Q86IJ1 (PSDE_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
26S proteasome non-ATPase regulatory subunit 14
EC=3.4.19.-

Alternative name(s):
26S proteasome regulatory subunit RPN11
Sks1 multidrug resistance protein homolog

Gene names

Name:	psmD14
Synonyms:	sks1
ORF Names:	DDB_G0272566

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear By similarity.
Subunit structure	Component of the 19S regulatory cap of the 26S proteasome By similarity.
Sequence similarities	Belongs to the peptidase M67A family. PSMD14 subfamily. Contains 1 MPN (JAB/Mov34) domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Proteasome
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	phagocytic vesicle Inferred from direct assay. Source: dictyBase proteasome complex Inferred from sequence or structural similarity. Source: dictyBase
Molecular function	endopeptidase activity Inferred from sequence or structural similarity. Source: dictyBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	26S proteasome non-ATPase regulatory subunit 14		PRO_0000315595
Regions
	Domain	26 – 139	114	MPN
	Motif	113 – 126	14	JAMM motif
Sites
	Metal binding	113	1	Zinc; catalytic By similarity
	Metal binding	115	1	Zinc; catalytic By similarity
	Metal binding	126	1	Zinc; catalytic By similarity
Experimental info
	Sequence conflict	193 – 194	2	LN → SI in AAB57823. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q86IJ1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 53FA4B37CECD86EE	FASTA	306	34,451
	10 20 30 40 50 60 MNRSLMSLLG REGLGEKITD ATPLPDTAET IHISSLALLK MLQHARAGVP LEVMGLMLGE 70 80 90 100 110 120 LIDEYTIRVI DVFAMPQSGT SVSVEAIDPV FQTKMLDMLK QTGRDEIVIG WYHSHPGFGC 130 140 150 160 170 180 WLSSVDVNTQ QSFEQLQSRA VAVVVDPLQS VRGKVVIDAF RTIKTSPTAE PRQITSNLGH 190 200 210 220 230 240 LQDPSIQALI HGLNRNYYSI AINYRKNELE QKMLLNLHKK KWTEGLIVDK FDTHEQSNEK 250 260 270 280 290 300 QINNLLELTK QYQKSIQDED KIEPEKKEVS AVGKLDPKRH LISDVHTLMA NNVVRVLTVM LDTVTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the sks1 multidrug resistance gene homolog in Dictyostelium discoideum." De Maria A.C., Gomes S.L. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U96916 Genomic DNA. Translation: AAB57823.1. AAFI02000008 Genomic DNA. Translation: EAL70920.1.
RefSeq	XP_644863.1. XM_639771.1.
3D structure databases
ProteinModelPortal	Q86IJ1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q86IJ1.
Protein family/group databases
MEROPS	M67.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191298; DDB0191298; DDB_G0272566.
GeneID	8618542.
GenomeReviews	Gene locus psmD14 in contig CM000151_GR.
KEGG	ddi:DDB_G0272566.
Organism-specific databases
dictyBase	DDB_G0272566. psmD14.
Phylogenomic databases
eggNOG	KOG1555.
GeneTree	EPrGT00050000005766.
HOGENOM	HBG499867.
OMA	RNYYSIA.
PhylomeDB	Q86IJ1.
ProtClustDB	CLSZ2438686.
Family and domain databases
InterPro	IPR000555. Mov34_MPN_PAD1. [Graphical view]
KO	K03030.
Pfam	PF01398. Mov34. 1 hit. [Graphical view]
SMART	SM00232. JAB_MPN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSDE_DICDI

Accession

Primary (citable) accession number: Q86IJ1
Secondary accession number(s): O00850, Q559F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)