Reviewed, UniProtKB/Swiss-Prot Q86M34 (HEXB_ENTHI)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase subunit beta
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase subunit beta
    Beta-GlcNAcase subunit beta
    Beta-N-acetylhexosaminidase subunit beta

Gene names

Name:

HEXB

Organism

Entamoeba histolytica

Taxonomic identifier

5759 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Archamoebae › Entamoebidae › Entamoeba

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hexoaminidase complex may contribute to amoebic pathogenicity and may be involved in the destruction of extracellular matrix components. Ref.1
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Subunit structure	Heterodimer of an alpha and a beta subunit.
Subcellular location	Cytoplasmic granule. Ref.1
Post-translational modification	Glycosylated. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 13	13	Ref.1
	Chain	14 – 565	552	Beta-hexosaminidase subunit beta		PRO_0000012012
Amino acid modifications
	Glycosylation	71	1	N-linked (GlcNAc...) Probable

Sequences

	Sequence		Length	Mass (Da)
	Q86M34-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: DABBBA0B932BBA4F	FASTA	565	64,372
	10 20 30 40 50 60 MIVLLLLISY CFAGNGVNVK NQLLLMPYPT TVNAQFGSND CVEATSNIKM VLSNNCQNDP 70 80 90 100 110 120 NCLSFMTFNF NHTITYPLQR QRNLEDFRVS IFAPIDIEEM KGNVVYSANT VNIELTGNNI 130 140 150 160 170 180 EEIYPPLKIG IDESYSLDVT KEGIKISATT VYGARLGLET LIQMLRPYQG KYIIKHIPIM 190 200 210 220 230 240 IEDKPRLQWR GLMIDVARNS FSRSAFVKII NAMAAIKANV LHIHLSDAQT FMFESKEYPE 250 260 270 280 290 300 LSKKGAFFQN KVLTQSFIKQ LVQYGAKRGV IVYPEIDTPA HTASWNAGYP GVVADIWDYI 310 320 330 340 350 360 VSSSMRYGEN VLALNPANEK TFSIIDALMK EMGEVFGNDY VHFGGDEVWT GAWSKAKEYP 370 380 390 400 410 420 AILEWMNKKG INTLKELEAY FNKYAQEQII KNGKTPVCWE EVYQKGSADK KTIIQVWNNV 430 440 450 460 470 480 NLLKEAATAG YKVILSAGYY LDMQMPLCSD YVADSCTNPN HMWVWTNRDM YRNDPIKELD 490 500 510 520 530 540 YATKQNVLGG EACSWDESVD EQNFFDRVFQ RFSAVAERFW SSEDITDPES HEVRANYVRC 550 560 LGLRRNFLKG TGPLYHSYCQ LPEDI

References

[1]

"The beta-N-acetylhexosaminidase of Entamoeba histolytica is composed of two homologous chains and has been localized to cytoplasmic granules." Riekenberg S., Flockenhaus B., Vahrmann A., Mueller M.C.M., Leippe M., Kiess M., Scholze H.H. Mol. Biochem. Parasitol. 138:217-225(2004) [PubMed: 15555733] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 14-33; 146-155 AND 307-316, FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH HEXA. Strain: HM-1:IMSS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ417748 Genomic DNA. Translation: CAD10500.3.
3D structure databases
HSSP	HSSP built from PDB template 1NOW based on UniProtKB P07686.
ModBase	Search...
Protein family/group databases
CAZy	GH20. Glycoside Hydrolase Family 20.
Enzyme and pathway databases
BRENDA	3.2.1.52. 323.
Family and domain databases
InterPro	IPR001540. Glyco_hydro_20. IPR015883. Glyco_hydro_20_cat-core. IPR013781. Glyco_hydro_sg_catalytic. IPR015882. HexNAc-like_b. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR22600. Glyco_hydro_20. 1 hit.
Pfam	PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. [Graphical view]
PRINTS	PR00738. GLHYDRLASE20.
ProtoNet	Search...

Entry information

Entry name

HEXB_ENTHI

Accession

Primary (citable) accession number: Q86M34

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)