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Q86AS5

- PPCE_DICDI

UniProt

Q86AS5 - PPCE_DICDI

Protein

Prolyl endopeptidase

Gene

prep

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.By similarity

    Catalytic activityi

    Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.

    Enzyme regulationi

    Inhibited by chymostatin, Boc-Glu(NHO-Bz)-Pyrrolidide, Z-Pro-L-prolinal dimethyacetal and the peptide H-H-L-P-P-P-V-OH.1 Publication

    Kineticsi

    1. KM=115 µM for carbobenzoxy-Gly-Pro-p-nitroanilide (at 37 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei609 – 6091Charge relay systemPROSITE-ProRule annotation
    Active sitei693 – 6931Charge relay systemPROSITE-ProRule annotation
    Active sitei730 – 7301Charge relay systemPROSITE-ProRule annotation

    GO - Molecular functioni

    1. oligopeptidase activity Source: dictyBase
    2. serine-type endopeptidase activity Source: InterPro
    3. serine-type exopeptidase activity Source: InterPro

    GO - Biological processi

    1. proteolysis Source: dictyBase
    2. regulation of signal transduction Source: dictyBase

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS09.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prolyl endopeptidase (EC:3.4.21.26)
    Short name:
    PE
    Alternative name(s):
    POase
    Post-proline cleaving enzyme
    Gene namesi
    Name:prep
    Synonyms:dpoA
    ORF Names:DDB_G0274387
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0274387. dpoA.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosol Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Resistance to lithium due to elevation of inositol (1,4,5) trisphosphate.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 760760Prolyl endopeptidasePRO_0000327836Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0185041.

    Structurei

    3D structure databases

    ProteinModelPortaliQ86AS5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S9A family.Curated

    Phylogenomic databases

    eggNOGiCOG1505.
    KOiK01322.
    OMAiDYQTIQI.
    PhylomeDBiQ86AS5.

    Family and domain databases

    Gene3Di2.130.10.120. 1 hit.
    3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR002471. Pept_S9_AS.
    IPR023302. Pept_S9A_N.
    IPR001375. Peptidase_S9.
    IPR002470. Peptidase_S9A.
    [Graphical view]
    PANTHERiPTHR11757. PTHR11757. 1 hit.
    PfamiPF00326. Peptidase_S9. 1 hit.
    PF02897. Peptidase_S9_N. 1 hit.
    [Graphical view]
    PRINTSiPR00862. PROLIGOPTASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q86AS5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFNYPETRR DDSVFDIFKS TEKGSVKVYD PYRHLEDQQS PETKKWVDEE    50
    NKITRSFLDQ DNTSEKISNE IMKMLNFERF DWFRRRGSKL FFSRNPNTLN 100
    QNIIYLIDID QISISKDGKS SAKGFENAIE FLNPNTYSKD GTWSLKSFVI 150
    SKSGDHVCFS YSKAGSDWEE IAVKKIITTN ELKTNKDDEE EKEDLKKKNC 200
    LHYAVVDLPD SINWCKFTSI KWDENETGFI YNRYPKPEKV SDDDKGTETD 250
    TNLNNKVYYH KLGDANESFD RVVFECPENP QWIFGTEFSH DHSSLFISAF 300
    RDCNVEHNLY VIRNFQEAIA NKSAFKVEAL IDNFDACYYY ITNTKQGEYF 350
    FLTNLSAPFN RLISIQLNDD QPIVPNSKSK LEFKEIIPEK DYVLESVSRS 400
    SQEKFYVSYQ KHVQDIIEVY DFNGKYLKDI KLPGPGSASL SATEYHDHIF 450
    INFSNLVSPS VTYYMDSKND ELLLFKEPHI EGFKSSDYEC KQVFYESPKD 500
    KTKIPMFIAY KKTTDITSGN APTYMTGYGG FNISYTQSFS IRNIYFLNKF 550
    NGIFVIANIR GGGEYGKAWH EAGSKKNKQN CFDDFIGAAE YLIKENYTNQ 600
    NKLAVRGGSN GGLLMGAISN QRPDLFKCVV ADVGVMDMLR FHLHTIGSNW 650
    VSDYGRSDNP DDFDVLIKYS PLNNVPKDSN QYPSIMLCTG DHDDRVIPAH 700
    SYKFISELQY QLGKKVDTPL LIRVDKDSGH GAGKGLSKQN NEIADIFNFF 750
    SKVLNVKLNF 760
    Length:760
    Mass (Da):87,555
    Last modified:June 1, 2003 - v1
    Checksum:iF8DD6D07A348F382
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti42 – 421E → R in CAB40787. (PubMed:10329620)Curated
    Sequence conflicti402 – 4021Q → P in CAB40787. (PubMed:10329620)Curated
    Sequence conflicti600 – 6023QNK → PNQ in CAB40787. (PubMed:10329620)Curated
    Sequence conflicti664 – 6641D → E in CAB40787. (PubMed:10329620)Curated
    Sequence conflicti681 – 6811Q → P in CAB40787. (PubMed:10329620)Curated
    Sequence conflicti739 – 7391Q → P in CAB40787. (PubMed:10329620)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ238018 mRNA. Translation: CAB40787.1.
    AAFI02000012 Genomic DNA. Translation: EAL70086.1.
    RefSeqiXP_644295.1. XM_639203.1.

    Genome annotation databases

    EnsemblProtistsiDDB0185041; DDB0185041; DDB_G0274387.
    GeneIDi8619723.
    KEGGiddi:DDB_G0274387.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ238018 mRNA. Translation: CAB40787.1 .
    AAFI02000012 Genomic DNA. Translation: EAL70086.1 .
    RefSeqi XP_644295.1. XM_639203.1.

    3D structure databases

    ProteinModelPortali Q86AS5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0185041.

    Protein family/group databases

    MEROPSi S09.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0185041 ; DDB0185041 ; DDB_G0274387 .
    GeneIDi 8619723.
    KEGGi ddi:DDB_G0274387.

    Organism-specific databases

    dictyBasei DDB_G0274387. dpoA.

    Phylogenomic databases

    eggNOGi COG1505.
    KOi K01322.
    OMAi DYQTIQI.
    PhylomeDBi Q86AS5.

    Miscellaneous databases

    PROi Q86AS5.

    Family and domain databases

    Gene3Di 2.130.10.120. 1 hit.
    3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR002471. Pept_S9_AS.
    IPR023302. Pept_S9A_N.
    IPR001375. Peptidase_S9.
    IPR002470. Peptidase_S9A.
    [Graphical view ]
    PANTHERi PTHR11757. PTHR11757. 1 hit.
    Pfami PF00326. Peptidase_S9. 1 hit.
    PF02897. Peptidase_S9_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00862. PROLIGOPTASE.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00708. PRO_ENDOPEP_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Loss of a prolyl oligopeptidase confers resistance to lithium by elevation of inositol (1,4,5) trisphosphate."
      Williams R.S.B., Eames M., Ryves W.J., Viggars J., Harwood A.J.
      EMBO J. 18:2734-2745(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiPPCE_DICDI
    AccessioniPrimary (citable) accession number: Q86AS5
    Secondary accession number(s): Q554M8, Q9XZR9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3