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Q86HL5

- HMCSB_DICDI

UniProt

Q86HL5 - HMCSB_DICDI

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Protein

Hydroxymethylglutaryl-CoA synthase B

Gene

hgsB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.By similarity

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Proton donor/acceptorBy similarity
Active sitei119 – 1191Acyl-thioester intermediateBy similarity
Active sitei250 – 2501Proton donor/acceptorBy similarity

GO - Molecular functioni

  1. hydroxymethylglutaryl-CoA synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. isoprenoid biosynthetic process Source: InterPro
  2. sterol biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

ReactomeiREACT_204470. PPARA activates gene expression.
REACT_205241. Activation of gene expression by SREBF (SREBP).
REACT_227623. Cholesterol biosynthesis.
UniPathwayiUPA00058; UER00102.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase B (EC:2.3.3.10)
Short name:
HMG-CoA synthase B
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase B
Gene namesi
Name:hgsB
ORF Names:DDB_G0274871
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0274871. hgsB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Hydroxymethylglutaryl-CoA synthase BPRO_0000340242Add
BLAST

Proteomic databases

PRIDEiQ86HL5.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0217522.

Structurei

3D structure databases

ProteinModelPortaliQ86HL5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi371 – 38414Poly-AsnAdd
BLAST

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.Curated

Phylogenomic databases

eggNOGiCOG3425.
InParanoidiQ86HL5.
KOiK01641.
OMAiAIAFLIC.
PhylomeDBiQ86HL5.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q86HL5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKTKDIGIC AIDIYFPQTY VNQSELKKYD KVSNGKYTIG LGQTNMSFVG
60 70 80 90 100
DREDIVSMAM TSVKMMMSKY SIDYQSIGRL EVGTETIIDK SKSVKSSIMS
110 120 130 140 150
LFQEYGNTSL EGVDTLNACY GGTNALFNSL QWIESSYWDG RYALVVTGDI
160 170 180 190 200
AVYSKGAARP TGGAGVVTML IGPNATLIFD QSLRGTHMEN VNDFYKPDLS
210 220 230 240 250
SEYPYVDGKL SIECYLRALD KCYLEYKKKF ESINDDNKFS MDSFDYVCFH
260 270 280 290 300
SPYNRLVQKS YARLIYNDFL QNPNNPKYQD LLPFKDLSTG KDSYINSKLD
310 320 330 340 350
QITLKLSLDD FKTKVNPSTL LSKECGNSYC GSVYSGILSL LSNVNDLNNK
360 370 380 390 400
KVLVFSYGSG LAASLFSFRI NNNKNRNNNN NNNNCFFKTT NDIGKISNIK
410 420 430 440 450
ERLSNRVKVS PEEFTRILDI REKSHQMVGA RTPIDTLDYI SAGTFYLEKI
460
DEKLIRHYKS KPIISSKL
Length:468
Mass (Da):52,921
Last modified:June 1, 2003 - v1
Checksum:iD44CCE967EF23BC6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000012 Genomic DNA. Translation: EAL70328.1.
RefSeqiXP_644076.1. XM_638984.1.

Genome annotation databases

EnsemblProtistsiDDB0217522; DDB0217522; DDB_G0274871.
GeneIDi8619505.
KEGGiddi:DDB_G0274871.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000012 Genomic DNA. Translation: EAL70328.1 .
RefSeqi XP_644076.1. XM_638984.1.

3D structure databases

ProteinModelPortali Q86HL5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0217522.

Proteomic databases

PRIDEi Q86HL5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0217522 ; DDB0217522 ; DDB_G0274871 .
GeneIDi 8619505.
KEGGi ddi:DDB_G0274871.

Organism-specific databases

dictyBasei DDB_G0274871. hgsB.

Phylogenomic databases

eggNOGi COG3425.
InParanoidi Q86HL5.
KOi K01641.
OMAi AIAFLIC.
PhylomeDBi Q86HL5.

Enzyme and pathway databases

UniPathwayi UPA00058 ; UER00102 .
Reactomei REACT_204470. PPARA activates gene expression.
REACT_205241. Activation of gene expression by SREBF (SREBP).
REACT_227623. Cholesterol biosynthesis.

Family and domain databases

Gene3Di 3.40.47.10. 1 hit.
InterProi IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF53901. SSF53901. 3 hits.
TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiHMCSB_DICDI
AccessioniPrimary (citable) accession number: Q86HL5
Secondary accession number(s): Q555J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3