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Q86L14

- PUR9_DICDI

UniProt

Q86L14 - PUR9_DICDI

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Protein

Bifunctional purine biosynthesis protein purH

Gene

purH

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

Pathwayi

GO - Molecular functioni

  1. IMP cyclohydrolase activity Source: dictyBase
  2. phosphoribosylaminoimidazolecarboxamide formyltransferase activity Source: dictyBase

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: dictyBase
  2. purine nucleotide biosynthetic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transferase

Keywords - Biological processi

Purine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC:2.1.2.3)
Alternative name(s):
AICAR transformylase
IMP cyclohydrolase (EC:3.5.4.10)
Alternative name(s):
ATIC
IMP synthase
Inosinicase
Gene namesi
Name:purH
ORF Names:DDB_G0277087
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0277087. purH.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: dictyBase
  2. phagocytic vesicle Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 542542Bifunctional purine biosynthesis protein purHPRO_0000329022Add
BLAST

Proteomic databases

PRIDEiQ86L14.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0230095.

Structurei

3D structure databases

ProteinModelPortaliQ86L14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.By similarity

Sequence similaritiesi

Belongs to the PurH family.Curated

Phylogenomic databases

eggNOGiCOG0138.
InParanoidiQ86L14.
KOiK00602.
OMAiIRASSKN.
PhylomeDBiQ86L14.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q86L14-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQALLSVYNK SGIVEFSKIL SSKGFNLIST GGTAKSLVDN GLKVQQVSDV
60 70 80 90 100
TEYPEMLDGR VKTLHPKIHG GLLARPELAH HQADLNKYNI KPISIVVVNL
110 120 130 140 150
YPFVETVSKE STTLEEAIEN IDIGGHTLIR ASSKNFQNVL IIVDPSDYKW
160 170 180 190 200
IGERIQSSTD STNVLSSITL EERKKLALKA FQHGCSYDAA VSQYLSKVEL
210 220 230 240 250
TNATTIQGVK GTDSASVNVE FPQTFLPLYE KKNDLRYGEN PHQKAALYQC
260 270 280 290 300
PGTGGIANAQ LLHGPALSYN NILDGDAALK AVREFDRCAC VVIKHTNPCG
310 320 330 340 350
LSVGVNDSEQ AEVYKRAFNG DPKSAYGGIL GFNRTLTLET ATALKSVFYE
360 370 380 390 400
VIIAPDYTED ALALLSKKEK LRILRIPEAA NQIQFTQPDI RTITGGALLQ
410 420 430 440 450
SPNPIIRGDL AEATKNWKVV TENKPTEQQM KDLLFAWRVS KHVKSNAIVL
460 470 480 490 500
SKDETIVAIG AGQPNRSQSV DICMKVGGDK VKGSVLASDA FFPFADSIDL
510 520 530 540
AHQGNIACIV QPGGSIRDQE VIDAANKYGI PMVFTGNRNF LH
Length:542
Mass (Da):59,087
Last modified:June 1, 2003 - v1
Checksum:iB2EC8F2D199D04F1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000019 Genomic DNA. Translation: EAL69045.1.
RefSeqiXP_642943.1. XM_637851.1.

Genome annotation databases

EnsemblProtistsiDDB0230095; DDB0230095; DDB_G0277087.
GeneIDi8620812.
KEGGiddi:DDB_G0277087.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000019 Genomic DNA. Translation: EAL69045.1 .
RefSeqi XP_642943.1. XM_637851.1.

3D structure databases

ProteinModelPortali Q86L14.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0230095.

Proteomic databases

PRIDEi Q86L14.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0230095 ; DDB0230095 ; DDB_G0277087 .
GeneIDi 8620812.
KEGGi ddi:DDB_G0277087.

Organism-specific databases

dictyBasei DDB_G0277087. purH.

Phylogenomic databases

eggNOGi COG0138.
InParanoidi Q86L14.
KOi K00602.
OMAi IRASSKN.
PhylomeDBi Q86L14.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00133 .
UPA00074 ; UER00135 .

Miscellaneous databases

PROi Q86L14.

Family and domain databases

Gene3Di 3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPi MF_00139. PurH.
InterProi IPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view ]
PANTHERi PTHR11692. PTHR11692. 1 hit.
Pfami PF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view ]
PIRSFi PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTi SM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view ]
SUPFAMi SSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsi TIGR00355. purH. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
    Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
    Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: AX2.

Entry informationi

Entry nameiPUR9_DICDI
AccessioniPrimary (citable) accession number: Q86L14
Secondary accession number(s): Q550I9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3