Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q86IV5 (CTNA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Countin-1
Gene names
Name:ctnA
ORF Names:DDB_G0274597
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length258 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell-counting factor that limits the maximum size of the multicellular structure. May down-regulate the expression of gp24, which mediates cell adhesion. Ref.1 Ref.4 Ref.5

Subunit structure

Component of the counting factor (CF) complex, which includes cf60, cf50, cf45-1 and ctnA. Ref.1 Ref.6 Ref.7 Ref.8

Subcellular location

Secreted Ref.1.

Developmental stage

Highly expressed in the vegetative cells and decreases during subsequent developmental stages (6-24 hours). Ref.1 Ref.5

Disruption phenotype

Cells form a fewer number of larger aggregates. Ref.1

Sequence similarities

Belongs to the countin family.

Contains 1 saposin B-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Ref.1
Chain22 – 258237Countin-1
PRO_0000327908

Regions

Domain22 – 10685Saposin B-type

Amino acid modifications

Glycosylation1211N-linked (GlcNAc...) Potential
Glycosylation2151N-linked (GlcNAc...) Potential
Disulfide bond25 ↔ 102 By similarity
Disulfide bond28 ↔ 96 By similarity
Disulfide bond56 ↔ 69 By similarity

Experimental info

Sequence conflict451Missing in AAD53482. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q86IV5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 725F37870E78356A

FASTA25826,659
        10         20         30         40         50         60 
MNKLFSLILA LFLVNSAVVS SLDSCSICVD FVGNSLNDLL NIILNSGVIG TCGDLCSAVP 

        70         80         90        100        110        120 
GGQIVDTVCD LLCDYVGVDE FIKLISDVDP DPIYICEKIS VCKTNDNAAA SLDLVQINPQ 

       130        140        150        160        170        180 
NGTVGGTFTL SIAYTVTNTI ATGQLAFNII DPTGNAFGDA VLLVQQSPEQ YTQQFQFQAT 

       190        200        210        220        230        240 
PSEQESFPNG LYTVQALVCE GSCGSPHPNT YTLANGTTTF TISGADSSSM SGAGSFSGSS 

       250 
QSTQTGAASG SGSGFALF 

« Hide

References

« Hide 'large scale' references
[1]"A cell-counting factor regulating structure size in Dictyostelium."
Brock D.A., Gomer R.H.
Genes Dev. 13:1960-1969(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-41, IDENTIFICATION IN THE CF COMPLEX, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, FUNCTION, DISRUPTION PHENOTYPE.
[2]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[4]"A precise group size in Dictyostelium is generated by a cell-counting factor modulating cell-cell adhesion."
Roisin-Bouffay C., Jang W., Caprette D.R., Gomer R.H.
Mol. Cell 6:953-959(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Two cell-counting factors regulate the aggregate size of the cellular slime mold Dictyostelium discoideum."
Okuwa T., Katayama T., Takano A., Kodaira K., Yasukawa H.
Dev. Growth Differ. 43:735-744(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[6]"The different components of a multisubunit cell number-counting factor have both unique and overlapping functions."
Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Ammann R., Gomer R.H.
Development 129:3657-3668(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CF COMPLEX.
[7]"CF45-1, a secreted protein which participates in Dictyostelium group size regulation."
Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Jang W., Ammann R., Gomer R.H.
Eukaryot. Cell 2:788-797(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CF COMPLEX.
[8]"A 60-kilodalton protein component of the counting factor complex regulates group size in Dictyostelium discoideum."
Brock D.A., van Egmond W.N., Shamoo Y., Hatton R.D., Gomer R.H.
Eukaryot. Cell 5:1532-1538(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CF COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF140780 Genomic DNA. Translation: AAD53482.1.
AAFI02000012 Genomic DNA. Translation: EAL70191.1.
RefSeqXP_643887.1. XM_638795.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0185089.

Protein family/group databases

TCDB1.C.35.4.1. the amoebapore (amoebapore) family.

Proteomic databases

PRIDEQ86IV5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0185089; DDB0185089; DDB_G0274597.
GeneID8619313.
KEGGddi:DDB_G0274597.

Organism-specific databases

dictyBaseDDB_G0274597. ctnA.

Phylogenomic databases

eggNOGNOG12793.
OMACNGECGS.
PhylomeDBQ86IV5.

Family and domain databases

InterProIPR008139. SaposinB.
[Graphical view]
SMARTSM00741. SapB. 1 hit.
[Graphical view]
PROSITEPS50015. SAP_B. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCTNA_DICDI
AccessionPrimary (citable) accession number: Q86IV5
Secondary accession number(s): Q556C3, Q9U7C4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase