Reviewed, UniProtKB/Swiss-Prot Q86KU6 (MTD1_DICDI)

Names and origin

Protein names

Recommended name:
Methylenetetrahydrofolate dehydrogenase [NAD+]
EC=1.5.1.15

Gene names

Name:	thfA
ORF Names:	DDB_G0277725

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes oxidation of cytoplasmic one-carbon units for purine biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + NAD+ = 5,10-methenyltetrahydrofolate + NADH.
Cofactor	NAD By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Ontologies

Keywords
Biological process	One-carbon metabolism Purine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro methylenetetrahydrofolate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Methylenetetrahydrofolate dehydrogenase [NAD+]		PRO_0000327391
Regions
	Nucleotide binding	187 – 188	2	NAD By similarity
	Nucleotide binding	210 – 211	2	NAD By similarity
	Nucleotide binding	270 – 272	3	NAD By similarity
Sites
	Active site	152	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q86KU6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 90892D0167BCD840	FASTA	313	35,069
	10 20 30 40 50 60 MEEYKEIMQN KKGKVDVTPI ANFFREEVKQ QITKNCWKPR VVAFLTSDDQ GAIDYSKWTK 70 80 90 100 110 120 LACQKDGIEF ILKRVERVDL EDLIIEANND SCVHGILVYY PVFGGMMDSY LQDVVSPTKD 130 140 150 160 170 180 IEGLSTQNRF NLYHNIRFMD GETATKKCVI PCTPLAMVKI IDNLGIYDKS LAMGEHLKGK 190 200 210 220 230 240 TVTIVNRSEI VGRPLAAMLA NDGAIVYSID INGIIIFQSG KRHGTIKMSE TNVTREEAIS 250 260 270 280 290 300 KSDILILGVP SPNYKVNSDL IQDGTIVINF AGCLNVDESI QEKSILVPTI GKVTIAMLER 310 NLLRLFNNQI SNK

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000022 Genomic DNA. Translation: EAL68536.1.
RefSeq	XP_642462.1.
3D structure databases
HSSP	HSSP built from PDB template 1EDZ based on UniProtKB Q02046.
SMR	Q86KU6. Positions 11-309.
ModBase	Search...
Protein-protein interaction databases
STRING	Q86KU6.
Genome annotation databases
GeneID	8621173.
GenomeReviews	Gene locus thfA in contig CM000151_GR.
KEGG	ddi:DDB_0230118.
Organism-specific databases
dictyBase	DDB_G0277725. thfA.
Phylogenomic databases
eggNOG	KOG0089.
HOGENOM	HBG328751.
OMA	GNRLYGK.
PhylomeDB	Q86KU6.
ProtClustDB	CLSZ2430793.
Enzyme and pathway databases
BRENDA	1.5.1.15. 424.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR000672. THF_DH/CycHdrlase. IPR020630. THF_DH/CycHdrlase_cat_dom. IPR020631. THF_DH/CycHdrlase_NAD-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view]
PRINTS	PR00085. THFDHDRGNASE.
SUPFAM	SSF51735. NAD(P)-bd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTD1_DICDI

Accession

Primary (citable) accession number: Q86KU6
Secondary accession number(s): Q54Z91

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	March 2, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)