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Protein

U5-theraphotoxin-Hs1a 1

Gene
N/A
Organism
Haplopelma schmidti (Chinese bird spider) (Ornithoctonus huwenum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Agglutinates human and mice erythrocytes. This activity can be specifically inhibited by mannosamine. This lectin shows very low toxicity in both mammals and insects.1 Publication

Miscellaneous

Exists in two forms, due to cis-trans isomerization at Gly-79-Pro-80. The trans isomer is the most abundant (75%).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionToxin
LigandLectin

Names & Taxonomyi

Protein namesi
Recommended name:
U5-theraphotoxin-Hs1a 1
Short name:
U5-TRTX-Hs1a
Alternative name(s):
Huwenlectin-1
Huwenlectin-I
SHLP-I
Short name:
SHL-I
OrganismiHaplopelma schmidti (Chinese bird spider) (Ornithoctonus huwenum)
Taxonomic identifieri29017 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeHaplopelma

Organism-specific databases

ArachnoServeriAS000355. U5-theraphotoxin-Hs1a.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000003556222 – 491 PublicationAdd BLAST28
ChainiPRO_000003556350 – 81U5-theraphotoxin-Hs1a 1Add BLAST32

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 631 Publication
Disulfide bondi56 ↔ 681 Publication
Disulfide bondi62 ↔ 751 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi57 – 59Combined sources3
Beta strandi66 – 68Combined sources3
Beta strandi70 – 72Combined sources3
Beta strandi74 – 77Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QK7NMR-A50-81[»]
ProteinModelPortaliQ86C51.
SMRiQ86C51.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ86C51.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the huwentoxin-1 family. Lectin subfamily.Curated

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiView protein in InterPro
IPR011696. Huwentoxin-1.
IPR013140. Huwentoxin_CS1.
PfamiView protein in Pfam
PF07740. Toxin_12. 1 hit.
PROSITEiView protein in PROSITE
PS60021. HWTX_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q86C51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTSMFLTLT GLGLLFVVCY ASESEEKEFP KELLSSIFAA DSDFKVEERG
60 70 80
CLGDKCDYNN GCCSGYVCSR TWKWCVLAGP WRR
Length:83
Mass (Da):9,379
Last modified:June 1, 2003 - v1
Checksum:iCAAF83406599B7C2
GO

Mass spectrometryi

Molecular mass is 3539.9±1 Da from positions 50 - 81. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY263708 mRNA. Translation: AAP33075.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY263708 mRNA. Translation: AAP33075.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QK7NMR-A50-81[»]
ProteinModelPortaliQ86C51.
SMRiQ86C51.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ArachnoServeriAS000355. U5-theraphotoxin-Hs1a.

Miscellaneous databases

EvolutionaryTraceiQ86C51.

Family and domain databases

InterProiView protein in InterPro
IPR011696. Huwentoxin-1.
IPR013140. Huwentoxin_CS1.
PfamiView protein in Pfam
PF07740. Toxin_12. 1 hit.
PROSITEiView protein in PROSITE
PS60021. HWTX_1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTXL1_HAPSC
AccessioniPrimary (citable) accession number: Q86C51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.