Reviewed, UniProtKB/Swiss-Prot Q86IV5 (CTNA_DICDI)

Names and origin

Protein names

Recommended name:
    Countin-1

Gene names

Name:	ctnA
ORF Names:	DDB_0185089

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cell-counting factor that limits the maximum size of the multicellular structure. May down-regulate the expression of gp24, which mediates cell adhesion.
Subunit structure	Component of the counting factor (CF) complex, which includes cf60, cf50, cf45-1 and ctnA.
Subcellular location	Secreted.
Developmental stage	Highly expressed in the vegetative cells and decreases during subsequent developmental stages (6-24 hours).
Miscellaneous	Cells lacking cntA form a fewer number of larger aggregates.
Sequence similarities	Belongs to the countin family. Contains 1 saposin B-type domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 21	21
	Chain	22 – 258	237	Countin-1
Regions
	Domain	22 – 106	85	Saposin B-type
Amino acid modifications
	Glycosylation	121	1	N-linked (GlcNAc...) Potential
	Glycosylation	215	1	N-linked (GlcNAc...) Potential
	Disulfide bond	25 ↔ 102		By similarity
	Disulfide bond	28 ↔ 96		By similarity
	Disulfide bond	56 ↔ 69		By similarity
Experimental info
	Sequence conflict	45	1	Missing in AAD53482. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q86IV5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 725F37870E78356A	FASTA	258	26,659
	10 20 30 40 50 60 MNKLFSLILA LFLVNSAVVS SLDSCSICVD FVGNSLNDLL NIILNSGVIG TCGDLCSAVP 70 80 90 100 110 120 GGQIVDTVCD LLCDYVGVDE FIKLISDVDP DPIYICEKIS VCKTNDNAAA SLDLVQINPQ 130 140 150 160 170 180 NGTVGGTFTL SIAYTVTNTI ATGQLAFNII DPTGNAFGDA VLLVQQSPEQ YTQQFQFQAT 190 200 210 220 230 240 PSEQESFPNG LYTVQALVCE GSCGSPHPNT YTLANGTTTF TISGADSSSM SGAGSFSGSS 250 QSTQTGAASG SGSGFALF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cell-counting factor regulating structure size in Dictyostelium." Brock D.A., Gomer R.H. Genes Dev. 13:1960-1969(1999) [PubMed: 10444594] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-41, IDENTIFICATION IN THE COUNTIN COMPLEX, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, FUNCTION, KNOCKOUT.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"A precise group size in Dictyostelium is generated by a cell-counting factor modulating cell-cell adhesion." Roisin-Bouffay C., Jang W., Caprette D.R., Gomer R.H. Mol. Cell 6:953-959(2000) [PubMed: 11090633] [Abstract] Cited for: FUNCTION.
[5]	"Two cell-counting factors regulate the aggregate size of the cellular slime mold Dictyostelium discoideum." Okuwa T., Katayama T., Takano A., Kodaira K., Yasukawa H. Dev. Growth Differ. 43:735-744(2001) [PubMed: 11737154] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF140780 Genomic DNA. Translation: AAD53482.1. AC116986 Genomic DNA. Translation: AAO51901.1. AAFI02000012 Genomic DNA. Translation: EAL70191.1.
RefSeq	XP_643887.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3395201.
KEGG	ddi:DDB_0185089.
Organism-specific databases
dictyBase	DDB0185089. ctnA.
Family and domain databases
InterPro	IPR008139. SaposinB. [Graphical view]
SMART	SM00741. SapB. 1 hit. [Graphical view]
PROSITE	PS50015. SAP_B. 1 hit. [Graphical view]
ProDom	Q86IV5. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

CTNA_DICDI

Accession

Primary (citable) accession number: Q86IV5
Secondary accession number(s): Q556C3, Q9U7C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	September 23, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)