Q86B09 (PMM2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphomannomutase 2
Short name=PMM 2
EC=5.4.2.8

Gene names

Name:	pmmB
ORF Names:	DDB_G0272781

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.
Catalytic activity	Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the eukaryotic PMM family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GDP-mannose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway mannose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphomannomutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Phosphomannomutase 2		PRO_0000327517
Sites
	Active site	12	1	Nucleophile By similarity
	Active site	14	1	By similarity
	Active site	47	1	By similarity
	Active site	189	1	By similarity
	Binding site	21	1	Substrate By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	141	1	Substrate By similarity
	Binding site	179	1	Substrate By similarity
	Binding site	181	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q86B09 [UniParc]. Last modified April 8, 2008. Version 2. Checksum: FBF85BBCBDCC0C0B	FASTA	249	28,769
	10 20 30 40 50 60 MSQNKNTICL FDVDDTLTKP RNAITNEMKE LLASLRTKIK IGVVGGSNFN KIKEQLGENF 70 80 90 100 110 120 INDFDYVFAE NGLIAYKDGS LLEIQDIKKY LGEENIKKFI NFVLHYVADL DIPIKRGTFV 130 140 150 160 170 180 EFRNGMLNIS PIGRNCSQQE REEFEKYDLE HKIRSTMVSI LKEEFKSFGL QYSIGGQISF 190 200 210 220 230 240 DVFPIGWDKT YCLRHLPEDE FKTLYFFGDK TFLGGNDYEI ANHPRITQSF TVKSPANTLA ILNDFFFKK

References

[1]

"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000008 Genomic DNA. Translation: EAS66964.2.
RefSeq	XP_001134630.2. XM_001134630.2.
3D structure databases
ProteinModelPortal	Q86B09.
SMR	Q86B09. Positions 7-246.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0231661.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231661; DDB0231661; DDB_G0272781.
GeneID	8618643.
KEGG	ddi:DDB_G0272781.
Organism-specific databases
dictyBase	DDB_G0272781. pmmB.
Phylogenomic databases
eggNOG	COG0561.
OMA	DKRTIGH.
ProtClustDB	PTZ00174.
Enzyme and pathway databases
UniPathway	UPA00126; UER00424.
Family and domain databases
Gene3D	3.40.50.1000. 2 hits.
InterPro	IPR023214. HAD-like_dom. IPR006379. HAD-SF_hydro_IIB. IPR005002. PMM. [Graphical view]
PANTHER	PTHR10466. PTHR10466. 1 hit.
Pfam	PF03332. PMM. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01484. HAD-SF-IIB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PMM2_DICDI

Accession

Primary (citable) accession number: Q86B09
Secondary accession number(s): Q1ZXM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	May 29, 2013
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)