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Q86HX0 (ODPB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial

Short name=PDHE1-B
EC=1.2.4.1
Gene names
Name:pdhB
ORF Names:DDB_G0276417
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.

Catalytic activity

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactor

Thiamine pyrophosphate By similarity.

Subunit structure

Tetramer of 2 alpha and 2 beta subunits By similarity.

Subcellular location

Mitochondrion matrix.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2525Mitochondrion Potential
Chain26 – 356331Pyruvate dehydrogenase E1 component subunit beta, mitochondrial
PRO_0000328626

Sites

Binding site851Thiamine pyrophosphate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q86HX0 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: A259CB6753D1FF3C

FASTA35639,068
        10         20         30         40         50         60 
MLSSILKKIQ PSLLVNFRII TRTYATKEVT VRDAINSALD EELARDEKVF IMGEEVAQYN 

        70         80         90        100        110        120 
GAYKITKGLF DKYGGDRIID TPITEAGFAG IGVGAAMAGT RPIIEFMTFN FAMQAIDHII 

       130        140        150        160        170        180 
NSSAKTHYMS GGKVFNPIVW RGPNGPPTAV GAQHSQCFAA WYGSVPGLKV VAPWSAADHR 

       190        200        210        220        230        240 
GLLKSAIRDD NPVVYLESEL LYNYKFDLSD QEQDKEYLVP IGKAKVEREG KDVTIVGFSR 

       250        260        270        280        290        300 
IVSNCMEAAE ILAKEGISAE VINLRTIRPI DAETIVNSLK KTNKLVTVEE GWAQSGIGAE 

       310        320        330        340        350 
ISALMMEHAF DYLDAPIERI CGADVPMPYA SNLENAAMVQ TQNIVNAAKR VTQRNK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]Bienvenut W.V., Ura S., Insall R.H.
Submitted (JUL-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 134-141; 229-240 AND 255-281, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: AX2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000015 Genomic DNA. Translation: EAL69162.1.
RefSeqXP_643119.1. XM_638027.1.

3D structure databases

ProteinModelPortalQ86HX0.
SMRQ86HX0. Positions 28-350.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0229442.

Proteomic databases

PRIDEQ86HX0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0229442; DDB0229442; DDB_G0276417.
GeneID8620524.
KEGGddi:DDB_G0276417.

Organism-specific databases

dictyBaseDDB_G0276417. pdhB.

Phylogenomic databases

eggNOGCOG0022.
OMADPKVFVM.
PhylomeDBQ86HX0.

Family and domain databases

Gene3D3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProIPR027110. PDHB.
IPR029061. THDP-binding.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR005475. Transketolase-like_Pyr-bd.
IPR005476. Transketolase_C.
[Graphical view]
PANTHERPTHR11624:SF56. PTHR11624:SF56. 1 hit.
PfamPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.
ProtoNetSearch...

Other

PROQ86HX0.

Entry information

Entry nameODPB_DICDI
AccessionPrimary (citable) accession number: Q86HX0
Secondary accession number(s): Q551L1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase