Q86GC8 (ACES_CULPI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylcholinesterase
Short name=AChE
EC=3.1.1.7

Gene names

Name:

ACHE1

Organism

Culex pipiens (House mosquito)

Taxonomic identifier

7175 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicimorpha › Culicoidea › Culicidae › Culicinae › Culicini › Culex › Culex › Culex pipiens complex

Protein attributes

Sequence length	702 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Rapidly hydrolyzes choline released into the synapse.
Catalytic activity	Acetylcholine + H2O = choline + acetate. UniProtKB P22303
Subcellular location	Cell junction › synapse By similarity. Secreted By similarity. Cell membrane; Peripheral membrane protein By similarity.
Polymorphism	Strain SLAB is susceptible to insecticides while strain SR is resistant. Insensitivity to insecticides results from a loss of sensitivity of acetylcholinesterase to organophosphates and carbamates and is due to a variant at position 247. Ref.1
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Biological process	Neurotransmitter degradation
Cellular component	Cell junction Cell membrane Membrane Secreted Synapse
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Hydrolase Serine esterase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetylcholinesterase activity Inferred from electronic annotation. Source: EC carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Potential
	Chain	37 – 702	666	Acetylcholinesterase		PRO_0000008602
Sites
	Active site	327	1	Acyl-ester intermediate By similarity UniProtKB P22303
	Active site	453	1	Charge relay system By similarity UniProtKB P22303
	Active site	567	1	Charge relay system By similarity UniProtKB P22303
Amino acid modifications
	Glycosylation	187	1	N-linked (GlcNAc...) Potential
	Glycosylation	637	1	N-linked (GlcNAc...) Potential
	Disulfide bond	195 ↔ 222		By similarity UniProtKB P22303
	Disulfide bond	381 ↔ 394		By similarity UniProtKB P22303
	Disulfide bond	529 ↔ 650		By similarity UniProtKB P22303
Natural variations
	Natural variant	247	1	G → S in strain: SR; confers resistance to insecticides. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q86GC8 [UniParc]. Last modified August 22, 2003. Version 2. Checksum: 4B11ABF5EA824A07	FASTA	702	78,179
	10 20 30 40 50 60 MEIRGLITRL LGPCHLRHLI LCSLGLYSIL VQSVHCRHHD IGSSVAHQLG SKYSQSSSLS 70 80 90 100 110 120 SSSQSSSSLA EEATLNKDSD AFFTPYIGHG DSVRIVDAEL GTLEREHIHS TTTRRRGLTR 130 140 150 160 170 180 RESSSDATDS DPLVITTDKG KIRGTTLEAP SGKKVDAWMG IPYAQPPLGP LRFRHPRPAE 190 200 210 220 230 240 RWTGVLNATK PPNSCVQIVD TVFGDFPGAT MWNPNTPLSE DCLYINVVVP RPRPKNAAVM 250 260 270 280 290 300 LWIFGGGFYS GTATLDVYDH RTLASEENVI VVSLQYRVAS LGFLFLGTPE APGNAGLFDQ 310 320 330 340 350 360 NLALRWVRDN IHRFGGDPSR VTLFGESAGA VSVSLHLLSA LSRDLFQRAI LQSGSPTAPW 370 380 390 400 410 420 ALVSREEATL RALRLAEAVN CPHDATKLSD AVECLRTKDP NELVDNEWGT LGICEFPFVP 430 440 450 460 470 480 VVDGAFLDET PQRSLASGRF KKTDILTGSN TEEGYYFIIY YLTELLRKEE GVTVTREEFL 490 500 510 520 530 540 QAVRELNPYV NGAARQAIVF EYTDWIEPDN PNSNRDALDK MVGDYHFTCN VNEFAQRYAE 550 560 570 580 590 600 EGNNVFMYLY THRSKGNPWP RWTGVMHGDE INYVFGEPLN SALGYQDDEK DFSRKIMRYW 610 620 630 640 650 660 SNFAKTGNPN PSTPSVDLPE WPKHTAHGRH YLELGLNTTF VGRGPRLRQC AFWKKYLPQL 670 680 690 700 VAATSNLQVT PAPSVPCESS STSYRSTLLL IVTLLLVTRF KI

References

[1]	"Insecticide resistance in mosquito vectors." Weill M., Lutfalla G., Mogensen K., Chandre F., Berthomieu A., Berticat C., Pasteur N., Philips A., Fort P., Raymond M. Nature 423:136-137(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: SLAB and SR.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ515147 mRNA. Translation: CAD56155.1. AJ489456 mRNA. Translation: CAD33707.2.
3D structure databases
ProteinModelPortal	Q86GC8.
ModBase	Search...
Protein family/group databases
MEROPS	S09.979.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
OrthoDB	EOG4X69QJ.
Family and domain databases
InterPro	IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. IPR000997. Cholinesterase. [Graphical view]
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PRINTS	PR00878. CHOLNESTRASE.
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACES_CULPI

Accession

Primary (citable) accession number: Q86GC8
Secondary accession number(s): Q86GD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2003
Last sequence update:	August 22, 2003
Last modified:	April 3, 2013
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)