M.G.
New Phytol. 187:1003-1017(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
[3]"A parasitism gene from a plant-parasitic nematode with function similar to CLAVATA3/ESR (CLE) of Arabidopsis thaliana."
Wang X., Mitchum M.G., Gao B., Li C., Diab H., Baum T.J., Hussey R.S., Davis E.L.
Mol. Plant Pathol. 6:187-191(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]Erratum
Davis E.L.
Mol. Plant Pathol. 10:151-151(2009)
[5]"Diverse and conserved roles of CLE peptides."
Mitchum M.G., Wang X., Davis E.L.
Curr. Opin. Plant Biol. 11:75-81(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.

Web resources

Protein Spotlight

the root of the problem - Issue 151 of July 2013

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF473827 mRNA. Translation: AAO33474.1.
FJ503005 Genomic DNA. Translation: ACT32610.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameCLE2_HETGL
AccessionPrimary (citable) accession number: Q86RQ1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: June 1, 2003
Last modified: October 16, 2013
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries