Q86AT9 (DHKI_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hybrid signal transduction histidine kinase I
EC=2.7.13.3

Gene names

Name:	dhkI-1
ORF Names:	DDB_G0273475
AND
Name:	dhkI-2
ORF Names:	DDB_G0295835

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1736 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts as a receptor histidine kinase for a signal transduction pathway. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Post-translational modification	Activation probably requires transfer of a phosphate group between a histidine in the kinase core (transmitter) domain and an aspartate of the receiver domain By similarity.
Sequence similarities	Contains 1 histidine kinase domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain. Contains 1 response regulatory domain.
Caution	The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Ontologies

Keywords
Biological process	Two-component regulatory system
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transducer Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular component movement Inferred from genetic interaction. Source: dictyBase peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component response regulator activity Inferred from electronic annotation. Source: InterPro two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1736	1736	Hybrid signal transduction histidine kinase I		PRO_0000328339
Regions
	Domain	234 – 286	53	PAC
	Domain	287 – 358	72	PAS
	Domain	556 – 908	353	Histidine kinase
	Domain	1551 – 1674	124	Response regulatory
	Compositional bias	420 – 440	21	Poly-Asn
	Compositional bias	444 – 449	6	Poly-Gln
	Compositional bias	453 – 459	7	Poly-Gln
	Compositional bias	474 – 477	4	Poly-Ala
	Compositional bias	766 – 769	4	Poly-Asp
	Compositional bias	989 – 992	4	Poly-Asn
	Compositional bias	995 – 1003	9	Poly-Asn
	Compositional bias	1192 – 1196	5	Poly-Ser
	Compositional bias	1218 – 1224	7	Poly-Ser
	Compositional bias	1241 – 1247	7	Poly-Asn
	Compositional bias	1366 – 1376	11	Poly-Gln
	Compositional bias	1397 – 1405	9	Poly-His
	Compositional bias	1425 – 1454	30	Poly-Asn
	Compositional bias	1486 – 1490	5	Poly-Asn
	Compositional bias	1695 – 1709	15	Poly-Asn
	Compositional bias	1715 – 1720	6	Poly-Asn
Amino acid modifications
	Modified residue	559	1	Phosphohistidine; by autocatalysis By similarity
	Modified residue	1605	1	4-aspartylphosphate By similarity
Experimental info
	Sequence conflict	1559	1	T → P in AAK54090. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q86AT9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 8DEDD5BB99744798	FASTA	1,736	191,865
	10 20 30 40 50 60 MIYLKILEKL NQAIWLFDIN KRVLIWGNQS CKKYKNIESI TSLFNNSNLI NDIRNGKSKH 70 80 90 100 110 120 EHIDIIESNE NGEILKQMIF KYSSIHISDM DARQLYLDTS INLLVNQDSH YLDYEGKRIF 130 140 150 160 170 180 ILVEAIEEIK FIQSPLSSSI ENHNINNNQN NQNSVNINSS NKGQYNRPEP SNMGSWEWNV 190 200 210 220 230 240 QNDTTKASNQ FYKIIGIEND FNKKLNFNDF INKLGIQEIQ PIINNCIESN SSSFEYPLRI 250 260 270 280 290 300 NRKNDNLVRY IQLKGEIIKK DDKVFKVLGV CHDFSEIQEA KDKLEEESKF VEALIGCLKA 310 320 330 340 350 360 GIVACNSNGD LTHFNKSAQD LHGLELNDKT DRKQLLDQIL KCYRSPYEQI NLEKSGTPII 370 380 390 400 410 420 RALSGEFIND QEIIITPTSN QQQSSLSKSN RPRSQSNCSN GNKSQNRLSK NYSTTTTTTN 430 440 450 460 470 480 NNNNNNNNNN NNNNNNNNNN SISQQQQTQV STQQTQQQQN TTNGIGGATT STTAAAATIT 490 500 510 520 530 540 SPQQIPIATK IPTKINDNEF LVLASGQEIV SKDGKTIGAF VALHDITERK RNEVILKNAT 550 560 570 580 590 600 EEAQRANQLK GEFLANISHE LLSPMNSIIG MVGLCLDVAP RNLKEMLNDV VESSKILLDL 610 620 630 640 650 660 LHQILDFTTL ESNSLAVRPF PFKLRDTFNQ LFKVFYTRII EKKISLTFSI DPNIADDFYG 670 680 690 700 710 720 DQNRLKQILS NLIDNAIKFS NSSNINNSIS IIVEQLTHNQ FKKYKDSFRS NSKTHSRLNS 730 740 750 760 770 780 HDDYSIDGDY DDQDNNDSYF GRDINVSDNE SAVGGFSELD EKDNSDDDDE NDDENDETDE 790 800 810 820 830 840 NDDDTDDDTS SNTSRNNISN NLLFHNNTSG GGGGGINKRE KFKKKDREGF VNLKFSIKDR 850 860 870 880 890 900 GIGVPEDKHD FIFDRFFQVD GSYSRVQGGV GLGLSICKKL IEFFGGAIWF ESEASQGSTF 910 920 930 940 950 960 HFILSIKSVE APSPKSPSLQ SSNGSINSTT KLFESSRLKN EIYIPSLPNV QTKKVKKDSN 970 980 990 1000 1010 1020 DNGNNDSTNY GISLSNSMNN ININGSTLNN NNSINNNNNS NDNGPLTSRT PSNSYYNFIA 1030 1040 1050 1060 1070 1080 SLNNTSLKSS SHNTLSSSPT GFNGISNLNI SGLNNLSNLS NNPNNLNNII GNTNGNNNTN 1090 1100 1110 1120 1130 1140 GNNNSGISLN NSNNNIQTPN GLNNSRGSSL ISTPSTKNVK KQHSCPMDLI QRIHSICTPR 1150 1160 1170 1180 1190 1200 GPISKECGLN NLPAYSPPKS PGRSLSHGGK NYSNLISTPR GGVGYSSPPI NSSSSSGGGS 1210 1220 1230 1240 1250 1260 STNGSNSGNS SANNSTPSSS SLSSTPLTSS NTPSPVSISS NNVNNNNQQQ QTQPILSPIL 1270 1280 1290 1300 1310 1320 PLKDNSIDIS DLNNINSLTP NSSNSTSTNV TQSSSNIINN GNSITIINNN PVTPNGKKIV 1330 1340 1350 1360 1370 1380 IVPLLSLQSA SSPKQSQRGY SPKQQYSPKQ YSPKQQYSPK QYSPKQQQQQ QQQQQQLEQL 1390 1400 1410 1420 1430 1440 SGRMSPHKSN LSSTNLHHIH HHHIHQQQSQ QHNNTTVHSN NLILNNNNNN NNNNNNNNNN 1450 1460 1470 1480 1490 1500 NNNNNNNNNN NNNNTTNTSN NHTSDPSKSS NSTPETSPPI SSPRSNNNNN CSLTTIVGVT 1510 1520 1530 1540 1550 1560 TPPQPSSTIT TPQFQSPPIL SGNSGNINLL SSNGTSSGGI EISGSQMIPQ KVLVAEDNTM 1570 1580 1590 1600 1610 1620 NQKLIKTLLT KRGFDITIAK DGKQALDFYH ESKNKSILYD CILMDIQMPI LSGLEATCAI 1630 1640 1650 1660 1670 1680 REIEANEGGH IPIIAVTAHA MKGDKEKFLE SGVDDYVTKP INPKLLYEVI NTQICKYIEE 1690 1700 1710 1720 1730 NRSSSTINEN KNTINNNNNN TNNNNNNNNS SNPVNNNNSN SIDATQQELN NEKIRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The histidine kinases of Dictyostelium." Anjard C., Loomis W.F. (In) Inouye M., Dutta R. (eds.); Histidine kinases in signal transduction, pp.1-1, Academic press, San Diego (2001) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX4.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF362371 Genomic DNA. Translation: AAK54090.1. AAFI02000010 Genomic DNA. Translation: EAL70692.1.
RefSeq	XP_644590.1. XM_639498.1.
3D structure databases
HSSP	HSSP built from PDB template 1OXK based on UniProtKB P39928.
ProteinModelPortal	Q86AT9.
ModBase	Search...
Proteomic databases
PRIDE	Q86AT9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231984; DDB0231984; DDB_G0273475.
GeneID	8618954.
GenomeReviews	Gene locus dhkI-2 in contig CM000151_GR.
KEGG	ddi:DDB_G0273475.
Organism-specific databases
dictyBase	DDB_G0273475. dhkI-1. DDB_G0295835. dhkI-2.
Phylogenomic databases
eggNOG	KOG0519.
GeneTree	EPrGT00050000000304.
ProtClustDB	CLSZ2729225.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011006. CheY-like_superfamily. IPR000014. PAS. IPR000700. PAS-assoc_C. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 2 hits.
Pfam	PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00091. PAS. 1 hit. SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF52172. CheY_like. 1 hit. SSF47384. His_kin_homodim. 1 hit.
PROSITE	PS50109. HIS_KIN. 1 hit. PS50113. PAC. 1 hit. PS50112. PAS. False negative. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHKI_DICDI

Accession

Primary (citable) accession number: Q86AT9
Secondary accession number(s): Q557P4, Q95PH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)