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Q86AS5 (PPCE_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prolyl endopeptidase

Short name=PE
EC=3.4.21.26
Alternative name(s):
POase
Post-proline cleaving enzyme
Gene names
Name:prep
Synonyms:dpoA
ORF Names:DDB_G0274387
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length760 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long By similarity.

Catalytic activity

Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.

Enzyme regulation

Inhibited by chymostatin, Boc-Glu(NHO-Bz)-Pyrrolidide, Z-Pro-L-prolinal dimethyacetal and the peptide H-H-L-P-P-P-V-OH. Ref.1

Subcellular location

Cytoplasm Ref.1.

Disruption phenotype

Resistance to lithium due to elevation of inositol (1,4,5) trisphosphate. Ref.1

Sequence similarities

Belongs to the peptidase S9A family.

Biophysicochemical properties

Kinetic parameters:

KM=115 µM for carbobenzoxy-Gly-Pro-p-nitroanilide (at 37 degrees Celsius) Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 760760Prolyl endopeptidase
PRO_0000327836

Sites

Active site6091Charge relay system By similarity
Active site6931Charge relay system By similarity
Active site7301Charge relay system By similarity

Experimental info

Sequence conflict421E → R in CAB40787. Ref.1
Sequence conflict4021Q → P in CAB40787. Ref.1
Sequence conflict600 – 6023QNK → PNQ in CAB40787. Ref.1
Sequence conflict6641D → E in CAB40787. Ref.1
Sequence conflict6811Q → P in CAB40787. Ref.1
Sequence conflict7391Q → P in CAB40787. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q86AS5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: F8DD6D07A348F382

FASTA76087,555
        10         20         30         40         50         60 
MKFNYPETRR DDSVFDIFKS TEKGSVKVYD PYRHLEDQQS PETKKWVDEE NKITRSFLDQ 

        70         80         90        100        110        120 
DNTSEKISNE IMKMLNFERF DWFRRRGSKL FFSRNPNTLN QNIIYLIDID QISISKDGKS 

       130        140        150        160        170        180 
SAKGFENAIE FLNPNTYSKD GTWSLKSFVI SKSGDHVCFS YSKAGSDWEE IAVKKIITTN 

       190        200        210        220        230        240 
ELKTNKDDEE EKEDLKKKNC LHYAVVDLPD SINWCKFTSI KWDENETGFI YNRYPKPEKV 

       250        260        270        280        290        300 
SDDDKGTETD TNLNNKVYYH KLGDANESFD RVVFECPENP QWIFGTEFSH DHSSLFISAF 

       310        320        330        340        350        360 
RDCNVEHNLY VIRNFQEAIA NKSAFKVEAL IDNFDACYYY ITNTKQGEYF FLTNLSAPFN 

       370        380        390        400        410        420 
RLISIQLNDD QPIVPNSKSK LEFKEIIPEK DYVLESVSRS SQEKFYVSYQ KHVQDIIEVY 

       430        440        450        460        470        480 
DFNGKYLKDI KLPGPGSASL SATEYHDHIF INFSNLVSPS VTYYMDSKND ELLLFKEPHI 

       490        500        510        520        530        540 
EGFKSSDYEC KQVFYESPKD KTKIPMFIAY KKTTDITSGN APTYMTGYGG FNISYTQSFS 

       550        560        570        580        590        600 
IRNIYFLNKF NGIFVIANIR GGGEYGKAWH EAGSKKNKQN CFDDFIGAAE YLIKENYTNQ 

       610        620        630        640        650        660 
NKLAVRGGSN GGLLMGAISN QRPDLFKCVV ADVGVMDMLR FHLHTIGSNW VSDYGRSDNP 

       670        680        690        700        710        720 
DDFDVLIKYS PLNNVPKDSN QYPSIMLCTG DHDDRVIPAH SYKFISELQY QLGKKVDTPL 

       730        740        750        760 
LIRVDKDSGH GAGKGLSKQN NEIADIFNFF SKVLNVKLNF 

« Hide

References

« Hide 'large scale' references
[1]"Loss of a prolyl oligopeptidase confers resistance to lithium by elevation of inositol (1,4,5) trisphosphate."
Williams R.S.B., Eames M., Ryves W.J., Viggars J., Harwood A.J.
EMBO J. 18:2734-2745(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE.
[2]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ238018 mRNA. Translation: CAB40787.1.
AAFI02000012 Genomic DNA. Translation: EAL70086.1.

3D structure databases

ProteinModelPortalQ86AS5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0185041.

Protein family/group databases

MEROPSS09.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0185041; DDB0185041; DDB_G0274387.
KEGGddi:DDB_G0274387.

Organism-specific databases

dictyBaseDDB_G0274387. dpoA.

Phylogenomic databases

eggNOGCOG1505.
KOK01322.
OMAFRAMENI.
PhylomeDBQ86AS5.
ProtClustDBCLSZ2431049.

Family and domain databases

Gene3D2.130.10.120. 1 hit.
InterProIPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERPTHR11757. PTHR11757. 1 hit.
PfamPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSPR00862. PROLIGOPTASE.
PROSITEPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ86AS5.

Entry information

Entry namePPCE_DICDI
AccessionPrimary (citable) accession number: Q86AS5
Secondary accession number(s): Q554M8, Q9XZR9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase