Reviewed, UniProtKB/Swiss-Prot Q86H36 (RPA1_DICDI)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase I subunit rpa1
      Short name=RNA polymerase I subunit A1
    EC=2.7.7.6
Alternative name(s):
    DNA-directed RNA polymerase I subunit A
    DNA-directed RNA polymerase I largest subunit

Gene names

Name:	polr1a
Synonyms:	rpa1
ORF Names:	DDB_G0275759

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1615 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family. Contains 1 C2H2-type zinc finger.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Magnesium Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1615	1615	DNA-directed RNA polymerase I subunit rpa1		PRO_0000330755
Regions
	Zinc finger	65 – 81	17	C2H2-type By similarity
	Region	955 – 967	13	Bridging helix By similarity
Sites
	Metal binding	65	1	Zinc By similarity
	Metal binding	68	1	Zinc By similarity
	Metal binding	75	1	Zinc By similarity
	Metal binding	78	1	Zinc By similarity
	Metal binding	593	1	Magnesium; catalytic By similarity
	Metal binding	595	1	Magnesium; catalytic By similarity
	Metal binding	597	1	Magnesium; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q86H36-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 7B0970C64E5EE891	FASTA	1,615	181,190
	10 20 30 40 50 60 MSMSQSALIK EEISSVSFQF YTSDDVRKLS VKQIVEIESF DMLGGPVPGG LHDPALGPTG 70 80 90 100 110 120 PAERCATCSL DFIECPGHFG HIELAIPCYN PVSFQTIFKL LKNKCFHCHH FKHGKPIINQ 130 140 150 160 170 180 FIEELTLLFK GDVVKARNLR ERNVLLRGYE EKKKGKSNEE GEEVMESDES DSDKMDTDEN 190 200 210 220 230 240 KTNGKIICDG VFNAKEIYSG KATHIEDFKR EVFKEFFGSF SGYAIPCGNC GAYSPRLRNG 250 260 270 280 290 300 DASKSKLFIM PLHDKYATKN SAIKLPTNFS SVKEQTEHGT WFAPWEVLDN MKKLFENEKE 310 320 330 340 350 360 ILDMLLGHLV PIVTNKGGAK KNVGRQFTKE SNVDSLFMRV FPVTPARYRP PNFVNGRRSE 370 380 390 400 410 420 HPQNSHYKGM VKSNKMIRQS IDAGEGKAKF LVNSVCDLQM HVNNMYDNSK SNSNTNEVAN 430 440 450 460 470 480 GIKQILEKKE GLFRKHMMGK RVNYAARTVI SPDISLETNE MGVPQYFAKT LTFPQPVTSF 490 500 510 520 530 540 NYNQLAQAVI NGPEQYPGAN FIEDENGHLI NLSKESQEKR IALSKTLLTS HPHAPRGVNK 550 560 570 580 590 600 KVYRHLLSGD YVLANRQPTL HKPGIMGHRV KVLGKNEKTL RMHYSNCSTY NADFDGDEMN 610 620 630 640 650 660 IHFPQSLLAS AEIREICANN YQYLVPRNGA PLRGLIQDHI LTGVLLTKRD TLFTKADFQA 670 680 690 700 710 720 ILYASCWSVN TKHPIVTPPP CILKPVPLWS GKQLISAALN QLTIGRAPLN LEAPSKIPTK 730 740 750 760 770 780 MWGTKGLEIT RDSHVIIRQN EMVAGILDKG HFGASSYGLV HTCYELYDPD VAGSLLTLLG 790 800 810 820 830 840 RMFTNYLQSR GFTCGVDDLL MKNKEEKFRV ETLKKANEEG YTVAAKFADL SKYDEQKARD 850 860 870 880 890 900 FFSKALQSER EVARLDGLLK KGLNQYTSKV IDTLIPGGQQ KPFPKNNFSL MTVSGAKGSV 910 920 930 940 950 960 VNFSQVSCLL GQQELEGKRV PRMVSGKTLP SFQAYDASAR AGGFVMDRFL TGVRPQDYFF 970 980 990 1000 1010 1020 HCMAGREGLI DTAVKTSRSG YLQRCLIKHL EGLSVQYDNT VRESDGSVIQ FNYGEDSLEI 1030 1040 1050 1060 1070 1080 GKTPYLTKFP IIAENYELFK SQFPMEQLIE QLKNQEVVEY NRSIKTQPEL DPVMSKFNPS 1090 1100 1110 1120 1130 1140 SDLGCVSESF MNQLNHYIES NPQKLIKTTS NRDGKINEKD FRNLMYLYYS RSMVSPGESV 1150 1160 1170 1180 1190 1200 GLLCAQSIGE PSTQMTLNTF HLAGRGEANV TLGIPRLREL IMTATTKPIT PLMEFQINDS 1210 1220 1230 1240 1250 1260 TNKVETEKMA KYLEILKLSD IIKDITVQEY FQDTNRNYDI EIEFIPTLQQ VLSLHRIKEK 1270 1280 1290 1300 1310 1320 QLNKLFGEFN KVIKRQVKSQ GKLKVNNGDI GLGSKVRGSD LVEDDSLTIN DDDAPANDDT 1330 1340 1350 1360 1370 1380 TNNDENTSQQ QPSSQNKKSK SKVITQDDDS VAAKSKNKKK QNVNYEDGEE EAEEKDSDEG 1390 1400 1410 1420 1430 1440 ESEAEESDDK SDVDSDSDEI SNSRSSNSFS DESIEFDQKK LTFTISVASD SKKVLMLGIV 1450 1460 1470 1480 1490 1500 ETEASKFVLK SCKGITRCFV NEKQVGGKTH YSIQSEGVNL SEIFELRDKL KIDEIYTNDI 1510 1520 1530 1540 1550 1560 YAILQKYGVE ACRQAVTSEI SNVFAAYGIS VDKRHLILLG DYMTFEGGYR ALNRIGIENN 1570 1580 1590 1600 1610 TSPFQKMSFE TTFSFLSKAS LMGDYDTVTS PSSRIVLGQI VKNGTGSFTI VAPVK

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC115598 Genomic DNA. Translation: AAO53208.1. AAFI02000013 Genomic DNA. Translation: EAL69630.1.
RefSeq	XP_643519.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q86H36.
Genome annotation databases
GeneID	3394678.
GenomeReviews	Gene locus polr1a in contig CM000151_GR.
KEGG	ddi:DDB_0216292.
Organism-specific databases
dictyBase	DDB_G0275759. polr1a.
Phylogenomic databases
OMA	FTCGMDD.
Family and domain databases
InterPro	IPR015699. DNA-dir_RNA_pol1_lsu. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
Gene3D	G3DSA:2.40.40.30. RNA_pol_A. 1 hit. G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit.
PANTHER	PTHR19376:SF11. RNA_polyI. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPA1_DICDI

Accession

Primary (citable) accession number: Q86H36
Secondary accession number(s): Q553A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)