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Q86HL5 (HMCSB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxymethylglutaryl-CoA synthase B

Short name=HMG-CoA synthase B
EC=2.3.3.10
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase B
Gene names
Name:hgsB
ORF Names:DDB_G0274871
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase By similarity.

Catalytic activity

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

Pathway

Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.

Sequence similarities

Belongs to the HMG-CoA synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 468468Hydroxymethylglutaryl-CoA synthase B
PRO_0000340242

Regions

Compositional bias371 – 38414Poly-Asn

Sites

Active site851Proton donor/acceptor By similarity
Active site1191Acyl-thioester intermediate By similarity
Active site2501Proton donor/acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q86HL5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: D44CCE967EF23BC6

FASTA46852,921
        10         20         30         40         50         60 
MKKTKDIGIC AIDIYFPQTY VNQSELKKYD KVSNGKYTIG LGQTNMSFVG DREDIVSMAM 

        70         80         90        100        110        120 
TSVKMMMSKY SIDYQSIGRL EVGTETIIDK SKSVKSSIMS LFQEYGNTSL EGVDTLNACY 

       130        140        150        160        170        180 
GGTNALFNSL QWIESSYWDG RYALVVTGDI AVYSKGAARP TGGAGVVTML IGPNATLIFD 

       190        200        210        220        230        240 
QSLRGTHMEN VNDFYKPDLS SEYPYVDGKL SIECYLRALD KCYLEYKKKF ESINDDNKFS 

       250        260        270        280        290        300 
MDSFDYVCFH SPYNRLVQKS YARLIYNDFL QNPNNPKYQD LLPFKDLSTG KDSYINSKLD 

       310        320        330        340        350        360 
QITLKLSLDD FKTKVNPSTL LSKECGNSYC GSVYSGILSL LSNVNDLNNK KVLVFSYGSG 

       370        380        390        400        410        420 
LAASLFSFRI NNNKNRNNNN NNNNCFFKTT NDIGKISNIK ERLSNRVKVS PEEFTRILDI 

       430        440        450        460 
REKSHQMVGA RTPIDTLDYI SAGTFYLEKI DEKLIRHYKS KPIISSKL 

« Hide

References

[1]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000012 Genomic DNA. Translation: EAL70328.1.
RefSeqXP_644076.1. XM_638984.1.

3D structure databases

ProteinModelPortalQ86HL5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0217522.

Proteomic databases

PRIDEQ86HL5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0217522; DDB0217522; DDB_G0274871.
GeneID8619505.
KEGGddi:DDB_G0274871.

Organism-specific databases

dictyBaseDDB_G0274871. hgsB.

Phylogenomic databases

eggNOGCOG3425.
OMAAIAFLIC.
PhylomeDBQ86HL5.

Enzyme and pathway databases

UniPathwayUPA00058; UER00102.

Family and domain databases

Gene3D3.40.47.10. 1 hit.
InterProIPR013746. HMG_CoA_synt_C.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMSSF53901. SSF53901. 3 hits.
TIGRFAMsTIGR01833. HMG-CoA-S_euk. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHMCSB_DICDI
AccessionPrimary (citable) accession number: Q86HL5
Secondary accession number(s): Q555J1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase