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Q86HL5

- HMCSB_DICDI

UniProt

Q86HL5 - HMCSB_DICDI

Protein

Hydroxymethylglutaryl-CoA synthase B

Gene

hgsB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.By similarity

    Catalytic activityi

    Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Proton donor/acceptorBy similarity
    Active sitei119 – 1191Acyl-thioester intermediateBy similarity
    Active sitei250 – 2501Proton donor/acceptorBy similarity

    GO - Molecular functioni

    1. hydroxymethylglutaryl-CoA synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. isoprenoid biosynthetic process Source: InterPro
    2. sterol biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Enzyme and pathway databases

    ReactomeiREACT_204470. PPARA activates gene expression.
    REACT_205241. Activation of gene expression by SREBF (SREBP).
    REACT_227623. Cholesterol biosynthesis.
    UniPathwayiUPA00058; UER00102.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxymethylglutaryl-CoA synthase B (EC:2.3.3.10)
    Short name:
    HMG-CoA synthase B
    Alternative name(s):
    3-hydroxy-3-methylglutaryl coenzyme A synthase B
    Gene namesi
    Name:hgsB
    ORF Names:DDB_G0274871
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0274871. hgsB.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 468468Hydroxymethylglutaryl-CoA synthase BPRO_0000340242Add
    BLAST

    Proteomic databases

    PRIDEiQ86HL5.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDBDRAFT_0217522.

    Structurei

    3D structure databases

    ProteinModelPortaliQ86HL5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi371 – 38414Poly-AsnAdd
    BLAST

    Sequence similaritiesi

    Belongs to the HMG-CoA synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG3425.
    KOiK01641.
    OMAiAIAFLIC.
    PhylomeDBiQ86HL5.

    Family and domain databases

    Gene3Di3.40.47.10. 1 hit.
    InterProiIPR013746. HMG_CoA_synt_C_dom.
    IPR013528. HMG_CoA_synth_N.
    IPR010122. HMG_CoA_synthase_euk.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF08540. HMG_CoA_synt_C. 1 hit.
    PF01154. HMG_CoA_synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53901. SSF53901. 3 hits.
    TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q86HL5-1 [UniParc]FASTAAdd to Basket

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    MKKTKDIGIC AIDIYFPQTY VNQSELKKYD KVSNGKYTIG LGQTNMSFVG    50
    DREDIVSMAM TSVKMMMSKY SIDYQSIGRL EVGTETIIDK SKSVKSSIMS 100
    LFQEYGNTSL EGVDTLNACY GGTNALFNSL QWIESSYWDG RYALVVTGDI 150
    AVYSKGAARP TGGAGVVTML IGPNATLIFD QSLRGTHMEN VNDFYKPDLS 200
    SEYPYVDGKL SIECYLRALD KCYLEYKKKF ESINDDNKFS MDSFDYVCFH 250
    SPYNRLVQKS YARLIYNDFL QNPNNPKYQD LLPFKDLSTG KDSYINSKLD 300
    QITLKLSLDD FKTKVNPSTL LSKECGNSYC GSVYSGILSL LSNVNDLNNK 350
    KVLVFSYGSG LAASLFSFRI NNNKNRNNNN NNNNCFFKTT NDIGKISNIK 400
    ERLSNRVKVS PEEFTRILDI REKSHQMVGA RTPIDTLDYI SAGTFYLEKI 450
    DEKLIRHYKS KPIISSKL 468
    Length:468
    Mass (Da):52,921
    Last modified:June 1, 2003 - v1
    Checksum:iD44CCE967EF23BC6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000012 Genomic DNA. Translation: EAL70328.1.
    RefSeqiXP_644076.1. XM_638984.1.

    Genome annotation databases

    EnsemblProtistsiDDB0217522; DDB0217522; DDB_G0274871.
    GeneIDi8619505.
    KEGGiddi:DDB_G0274871.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000012 Genomic DNA. Translation: EAL70328.1 .
    RefSeqi XP_644076.1. XM_638984.1.

    3D structure databases

    ProteinModelPortali Q86HL5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDBDRAFT_0217522.

    Proteomic databases

    PRIDEi Q86HL5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0217522 ; DDB0217522 ; DDB_G0274871 .
    GeneIDi 8619505.
    KEGGi ddi:DDB_G0274871.

    Organism-specific databases

    dictyBasei DDB_G0274871. hgsB.

    Phylogenomic databases

    eggNOGi COG3425.
    KOi K01641.
    OMAi AIAFLIC.
    PhylomeDBi Q86HL5.

    Enzyme and pathway databases

    UniPathwayi UPA00058 ; UER00102 .
    Reactomei REACT_204470. PPARA activates gene expression.
    REACT_205241. Activation of gene expression by SREBF (SREBP).
    REACT_227623. Cholesterol biosynthesis.

    Family and domain databases

    Gene3Di 3.40.47.10. 1 hit.
    InterProi IPR013746. HMG_CoA_synt_C_dom.
    IPR013528. HMG_CoA_synth_N.
    IPR010122. HMG_CoA_synthase_euk.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF08540. HMG_CoA_synt_C. 1 hit.
    PF01154. HMG_CoA_synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53901. SSF53901. 3 hits.
    TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiHMCSB_DICDI
    AccessioniPrimary (citable) accession number: Q86HL5
    Secondary accession number(s): Q555J1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3