Q86JJ5 (RPB11_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase II subunit rpb11
Short name=RNA polymerase II subunit B11

Alternative name(s):
DNA-directed RNA polymerase II subunit J

Gene names

Name:	polr2j
Synonyms:	rpb11
ORF Names:	DDB_G0277677

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft By similarity.
Subunit structure	Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: dictyBase
Cellular_component	DNA-directed RNA polymerase II, core complex Inferred from sequence or structural similarity. Source: dictyBase
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 120	120	DNA-directed RNA polymerase II subunit rpb11		PRO_0000328750

Sequences

	Sequence		Length	Mass (Da)
	Q86JJ5 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 140A62ECE9C24E53	FASTA	120	13,894
	10 20 30 40 50 60 MNAPDRFELF VLPDGAKKVT MVRDTKIPNA SMFTILKEDH TIGNLIRMQL IADQDIIFAG 70 80 90 100 110 120 YRMPHPLEHN VNIRIQTNNN TNPLECVQKS MECLSREFTS LENSFIEQVQ KKRNVADQYI

References

[1]

"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000021 Genomic DNA. Translation: EAL68596.2.
RefSeq	XP_642530.2. XM_637438.2.
3D structure databases
ProteinModelPortal	Q86JJ5.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0216285.
Proteomic databases
PRIDE	Q86JJ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0216285; DDB0216285; DDB_G0277677.
GeneID	8621156.
KEGG	ddi:DDB_G0277677.
Organism-specific databases
dictyBase	DDB_G0277677. rpb11.
Phylogenomic databases
eggNOG	COG1761.
KO	K03008.
OMA	MNAPDRF.
ProtClustDB	CLSZ2431578.
Family and domain databases
InterPro	IPR011261. DNA-dir_RNA_pol_dimersation. IPR009025. DNA-dir_RNA_pol_RBP11-like. [Graphical view]
Pfam	PF01193. RNA_pol_L. 1 hit. [Graphical view]
SUPFAM	SSF55257. RNAP_RBP11-like. 1 hit.
PROSITE	PS01154. RNA_POL_L_13KD. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPB11_DICDI

Accession

Primary (citable) accession number: Q86JJ5
Secondary accession number(s): Q54ZA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)