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Q86G50

- LIPA_TOXGO

UniProt

Q86G50 - LIPA_TOXGO

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Protein

Lipoyl synthase, apicoplast

Gene

lipA

Organism
Toxoplasma gondii
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.1 PublicationUniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi252 – 2521Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi257 – 2571Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi263 – 2631Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi278 – 2781Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi282 – 2821Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi285 – 2851Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, apicoplastUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:lipAUniRule annotation
OrganismiToxoplasma gondii
Taxonomic identifieri5811 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaSarcocystidaeToxoplasma

Subcellular locationi

Plastidapicoplast 1 PublicationUniRule annotation

GO - Cellular componenti

  1. apicoplast Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Apicoplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 6363UniRule annotationAdd
BLAST
Chaini64 – 543480Lipoyl synthase, apicoplastPRO_0000398234Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ86G50.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q86G50-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAYFFDFPTD TWVEDASPGG PPKRAFGHGL AAGSSHFASP VSRRRLPTIT
60 70 80 90 100
ALLLFSLLSA SQSGALSVSQ CSRRVSLGPL LSRVSSVSCT PSAAASALAS
110 120 130 140 150
SLYPTDSLSS VEGSVAPRPP PSSLAFVLRR VPPAAYSSSL SPSVLRFKHS
160 170 180 190 200
LPRPLQGSLV CAPGILGGAA GSARFAGCCG SQGRSCGSGK NPELPLKGSK
210 220 230 240 250
DEVIPRVGTS TAGPRPDWFH VPAPQAASRG AEESRYQQLQ KQIRGLDLHT
260 270 280 290 300
VCEEAKCPNI GECWNGGTAT LILLGDTCTR GCRFCAIKTS SKPPPPDPLE
310 320 330 340 350
PEKVADAVAK WDIDYVVMTS VDRDDMPDGG AGHFARTVQL VKKAKPSMLI
360 370 380 390 400
ECLVSDFQGM EESVRTLAQS GLDVYAHNIE TVRRLTPYVR DKRAKYDQSL
410 420 430 440 450
RVLHLAKQFN PSLFTKSSIM LGLGETSEEV VRTLRDLRDH DVDVVTLGQY
460 470 480 490 500
LRPTKQQLGV VEYVTPETFK KYQDIAEEMG FKYVASGPLV RSSYKAGEYY
510 520 530 540
MKHLIDDARK HGRRETVKQV KLEADVGTLK GTTTTFQVNE KEA
Length:543
Mass (Da):58,706
Last modified:June 1, 2003 - v1
Checksum:i1DDCD0A1150680E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ556158 mRNA. Translation: CAD88789.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ556158 mRNA. Translation: CAD88789.1 .

3D structure databases

ProteinModelPortali Q86G50.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Apicomplexan parasites contain a single lipoic acid synthase located in the plastid."
    Thomsen-Zieger N., Schachtner J., Seeber F.
    FEBS Lett. 547:80-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: RH.

Entry informationi

Entry nameiLIPA_TOXGO
AccessioniPrimary (citable) accession number: Q86G50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2003
Last modified: November 26, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3