Q86IV5 (CTNA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Countin-1

Gene names

Name:	ctnA
ORF Names:	DDB_G0274597

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cell-counting factor that limits the maximum size of the multicellular structure. May down-regulate the expression of gp24, which mediates cell adhesion. Ref.1 Ref.4 Ref.5
Subunit structure	Component of the counting factor (CF) complex, which includes cf60, cf50, cf45-1 and ctnA. Ref.1 Ref.6 Ref.7 Ref.8
Subcellular location	Secreted Ref.1.
Developmental stage	Highly expressed in the vegetative cells and decreases during subsequent developmental stages (6-24 hours). Ref.1 Ref.5
Disruption phenotype	Cells form a fewer number of larger aggregates. Ref.1
Sequence similarities	Belongs to the countin family. Contains 1 saposin B-type domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	actin filament polymerization Inferred from direct assay PubMed 12070154. Source: dictyBase cellular component movement Inferred from direct assay PubMed 12070154. Source: dictyBase glucose homeostasis Inferred from mutant phenotype Ref.7. Source: dictyBase multicellular organismal development Inferred from mutant phenotype Ref.1. Source: dictyBase negative regulation of aggregate size involved in sorocarp development Inferred from mutant phenotype Ref.1. Source: dictyBase negative regulation of cGMP biosynthetic process Inferred from mutant phenotype PubMed 11371560. Source: dictyBase negative regulation of cell adhesion Inferred from direct assay PubMed 12070154. Source: dictyBase regulation of glucose-6-phosphatase activity Inferred from mutant phenotype PubMed 16606621. Source: dictyBase regulation of glycolysis Inferred from mutant phenotype PubMed 15643062. Source: dictyBase
Cellular_component	extracellular region Inferred from direct assay Ref.1. Source: dictyBase
Molecular_function	signal transducer activity Inferred from mutant phenotype Ref.1. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.1
	Chain	22 – 258	237	Countin-1		PRO_0000327908
Regions
	Domain	22 – 106	85	Saposin B-type
Amino acid modifications
	Glycosylation	121	1	N-linked (GlcNAc...) Potential
	Glycosylation	215	1	N-linked (GlcNAc...) Potential
	Disulfide bond	25 ↔ 102		By similarity
	Disulfide bond	28 ↔ 96		By similarity
	Disulfide bond	56 ↔ 69		By similarity
Experimental info
	Sequence conflict	45	1	Missing in AAD53482. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q86IV5 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 725F37870E78356A	FASTA	258	26,659
	10 20 30 40 50 60 MNKLFSLILA LFLVNSAVVS SLDSCSICVD FVGNSLNDLL NIILNSGVIG TCGDLCSAVP 70 80 90 100 110 120 GGQIVDTVCD LLCDYVGVDE FIKLISDVDP DPIYICEKIS VCKTNDNAAA SLDLVQINPQ 130 140 150 160 170 180 NGTVGGTFTL SIAYTVTNTI ATGQLAFNII DPTGNAFGDA VLLVQQSPEQ YTQQFQFQAT 190 200 210 220 230 240 PSEQESFPNG LYTVQALVCE GSCGSPHPNT YTLANGTTTF TISGADSSSM SGAGSFSGSS 250 QSTQTGAASG SGSGFALF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cell-counting factor regulating structure size in Dictyostelium." Brock D.A., Gomer R.H. Genes Dev. 13:1960-1969(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-41, IDENTIFICATION IN THE CF COMPLEX, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, FUNCTION, DISRUPTION PHENOTYPE.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"A precise group size in Dictyostelium is generated by a cell-counting factor modulating cell-cell adhesion." Roisin-Bouffay C., Jang W., Caprette D.R., Gomer R.H. Mol. Cell 6:953-959(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Two cell-counting factors regulate the aggregate size of the cellular slime mold Dictyostelium discoideum." Okuwa T., Katayama T., Takano A., Kodaira K., Yasukawa H. Dev. Growth Differ. 43:735-744(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[6]	"The different components of a multisubunit cell number-counting factor have both unique and overlapping functions." Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Ammann R., Gomer R.H. Development 129:3657-3668(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CF COMPLEX.
[7]	"CF45-1, a secreted protein which participates in Dictyostelium group size regulation." Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Jang W., Ammann R., Gomer R.H. Eukaryot. Cell 2:788-797(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CF COMPLEX.
[8]	"A 60-kilodalton protein component of the counting factor complex regulates group size in Dictyostelium discoideum." Brock D.A., van Egmond W.N., Shamoo Y., Hatton R.D., Gomer R.H. Eukaryot. Cell 5:1532-1538(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CF COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF140780 Genomic DNA. Translation: AAD53482.1. AAFI02000012 Genomic DNA. Translation: EAL70191.1.
RefSeq	XP_643887.1. XM_638795.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0185089.
Protein family/group databases
TCDB	1.C.35.4.1. amoebapore family.
Proteomic databases
PRIDE	Q86IV5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0185089; DDB0185089; DDB_G0274597.
GeneID	8619313.
KEGG	ddi:DDB_G0274597.
Organism-specific databases
dictyBase	DDB_G0274597. ctnA.
Phylogenomic databases
eggNOG	NOG12793.
OMA	CNGECGS.
ProtClustDB	CLSZ2430664.
Family and domain databases
InterPro	IPR011001. Saposin-like. IPR008139. SaposinB. [Graphical view]
SMART	SM00741. SapB. 1 hit. [Graphical view]
SUPFAM	SSF47862. Saposin_like. 1 hit.
PROSITE	PS50015. SAP_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTNA_DICDI

Accession

Primary (citable) accession number: Q86IV5
Secondary accession number(s): Q556C3, Q9U7C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)