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Q86M34 (HEXB_ENTHI) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase subunit beta

EC=3.2.1.52
Alternative name(s):
Beta-GlcNAcase subunit beta
Beta-N-acetylhexosaminidase subunit beta
N-acetyl-beta-glucosaminidase subunit beta
Gene names
Name:HEXB
OrganismEntamoeba histolytica
Taxonomic identifier5759 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba

Protein attributes

Sequence length565 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hexoaminidase complex may contribute to amoebic pathogenicity and may be involved in the destruction of extracellular matrix components. Ref.1

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

Subunit structure

Heterodimer of an alpha and a beta subunit.

Subcellular location

Cytoplasmic granule Ref.1.

Post-translational modification

Glycosylated. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-N-acetylhexosaminidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1313 Ref.1
Chain14 – 565552Beta-hexosaminidase subunit beta
PRO_0000012012

Amino acid modifications

Glycosylation711N-linked (GlcNAc...) Probable

Sequences

Sequence LengthMass (Da)Tools
Q86M34 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: DABBBA0B932BBA4F

FASTA56564,372
        10         20         30         40         50         60 
MIVLLLLISY CFAGNGVNVK NQLLLMPYPT TVNAQFGSND CVEATSNIKM VLSNNCQNDP 

        70         80         90        100        110        120 
NCLSFMTFNF NHTITYPLQR QRNLEDFRVS IFAPIDIEEM KGNVVYSANT VNIELTGNNI 

       130        140        150        160        170        180 
EEIYPPLKIG IDESYSLDVT KEGIKISATT VYGARLGLET LIQMLRPYQG KYIIKHIPIM 

       190        200        210        220        230        240 
IEDKPRLQWR GLMIDVARNS FSRSAFVKII NAMAAIKANV LHIHLSDAQT FMFESKEYPE 

       250        260        270        280        290        300 
LSKKGAFFQN KVLTQSFIKQ LVQYGAKRGV IVYPEIDTPA HTASWNAGYP GVVADIWDYI 

       310        320        330        340        350        360 
VSSSMRYGEN VLALNPANEK TFSIIDALMK EMGEVFGNDY VHFGGDEVWT GAWSKAKEYP 

       370        380        390        400        410        420 
AILEWMNKKG INTLKELEAY FNKYAQEQII KNGKTPVCWE EVYQKGSADK KTIIQVWNNV 

       430        440        450        460        470        480 
NLLKEAATAG YKVILSAGYY LDMQMPLCSD YVADSCTNPN HMWVWTNRDM YRNDPIKELD 

       490        500        510        520        530        540 
YATKQNVLGG EACSWDESVD EQNFFDRVFQ RFSAVAERFW SSEDITDPES HEVRANYVRC 

       550        560 
LGLRRNFLKG TGPLYHSYCQ LPEDI 

« Hide

References

[1]"The beta-N-acetylhexosaminidase of Entamoeba histolytica is composed of two homologous chains and has been localized to cytoplasmic granules."
Riekenberg S., Flockenhaus B., Vahrmann A., Mueller M.C.M., Leippe M., Kiess M., Scholze H.H.
Mol. Biochem. Parasitol. 138:217-225(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 14-33; 146-155 AND 307-316, FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH HEXA.
Strain: ATCC 30459 / HM-1:IMSS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ417748 Genomic DNA. Translation: CAD10500.3.

3D structure databases

ProteinModelPortalQ86M34.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH20. Glycoside Hydrolase Family 20.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG3525.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR025705. Beta_hexosaminidase_sua/sub.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00728. Glyco_hydro_20. 1 hit.
[Graphical view]
PIRSFPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSPR00738. GLHYDRLASE20.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHEXB_ENTHI
AccessionPrimary (citable) accession number: Q86M34
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: June 1, 2003
Last modified: October 16, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries