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Protein

Bifunctional purine biosynthesis protein purH

Gene

purH

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route).
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein purH (purH)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein purH (purH)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Transferase
Biological processPurine biosynthesis

Enzyme and pathway databases

ReactomeiR-DDI-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00133
UPA00074; UER00135

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC:2.1.2.3)
Alternative name(s):
AICAR transformylase
IMP cyclohydrolase (EC:3.5.4.10)
Alternative name(s):
ATIC
IMP synthase
Inosinicase
Gene namesi
Name:purH
ORF Names:DDB_G0277087
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0277087 purH

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003290221 – 542Bifunctional purine biosynthesis protein purHAdd BLAST542

Proteomic databases

PaxDbiQ86L14

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0230095

Structurei

3D structure databases

ProteinModelPortaliQ86L14
SMRiQ86L14
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 143MGS-likePROSITE-ProRule annotationAdd BLAST143

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.By similarity

Sequence similaritiesi

Belongs to the PurH family.Curated

Phylogenomic databases

eggNOGiKOG2555 Eukaryota
COG0138 LUCA
InParanoidiQ86L14
KOiK00602
OMAiDLLFAWK
PhylomeDBiQ86L14

Family and domain databases

Gene3Di3.40.140.20, 2 hits
3.40.50.1380, 1 hit
HAMAPiMF_00139 PurH, 1 hit
InterProiView protein in InterPro
IPR024051 AICAR_Tfase_dup_dom_sf
IPR016193 Cytidine_deaminase-like
IPR011607 MGS-like_dom
IPR036914 MGS-like_dom_sf
IPR002695 PurH-like
PANTHERiPTHR11692 PTHR11692, 1 hit
PfamiView protein in Pfam
PF01808 AICARFT_IMPCHas, 1 hit
PF02142 MGS, 1 hit
PIRSFiPIRSF000414 AICARFT_IMPCHas, 1 hit
SMARTiView protein in SMART
SM00798 AICARFT_IMPCHas, 1 hit
SM00851 MGS, 1 hit
SUPFAMiSSF52335 SSF52335, 1 hit
SSF53927 SSF53927, 1 hit
TIGRFAMsiTIGR00355 purH, 1 hit
PROSITEiView protein in PROSITE
PS51855 MGS, 1 hit

Sequencei

Sequence statusi: Complete.

Q86L14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQALLSVYNK SGIVEFSKIL SSKGFNLIST GGTAKSLVDN GLKVQQVSDV
60 70 80 90 100
TEYPEMLDGR VKTLHPKIHG GLLARPELAH HQADLNKYNI KPISIVVVNL
110 120 130 140 150
YPFVETVSKE STTLEEAIEN IDIGGHTLIR ASSKNFQNVL IIVDPSDYKW
160 170 180 190 200
IGERIQSSTD STNVLSSITL EERKKLALKA FQHGCSYDAA VSQYLSKVEL
210 220 230 240 250
TNATTIQGVK GTDSASVNVE FPQTFLPLYE KKNDLRYGEN PHQKAALYQC
260 270 280 290 300
PGTGGIANAQ LLHGPALSYN NILDGDAALK AVREFDRCAC VVIKHTNPCG
310 320 330 340 350
LSVGVNDSEQ AEVYKRAFNG DPKSAYGGIL GFNRTLTLET ATALKSVFYE
360 370 380 390 400
VIIAPDYTED ALALLSKKEK LRILRIPEAA NQIQFTQPDI RTITGGALLQ
410 420 430 440 450
SPNPIIRGDL AEATKNWKVV TENKPTEQQM KDLLFAWRVS KHVKSNAIVL
460 470 480 490 500
SKDETIVAIG AGQPNRSQSV DICMKVGGDK VKGSVLASDA FFPFADSIDL
510 520 530 540
AHQGNIACIV QPGGSIRDQE VIDAANKYGI PMVFTGNRNF LH
Length:542
Mass (Da):59,087
Last modified:June 1, 2003 - v1
Checksum:iB2EC8F2D199D04F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000019 Genomic DNA Translation: EAL69045.1
RefSeqiXP_642943.1, XM_637851.1

Genome annotation databases

EnsemblProtistsiEAL69045; EAL69045; DDB_G0277087
GeneIDi8620812
KEGGiddi:DDB_G0277087

Similar proteinsi

Entry informationi

Entry nameiPUR9_DICDI
AccessioniPrimary (citable) accession number: Q86L14
Secondary accession number(s): Q550I9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: March 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health