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Q86IV5

- CTNA_DICDI

UniProt

Q86IV5 - CTNA_DICDI

Protein

Countin-1

Gene

ctnA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Cell-counting factor that limits the maximum size of the multicellular structure. May down-regulate the expression of gp24, which mediates cell adhesion.3 Publications

    GO - Molecular functioni

    1. signal transducer activity Source: dictyBase

    GO - Biological processi

    1. actin filament polymerization Source: dictyBase
    2. cellular component movement Source: dictyBase
    3. glucose homeostasis Source: dictyBase
    4. multicellular organismal development Source: dictyBase
    5. negative regulation of aggregate size involved in sorocarp development Source: dictyBase
    6. negative regulation of cell adhesion Source: dictyBase
    7. negative regulation of cGMP biosynthetic process Source: dictyBase
    8. regulation of glucose-6-phosphatase activity Source: dictyBase
    9. regulation of glycolytic process Source: dictyBase
    10. signal transduction Source: GOC

    Protein family/group databases

    TCDBi1.C.35.4.1. the amoebapore (amoebapore) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Countin-1
    Gene namesi
    Name:ctnA
    ORF Names:DDB_G0274597
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0274597. ctnA.

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: dictyBase

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Cells form a fewer number of larger aggregates.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 21211 PublicationAdd
    BLAST
    Chaini22 – 258237Countin-1PRO_0000327908Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi25 ↔ 102PROSITE-ProRule annotation
    Disulfide bondi28 ↔ 96PROSITE-ProRule annotation
    Disulfide bondi56 ↔ 69PROSITE-ProRule annotation
    Glycosylationi121 – 1211N-linked (GlcNAc...)PROSITE-ProRule annotation
    Glycosylationi215 – 2151N-linked (GlcNAc...)PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ86IV5.

    Expressioni

    Developmental stagei

    Highly expressed in the vegetative cells and decreases during subsequent developmental stages (6-24 hours).2 Publications

    Interactioni

    Subunit structurei

    Component of the counting factor (CF) complex, which includes cf60, cf50, cf45-1 and ctnA.4 Publications

    Protein-protein interaction databases

    STRINGi44689.DDB_0185089.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 10685Saposin B-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the countin family.Curated
    Contains 1 saposin B-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG12793.
    OMAiCNGECGS.
    PhylomeDBiQ86IV5.

    Family and domain databases

    InterProiIPR008139. SaposinB.
    [Graphical view]
    SMARTiSM00741. SapB. 1 hit.
    [Graphical view]
    PROSITEiPS50015. SAP_B. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q86IV5-1 [UniParc]FASTAAdd to Basket

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    MNKLFSLILA LFLVNSAVVS SLDSCSICVD FVGNSLNDLL NIILNSGVIG    50
    TCGDLCSAVP GGQIVDTVCD LLCDYVGVDE FIKLISDVDP DPIYICEKIS 100
    VCKTNDNAAA SLDLVQINPQ NGTVGGTFTL SIAYTVTNTI ATGQLAFNII 150
    DPTGNAFGDA VLLVQQSPEQ YTQQFQFQAT PSEQESFPNG LYTVQALVCE 200
    GSCGSPHPNT YTLANGTTTF TISGADSSSM SGAGSFSGSS QSTQTGAASG 250
    SGSGFALF 258
    Length:258
    Mass (Da):26,659
    Last modified:June 1, 2003 - v1
    Checksum:i725F37870E78356A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti45 – 451Missing in AAD53482. (PubMed:10444594)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF140780 Genomic DNA. Translation: AAD53482.1.
    AAFI02000012 Genomic DNA. Translation: EAL70191.1.
    RefSeqiXP_643887.1. XM_638795.1.

    Genome annotation databases

    EnsemblProtistsiDDB0185089; DDB0185089; DDB_G0274597.
    GeneIDi8619313.
    KEGGiddi:DDB_G0274597.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF140780 Genomic DNA. Translation: AAD53482.1 .
    AAFI02000012 Genomic DNA. Translation: EAL70191.1 .
    RefSeqi XP_643887.1. XM_638795.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0185089.

    Protein family/group databases

    TCDBi 1.C.35.4.1. the amoebapore (amoebapore) family.

    Proteomic databases

    PRIDEi Q86IV5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0185089 ; DDB0185089 ; DDB_G0274597 .
    GeneIDi 8619313.
    KEGGi ddi:DDB_G0274597.

    Organism-specific databases

    dictyBasei DDB_G0274597. ctnA.

    Phylogenomic databases

    eggNOGi NOG12793.
    OMAi CNGECGS.
    PhylomeDBi Q86IV5.

    Family and domain databases

    InterProi IPR008139. SaposinB.
    [Graphical view ]
    SMARTi SM00741. SapB. 1 hit.
    [Graphical view ]
    PROSITEi PS50015. SAP_B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A cell-counting factor regulating structure size in Dictyostelium."
      Brock D.A., Gomer R.H.
      Genes Dev. 13:1960-1969(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-41, IDENTIFICATION IN THE CF COMPLEX, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, FUNCTION, DISRUPTION PHENOTYPE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    4. "A precise group size in Dictyostelium is generated by a cell-counting factor modulating cell-cell adhesion."
      Roisin-Bouffay C., Jang W., Caprette D.R., Gomer R.H.
      Mol. Cell 6:953-959(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Two cell-counting factors regulate the aggregate size of the cellular slime mold Dictyostelium discoideum."
      Okuwa T., Katayama T., Takano A., Kodaira K., Yasukawa H.
      Dev. Growth Differ. 43:735-744(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE.
    6. "The different components of a multisubunit cell number-counting factor have both unique and overlapping functions."
      Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Ammann R., Gomer R.H.
      Development 129:3657-3668(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CF COMPLEX.
    7. "CF45-1, a secreted protein which participates in Dictyostelium group size regulation."
      Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Jang W., Ammann R., Gomer R.H.
      Eukaryot. Cell 2:788-797(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CF COMPLEX.
    8. "A 60-kilodalton protein component of the counting factor complex regulates group size in Dictyostelium discoideum."
      Brock D.A., van Egmond W.N., Shamoo Y., Hatton R.D., Gomer R.H.
      Eukaryot. Cell 5:1532-1538(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CF COMPLEX.

    Entry informationi

    Entry nameiCTNA_DICDI
    AccessioniPrimary (citable) accession number: Q86IV5
    Secondary accession number(s): Q556C3, Q9U7C4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3