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Q86IV5

- CTNA_DICDI

UniProt

Q86IV5 - CTNA_DICDI

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Protein

Countin-1

Gene

ctnA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cell-counting factor that limits the maximum size of the multicellular structure. May down-regulate the expression of gp24, which mediates cell adhesion.3 Publications

GO - Molecular functioni

  1. signal transducer activity Source: dictyBase

GO - Biological processi

  1. actin filament polymerization Source: dictyBase
  2. cellular component movement Source: dictyBase
  3. glucose homeostasis Source: dictyBase
  4. multicellular organismal development Source: dictyBase
  5. negative regulation of aggregate size involved in sorocarp development Source: dictyBase
  6. negative regulation of cell adhesion Source: dictyBase
  7. negative regulation of cGMP biosynthetic process Source: dictyBase
  8. regulation of glucose-6-phosphatase activity Source: dictyBase
  9. regulation of glycolytic process Source: dictyBase
  10. signal transduction Source: GOC
Complete GO annotation...

Protein family/group databases

TCDBi1.C.35.4.1. the amoebapore (amoebapore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Countin-1
Gene namesi
Name:ctnA
ORF Names:DDB_G0274597
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0274597. ctnA.

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Cells form a fewer number of larger aggregates.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 258237Countin-1PRO_0000327908Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi25 ↔ 102PROSITE-ProRule annotation
Disulfide bondi28 ↔ 96PROSITE-ProRule annotation
Disulfide bondi56 ↔ 69PROSITE-ProRule annotation
Glycosylationi121 – 1211N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi215 – 2151N-linked (GlcNAc...)PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ86IV5.

Expressioni

Developmental stagei

Highly expressed in the vegetative cells and decreases during subsequent developmental stages (6-24 hours).2 Publications

Interactioni

Subunit structurei

Component of the counting factor (CF) complex, which includes cf60, cf50, cf45-1 and ctnA.4 Publications

Protein-protein interaction databases

STRINGi44689.DDB_0185089.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 10685Saposin B-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the countin family.Curated
Contains 1 saposin B-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG12793.
InParanoidiQ86IV5.
OMAiCNGECGS.
PhylomeDBiQ86IV5.

Family and domain databases

InterProiIPR008139. SaposinB.
[Graphical view]
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
PROSITEiPS50015. SAP_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q86IV5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKLFSLILA LFLVNSAVVS SLDSCSICVD FVGNSLNDLL NIILNSGVIG
60 70 80 90 100
TCGDLCSAVP GGQIVDTVCD LLCDYVGVDE FIKLISDVDP DPIYICEKIS
110 120 130 140 150
VCKTNDNAAA SLDLVQINPQ NGTVGGTFTL SIAYTVTNTI ATGQLAFNII
160 170 180 190 200
DPTGNAFGDA VLLVQQSPEQ YTQQFQFQAT PSEQESFPNG LYTVQALVCE
210 220 230 240 250
GSCGSPHPNT YTLANGTTTF TISGADSSSM SGAGSFSGSS QSTQTGAASG

SGSGFALF
Length:258
Mass (Da):26,659
Last modified:June 1, 2003 - v1
Checksum:i725F37870E78356A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451Missing in AAD53482. (PubMed:10444594)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF140780 Genomic DNA. Translation: AAD53482.1.
AAFI02000012 Genomic DNA. Translation: EAL70191.1.
RefSeqiXP_643887.1. XM_638795.1.

Genome annotation databases

EnsemblProtistsiDDB0185089; DDB0185089; DDB_G0274597.
GeneIDi8619313.
KEGGiddi:DDB_G0274597.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF140780 Genomic DNA. Translation: AAD53482.1 .
AAFI02000012 Genomic DNA. Translation: EAL70191.1 .
RefSeqi XP_643887.1. XM_638795.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0185089.

Protein family/group databases

TCDBi 1.C.35.4.1. the amoebapore (amoebapore) family.

Proteomic databases

PRIDEi Q86IV5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0185089 ; DDB0185089 ; DDB_G0274597 .
GeneIDi 8619313.
KEGGi ddi:DDB_G0274597.

Organism-specific databases

dictyBasei DDB_G0274597. ctnA.

Phylogenomic databases

eggNOGi NOG12793.
InParanoidi Q86IV5.
OMAi CNGECGS.
PhylomeDBi Q86IV5.

Family and domain databases

InterProi IPR008139. SaposinB.
[Graphical view ]
SMARTi SM00741. SapB. 1 hit.
[Graphical view ]
PROSITEi PS50015. SAP_B. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A cell-counting factor regulating structure size in Dictyostelium."
    Brock D.A., Gomer R.H.
    Genes Dev. 13:1960-1969(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-41, IDENTIFICATION IN THE CF COMPLEX, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, FUNCTION, DISRUPTION PHENOTYPE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "A precise group size in Dictyostelium is generated by a cell-counting factor modulating cell-cell adhesion."
    Roisin-Bouffay C., Jang W., Caprette D.R., Gomer R.H.
    Mol. Cell 6:953-959(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Two cell-counting factors regulate the aggregate size of the cellular slime mold Dictyostelium discoideum."
    Okuwa T., Katayama T., Takano A., Kodaira K., Yasukawa H.
    Dev. Growth Differ. 43:735-744(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  6. "The different components of a multisubunit cell number-counting factor have both unique and overlapping functions."
    Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Ammann R., Gomer R.H.
    Development 129:3657-3668(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CF COMPLEX.
  7. "CF45-1, a secreted protein which participates in Dictyostelium group size regulation."
    Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Jang W., Ammann R., Gomer R.H.
    Eukaryot. Cell 2:788-797(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CF COMPLEX.
  8. "A 60-kilodalton protein component of the counting factor complex regulates group size in Dictyostelium discoideum."
    Brock D.A., van Egmond W.N., Shamoo Y., Hatton R.D., Gomer R.H.
    Eukaryot. Cell 5:1532-1538(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CF COMPLEX.

Entry informationi

Entry nameiCTNA_DICDI
AccessioniPrimary (citable) accession number: Q86IV5
Secondary accession number(s): Q556C3, Q9U7C4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3