Reviewed, UniProtKB/Swiss-Prot Q86H28 (PROD_DICDI)

Names and origin

Protein names

Recommended name:
    Proline dehydrogenase, mitochondrial
    EC=1.5.99.8
Alternative name(s):
    Proline oxidase

Gene names

Name:	prodh
ORF Names:	DDB_G0275669

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts proline to delta-1-pyrroline-5-carboxylate By similarity.
Catalytic activity	L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor.
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the proline oxidase family.

Ontologies

Keywords
Biological process	Proline metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	proline dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 572		Proline dehydrogenase, mitochondrial		PRO_0000329318
Regions
	Compositional bias	10 – 140	131	Asn-rich

Sequences

	Sequence		Length	Mass (Da)
	Q86H28-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 3240673498631523	FASTA	572	65,262
	10 20 30 40 50 60 MIKNTVRIIN KNSNTFINIR NNNNNNINSS LKSGFGTIKR FNTTLNHNSS NSNIQTPISI 70 80 90 100 110 120 NSTIINNNNN SNNNNSNNII NNDLNVVKFS TISTPNSILD TLNENHSNQT NNVNNKNYNN 130 140 150 160 170 180 NNNNFEKDDK FGPPNNQNNN KLDLDTSKLY VSKSTGELFF TFMILKVCSI NFISDNSQKF 190 200 210 220 230 240 LNLFEKLGLS KPLNFFIKYS FFKQFCAGET IRETEIFTEK LNKLGIGTIL DYAIEELAGS 250 260 270 280 290 300 SEGFDSVAEN ICETIRVAAK NPTNSFSCVK FTGLVTPSVL EKMNTLVSNV TTNVSELPIE 310 320 330 340 350 360 SFNSPLDFYL NQSSSLMKQG SEPLLTSKDI KEIKEFFNRM DKIFQLCHQR GVPILVDAEQ 370 380 390 400 410 420 SYYQVAIHHL TMSYSIKYNK EKPIIYNTYQ MYLVNGMNVL KQHFELSSSQ KFNFKLGAKI 430 440 450 460 470 480 VRGAYMVTES ERSQRLSTEN PVLPTIQDTH KSYNTALDFL LNQIKSDPNS IGLMIASHNE 490 500 510 520 530 540 DSINLGTKLI KQYKIDPTNP NIQFGQLFGM ADFLSFNLVD QHQRIFKYVP FGPVEEVLPY 550 560 570 LIRRMHENKG FIGSNSDKEL FYLKKEIKRR LF

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000013 Genomic DNA. Translation: EAL69585.1.
RefSeq	XP_643510.1.
3D structure databases
SMR	Q86H28. Positions 146-549.
ModBase	Search...
Protein-protein interaction databases
STRING	Q86H28.
Genome annotation databases
GeneID	8620091.
KEGG	ddi:DDBDRAFT_0167252.
Organism-specific databases
dictyBase	DDB_G0275669. prodh.
Phylogenomic databases
eggNOG	KOG0186.
OMA	SKYMPYG.
PhylomeDB	Q86H28.
Enzyme and pathway databases
BRENDA	1.5.99.8. 424.
Family and domain databases
InterPro	IPR002872. Proline_DH. IPR015659. Proline_oxidase. [Graphical view]
PANTHER	PTHR13914. Proline_oxidase. 1 hit.
Pfam	PF01619. Pro_dh. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROD_DICDI

Accession

Primary (citable) accession number: Q86H28
Secondary accession number(s): Q553B0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)