Reviewed, UniProtKB/Swiss-Prot Q86CS2 (ATG1_DICDI)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase atg1
    EC=2.7.11.1
Alternative name(s):
    Autophagy-related protein 1

Gene names

Name:	atg1
Synonyms:	apg1
ORF Names:	DDB_0185178

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	668 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Serine/threonine protein kinase involved in autophagy. Involved in the control of autophagic vacuolar cell death.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	CytoplasmBy similarity. Preautophagosomal structure membrane; Peripheral membrane proteinBy similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Cytoplasm Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	pre-autophagosomal structure membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 668	668	Serine/threonine-protein kinase atg1		PRO_0000327584
Regions
	Domain	7 – 266	260	Protein kinase
	Nucleotide binding	13 – 21	9	ATP By similarity
	Compositional bias	283 – 301	19	Poly-Asn
	Compositional bias	309 – 348	40	Poly-Asn
	Compositional bias	370 – 386	17	Poly-Asn
	Compositional bias	441 – 470	30	Poly-Gln
Sites
	Active site	135	1	Proton acceptor By similarity
	Binding site	36	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q86CS2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 41B9D9DFD83A2D0C	FASTA	668	77,175
	10 20 30 40 50 60 MKRVGDYILD KRIGWGAFAQ VYKGFSIKTN EPFAIKVVDV CRLADKNSKL TENLNYEIRI 70 80 90 100 110 120 LKELSHTNIV RLYDVLNEET DPTFIYMIME CCEGGDFSKY IRTHKKLTEE KALYFMKQLA 130 140 150 160 170 180 NGLKFLRQKQ IVHRDLKPQN LLLSDDSEHP ILKIGDFGFA KFIDPFSLSD TFCGSPLYMA 190 200 210 220 230 240 PEILHRKNYT VKADLWSVGI ILYEMLVGEP AYNSGSVPDL LNQLQNKKIK LPSHISSDCQ 250 260 270 280 290 300 NLIYSLLQID VEKRISWEDF FNHKWLNLNN NDSYKNNSGN YFNNNNINNN NNNNTNNNNN 310 320 330 340 350 360 NISYPISINS NNTNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNYY NNNNSPPNVY 370 380 390 400 410 420 HASSLPYDFN NNNNNNNSNN YNNNTNVPNS LPFAYNNNIY SSPTEAIPQP TTLNKSKSLE 430 440 450 460 470 480 NTGNTIRAHP FKDDKKSTTI QQPQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ NRQLSNLSTD 490 500 510 520 530 540 FERDLVILDG EELESMERVF NRAVAIAELG DLRQNEPLEC VPLYILALKL MKSKIPNDPS 550 560 570 580 590 600 SSPDKFINTF TEYKRKLVHI FSTSNTSVKN QDHHSSFSPN RFIYENALEF GKKGAVEELY 610 620 630 640 650 660 NNYPTSLQFY TDGTLLLEYL SSIVIDSDDQ EIIKKYLNAF EVRTQICKKN YENSKNTVLN TNSIQNNT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Macroautophagy is required for multicellular development of the social amoeba Dictyostelium discoideum." Otto G.P., Wu M.Y., Kazgan N., Anderson O.R., Kessin R.H. J. Biol. Chem. 278:17636-17645(2003) [PubMed: 12626495] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: AX3 / DH1.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Autophagy gene disruption reveals a non-vacuolar cell death pathway in Dictyostelium." Kosta A., Roisin-Bouffay C., Luciani M.-F., Otto G.P., Kessin R.H., Golstein P. J. Biol. Chem. 279:48404-48409(2004) [PubMed: 15358773] [Abstract] Cited for: FUNCTION.
[4]	"A necrotic cell death model in a protist." Laporte C., Kosta A., Klein G., Aubry L., Lam D., Tresse E., Luciani M.F., Golstein P. Cell Death Differ. 14:266-274(2007) [PubMed: 16810325] [Abstract] Cited for: FUNCTION.
[5]	"Classification and functional annotation of eukaryotic protein kinases." Miranda-Saavedra D., Barton G.J. Proteins 68:893-914(2007) [PubMed: 17557329] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY191011 Genomic DNA. Translation: AAO39074.1. AAFI02000190 Genomic DNA. Translation: EAL61174.1.
RefSeq	XP_629641.1.
3D structure databases
HSSP	HSSP built from PDB template 1OB3 based on UniProtKB Q07785.
ModBase	Search...
Genome annotation databases
GeneID	3386268.
KEGG	ddi:DDB_0185178.
Organism-specific databases
dictyBase	DDB0185178. atg1.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

ATG1_DICDI

Accession

Primary (citable) accession number: Q86CS2
Secondary accession number(s): Q54D55

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	September 23, 2008
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)