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Q86G50

- LIPA_TOXGO

UniProt

Q86G50 - LIPA_TOXGO

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Protein
Lipoyl synthase, apicoplast
Gene
lipA
Organism
Toxoplasma gondii
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.1 Publication

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi252 – 2521Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi257 – 2571Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi263 – 2631Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi278 – 2781Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi282 – 2821Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi285 – 2851Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, apicoplast (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Name:lipA
OrganismiToxoplasma gondii
Taxonomic identifieri5811 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaSarcocystidaeToxoplasma

Subcellular locationi

Plastidapicoplast 1 Publication

GO - Cellular componenti

  1. apicoplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apicoplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 6363 Reviewed prediction
Add
BLAST
Chaini64 – 543480Lipoyl synthase, apicoplastUniRule annotation
PRO_0000398234Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ86G50.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q86G50-1 [UniParc]FASTAAdd to Basket

« Hide

MAYFFDFPTD TWVEDASPGG PPKRAFGHGL AAGSSHFASP VSRRRLPTIT    50
ALLLFSLLSA SQSGALSVSQ CSRRVSLGPL LSRVSSVSCT PSAAASALAS 100
SLYPTDSLSS VEGSVAPRPP PSSLAFVLRR VPPAAYSSSL SPSVLRFKHS 150
LPRPLQGSLV CAPGILGGAA GSARFAGCCG SQGRSCGSGK NPELPLKGSK 200
DEVIPRVGTS TAGPRPDWFH VPAPQAASRG AEESRYQQLQ KQIRGLDLHT 250
VCEEAKCPNI GECWNGGTAT LILLGDTCTR GCRFCAIKTS SKPPPPDPLE 300
PEKVADAVAK WDIDYVVMTS VDRDDMPDGG AGHFARTVQL VKKAKPSMLI 350
ECLVSDFQGM EESVRTLAQS GLDVYAHNIE TVRRLTPYVR DKRAKYDQSL 400
RVLHLAKQFN PSLFTKSSIM LGLGETSEEV VRTLRDLRDH DVDVVTLGQY 450
LRPTKQQLGV VEYVTPETFK KYQDIAEEMG FKYVASGPLV RSSYKAGEYY 500
MKHLIDDARK HGRRETVKQV KLEADVGTLK GTTTTFQVNE KEA 543
Length:543
Mass (Da):58,706
Last modified:June 1, 2003 - v1
Checksum:i1DDCD0A1150680E6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ556158 mRNA. Translation: CAD88789.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ556158 mRNA. Translation: CAD88789.1 .

3D structure databases

ProteinModelPortali Q86G50.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Apicomplexan parasites contain a single lipoic acid synthase located in the plastid."
    Thomsen-Zieger N., Schachtner J., Seeber F.
    FEBS Lett. 547:80-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: RH.

Entry informationi

Entry nameiLIPA_TOXGO
AccessioniPrimary (citable) accession number: Q86G50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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