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Protein

Glutathione synthetase

Gene

GSS

Organism
Drosophila melanogaster (Fruit fly)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase (Gclc)
  2. Glutathione synthetase, Glutathione synthetase (GSS), Glutathione synthetase, Glutathione synthetase (GSS)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei296SubstrateUniRule annotation1
Binding sitei384ATPUniRule annotation1
Metal bindingi446MagnesiumUniRule annotation1
Binding sitei453ATPUniRule annotation1
Binding sitei504ATPUniRule annotation1
Binding sitei529SubstrateUniRule annotation1
Binding sitei531ATPUniRule annotation1
Binding sitei537ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi442 – 451ATPUniRule annotation10
Nucleotide bindingi475 – 478ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

  • cellular amino acid metabolic process Source: GO_Central
  • cellular response to DNA damage stimulus Source: FlyBase
  • detoxification of arsenic-containing substance Source: FlyBase
  • response to ethanol Source: FlyBase

Keywordsi

Molecular functionLigaseUniRule annotationImported
Biological processGlutathione biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-174403. Glutathione synthesis and recycling.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Short name:
GSH-SUniRule annotation
Gene namesi
Name:GSSImported
ORF Names:CG32495Imported, CG6835Imported, Dmel_CG32495Imported, Dmel_CG6835Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0052495. CG32495.
FBgn0030882. GSS.

Subcellular locationi

GO - Cellular componenti

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

MINTiMINT-1549788.
STRINGi7227.FBpp0290197.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini281 – 381GSH_synthaseInterPro annotationAdd BLAST101

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0021. Eukaryota.
ENOG410XPHH. LUCA.
GeneTreeiENSGT00390000013764.
OMAiFRSDYMV.
OrthoDBiEOG091G05V8.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiView protein in InterPro
IPR004887. Glutathione_synth_subst-bd_euk.
IPR005615. Glutathione_synthase.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiView protein in Pfam
PF03917. GSH_synth_ATP. 2 hits.
PF03199. GSH_synthase. 1 hit.
PIRSFiPIRSF001558. GSHase. 2 hits.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q86B44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDANTAVL RNCIRLPLAE DELLEVTAKA KDYAIMHGAA MRSKTAFSPD
60 70 80 90 100
SLNFAPFVLV PSSFPRKEFE KAVALQPIIN RLMHNVAHDE EFITTTLAET
110 120 130 140 150
IKVDEFTANL FNIYRKVLAH GFTQKTSLGM LRSDLMLESG CPELSPRALR
160 170 180 190 200
TAAGEDRGQD AGAAVGQIAG ANGAAGVGTA AGTGSKEEEH REVQLSRVTK
210 220 230 240 250
EPERRATGAQ SAYCCWKQVE INTIASGFGH LGPASKTIQR FVLSELGHAD
260 270 280 290 300
KLHNMPDNNA LAGLCDGMVK AWDIYAKPQA VILFIIEDVS YNICDQRFHE
310 320 330 340 350
FYIRETYPHI KVLRRTLTEV HREGKLGQSK ELLLGSQEVA VIYFRAGYEP
360 370 380 390 400
GHYHSQADWD ARYLMETSLA IKCPSIHYHL AGTKKVQQAL AQPAVLERFI
410 420 430 440 450
NDPEEIKAVG KIFTGLYSLD DNEAGNASYE MALRTPERFV LKPQREGGGN
460 470 480 490 500
NVYGVDIPDA LKRMSRVERS AWILMDLIHP PLTKGYMVRP GGDMPPQIVD
510 520 530 540 550
MVSELGIFGV VIGDAEHIVH NYQAGHMLRT KLSTANEGGV AAGLGALDSP
560
YLIDSDDEDE QK
Length:562
Mass (Da):61,837
Last modified:November 30, 2010 - v2
Checksum:i82F8B042DA5AE39B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAN09450.2.
AE014298 Genomic DNA. Translation: AAO41699.2.
AE014298 Genomic DNA. Translation: AAO41700.2.
AE014298 Genomic DNA. Translation: AAO41701.2.
EF419254 mRNA. Translation: ABN58449.1.
EF419255 mRNA. Translation: ABN58450.1.
RefSeqiNP_728118.2. NM_167591.2.
NP_788925.2. NM_176752.2.
NP_788926.2. NM_176753.2.
NP_788927.2. NM_176754.2.
UniGeneiDm.23147.
Dm.7230.

Genome annotation databases

EnsemblMetazoaiFBtr0300972; FBpp0290194; FBgn0030882.
FBtr0300973; FBpp0290195; FBgn0030882.
FBtr0300975; FBpp0290197; FBgn0052495.
FBtr0300979; FBpp0290201; FBgn0052495.
GeneIDi318053.
32775.
UCSCiCG32495-RC. d. melanogaster.
CG6835-RC. d. melanogaster.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiQ86B44_DROME
AccessioniPrimary (citable) accession number: Q86B44
Secondary accession number(s): A3FP33, Q8IQZ0
Entry historyiIntegrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: November 30, 2010
Last modified: July 5, 2017
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported