Reviewed, UniProtKB/Swiss-Prot Q86AV9 (PLDG_DICDI)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol-glycan-specific phospholipase D
      Short name=PI-G PLD
    EC=3.1.4.50
Alternative name(s):
    Glycosyl-phosphatidylinositol-specific phospholipase D
      Short name=GPI-specific phospholipase D
      Short name=GPI-PLD
    Phospholipase G

Gene names

Name:	pldG
ORF Names:	DDB_G0276919

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	948 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane. May also cleave GPI anchor intermediates intracellularly.
Catalytic activity	6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + phosphatidate.
Cofactor	Binds calcium Potential.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the GPLD1 family. Contains 2 FG-GAP repeats.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
Ligand	Calcium
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integrin complex Inferred from electronic annotation. Source: InterPro
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW glycosylphosphatidylinositol phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 948	924	Phosphatidylinositol-glycan-specific phospholipase D		PRO_0000367826
Regions
	Repeat	541 – 579	39	FG-GAP
	Repeat	910 – 947	38	FG-GAP
	Compositional bias	393 – 401	9	Poly-Glu
	Compositional bias	426 – 429	4	Poly-Ser
Amino acid modifications
	Glycosylation	39	1	N-linked (GlcNAc...) Potential
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	148	1	N-linked (GlcNAc...) Potential
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
	Glycosylation	433	1	N-linked (GlcNAc...) Potential
	Glycosylation	452	1	N-linked (GlcNAc...) Potential
	Glycosylation	506	1	N-linked (GlcNAc...) Potential
	Glycosylation	535	1	N-linked (GlcNAc...) Potential
	Glycosylation	749	1	N-linked (GlcNAc...) Potential
	Glycosylation	788	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q86AV9-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4592198420D3F9F2	FASTA	948	105,705
	10 20 30 40 50 60 MKNKIILLWL LLIVILCTIS NVKGCGMTTH NTVARRAYNF SSFDGFEQYQ KYVSENFDVF 70 80 90 100 110 120 DAGAAFPDFG YDCGGLANES EAAHWPPFLR AATKYLLETY PQPWSLDGIR LAVFLLGVTS 130 140 150 160 170 180 HQIADISWHS IGGIQQGLIR AMAGQDFNGT YELAHGNADE GGEFELAYNY DLSWLSDKWY 190 200 210 220 230 240 VPITDIKNIF HSMNYPRVDD ENLLRCNAIL YAGAMGVKIG GRFFYPEIAK KSPFLVDHYQ 250 260 270 280 290 300 DYFIGGLDDM SIWTSYCWPV LMGWMDGEDI GDFCFIQPDP NNDDDNQHLR LHHKHSILKN 310 320 330 340 350 360 GSKIKEALSK SILNEMKITN NGKGVTFSLP NSMEKAINQV LNKFNQNPIG TLLEKYLPNL 370 380 390 400 410 420 FNNKNKFYQE NENEQYNHDE EELNIIDIDI DIEEEEQEEE EDKPIRMLSK NNNFKNYEYL 430 440 450 460 470 480 NDKKKSSSSK LKNKSKKNII KLNSDSNDLG TNFTTIYGQN MYSYFGKDIR SKDLNGDGFD 490 500 510 520 530 540 DLIISSPGFG VPGSMQTGCV YYIISNGSSV TIDGGSGFTS EFDIDQVATG KLCGNETHAK 550 560 570 580 590 600 FGWNIDVLDF NLDGIFDIII GAPSSSNANL QYLGMIYIYL GEKNNPAGEW STESDLPSIT 610 620 630 640 650 660 IQGIEYADTI GTVLRVADCN ADSNADLILG SPHSAGGGTQ RGTVQIFYSS KKRISGIPIS 670 680 690 700 710 720 LNDADYYGHG EVDYEWFGYE IKVAGQGDSS TLLVGSPNYH DEETAIVNIG KITSFPYNVN 730 740 750 760 770 780 LNSFDLNPKF VMVGVNKNDK LGYSYNMVNG SLFGLDNVND IMVLSLPTRG FGDDFDQVGE 790 800 810 820 830 840 VVLIDIDNLS GFVEIKNVNL LLSIKGTTKY SRFGESLLIG KLESTDEFAR LFVGAPLWTD 850 860 870 880 890 900 SIDTGPGCVF TFLPNQHLTN DPAVLKQNII NNTPVVIYDS THSIKTFRID DDSGSSGGRS 910 920 930 940 FNNKRKDSRF GFRILLSDFN NDGKNDLIVS ADRDSSKILE GGSINIFQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Changing patterns of gene expression in Dictyostelium prestalk cell subtypes recognized by in situ hybridization with genes from microarray analyses." Maeda M., Sakamoto H., Iranfar N., Fuller D., Maruo T., Ogihara S., Morio T., Urushihara H., Tanaka Y., Loomis W.F. Eukaryot. Cell 2:627-637(2003) [PubMed: 12796308] [Abstract] Cited for: IDENTIFICATION.
[4]	"A distant evolutionary relationship between GPI-specific phospholipase D and bacterial phosphatidylcholine-preferring phospholipase C." Rigden D.J. FEBS Lett. 569:229-234(2004) [PubMed: 15225639] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC115584 Genomic DNA. Translation: AAO50947.1. AAFI02000019 Genomic DNA. Translation: EAL68961.1.
RefSeq	XP_642807.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q86AV9.
Proteomic databases
PRIDE	Q86AV9.
Genome annotation databases
GeneID	3393964.
GenomeReviews	Gene locus pldG in contig CM000151_GR.
KEGG	ddi:DDB_0229914.
Organism-specific databases
dictyBase	DDB_G0276919. pldG.
Family and domain databases
InterPro	IPR013517. FG-GAP. IPR013519. Int_alpha_beta-p. IPR000413. Integrin_alpha. [Graphical view]
Pfam	PF01839. FG-GAP. 2 hits. [Graphical view]
PRINTS	PR01185. INTEGRINA.
SMART	SM00191. Int_alpha. 6 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLDG_DICDI

Accession

Primary (citable) accession number: Q86AV9
Secondary accession number(s): Q550X5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 1, 2003
Last modified:	October 13, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)