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Protein

Superoxide-generating NADPH oxidase heavy chain subunit B

Gene

noxB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Critical component of the membrane-bound oxidase that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior (By similarity).By similarity

Cofactori

FADCurated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi268 – 2681Iron (heme axial ligand)By similarity
Metal bindingi282 – 2821Iron (heme axial ligand)By similarity
Metal bindingi364 – 3641Iron (heme axial ligand)By similarity
Metal bindingi377 – 3771Iron (heme axial ligand)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi460 – 4667FADSequence analysis

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: dictyBase
  • heme binding Source: dictyBase
  • metal ion binding Source: UniProtKB-KW
  • superoxide-generating NADPH oxidase activity Source: dictyBase

GO - Biological processi

  • sorocarp development Source: dictyBase
  • sporulation resulting in formation of a cellular spore Source: dictyBase
  • superoxide anion generation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Protein family/group databases

PeroxiBasei5485. DdNOx02.

Names & Taxonomyi

Protein namesi
Recommended name:
Superoxide-generating NADPH oxidase heavy chain subunit B (EC:1.-.-.-)
Alternative name(s):
NADPH oxidase B
Superoxide-generating NADPH oxidase flavocytochrome B
Gene namesi
Name:noxB
ORF Names:DDB_G0287101
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287101. noxB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 184184CytoplasmicSequence analysisAdd
BLAST
Transmembranei185 – 20521HelicalSequence analysisAdd
BLAST
Topological domaini206 – 22924ExtracellularSequence analysisAdd
BLAST
Transmembranei230 – 25021HelicalSequence analysisAdd
BLAST
Topological domaini251 – 26919CytoplasmicSequence analysisAdd
BLAST
Transmembranei270 – 29021HelicalSequence analysisAdd
BLAST
Topological domaini291 – 32434ExtracellularSequence analysisAdd
BLAST
Transmembranei325 – 34521HelicalSequence analysisAdd
BLAST
Topological domaini346 – 35510CytoplasmicSequence analysis
Transmembranei356 – 37621HelicalSequence analysisAdd
BLAST
Topological domaini377 – 38812ExtracellularSequence analysisAdd
BLAST
Transmembranei389 – 40921HelicalSequence analysisAdd
BLAST
Topological domaini410 – 698289CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: dictyBase
  • NADPH oxidase complex Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype on vegetative growth, but prevents formation of fruiting bodies.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 698698Superoxide-generating NADPH oxidase heavy chain subunit BPRO_0000361528Add
BLAST

Proteomic databases

PaxDbiQ86GL4.

Expressioni

Developmental stagei

Expressed during development. Expressed during aggregation and shows a marked decrease in fruiting bodies.1 Publication

Interactioni

Subunit structurei

Composed of a heavy chain and a light chain.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0191445.

Structurei

3D structure databases

ProteinModelPortaliQ86GL4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini225 – 375151Ferric oxidoreductaseAdd
BLAST
Domaini404 – 528125FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi143 – 15816Poly-AsnAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
InParanoidiQ86GL4.
KOiK08008.
OMAiSVHINIV.
PhylomeDBiQ86GL4.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q86GL4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEKKELQQE LELQEFQTPK NQQLEKLQEP NGEISSTGNE TSESGISSPP
60 70 80 90 100
ISQNDNSNNE NESLNITPNK PFVSMQEELQ NLDIENIPIP PTIQTPKIYK
110 120 130 140 150
NTNNLIHSKN NLSLPISLSQ ENIVKLDKVD IESNDQVNSN TDNNNNTNNN
160 170 180 190 200
NNTNNNKNEK IGLRSKIFKS KIFIKIRGWW WHRGISTYIM LFYIALNIGV
210 220 230 240 250
GVHMFYNMYH SDIFKFLGLS FCFSRTAARL INLNSAVILL PVLRNFLSWL
260 270 280 290 300
RGTIVNNYIP IDKHLNFHKL CAFMLFCCTI IHCVGHYISF KKINDDVLKI
310 320 330 340 350
DDGKSVAGDY LNININNFPD EKYLFFKSVP GITGHIMLLI LILIVSSSMW
360 370 380 390 400
RIRRPMFEIF WYVHHLFIPF YILLCFHGYS KILKKDPQSW MWIIAPFILY
410 420 430 440 450
SIERLIRIAR SKKRVILEKA IMHPSKVLEL RMKRDNDNFN FKPGQYLYLN
460 470 480 490 500
CPSIAYHEWH PFTITSAPDD PFISVHINIV GNWTRKLFKL LNPDNKLGLI
510 520 530 540 550
QEDLKSTQNR GKRRILKIDG PFGAPAENFF KYRNLVLIGA GIGVTPFSSI
560 570 580 590 600
LRHLKNQNDK QTNADENHLK INKIYFIWIS RQKNSFQWFT DILAELENDE
610 620 630 640 650
RIDSILEIHI FLTGALELDD YAKIKNAQKC HITNLHSKTL FGRPNFRSIF
660 670 680 690
NQLTQLHQRE KIGVFYCGNK ALGKNIIKNC NKFNGKNNCH LIFHKENF
Length:698
Mass (Da):81,239
Last modified:June 1, 2003 - v1
Checksum:i47DFFD42D2D9BE1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY221173 Genomic DNA. Translation: AAO62421.1.
AAFI02000096 Genomic DNA. Translation: EAL63915.1.
RefSeqiXP_637386.1. XM_632294.1.

Genome annotation databases

EnsemblProtistsiDDB0191445; DDB0191445; DDB_G0287101.
GeneIDi8625916.
KEGGiddi:DDB_G0287101.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY221173 Genomic DNA. Translation: AAO62421.1.
AAFI02000096 Genomic DNA. Translation: EAL63915.1.
RefSeqiXP_637386.1. XM_632294.1.

3D structure databases

ProteinModelPortaliQ86GL4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191445.

Protein family/group databases

PeroxiBasei5485. DdNOx02.

Proteomic databases

PaxDbiQ86GL4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0191445; DDB0191445; DDB_G0287101.
GeneIDi8625916.
KEGGiddi:DDB_G0287101.

Organism-specific databases

dictyBaseiDDB_G0287101. noxB.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
InParanoidiQ86GL4.
KOiK08008.
OMAiSVHINIV.
PhylomeDBiQ86GL4.

Miscellaneous databases

PROiQ86GL4.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNOXB_DICDI
AccessioniPrimary (citable) accession number: Q86GL4
Secondary accession number(s): Q54KY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: June 1, 2003
Last modified: December 9, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.