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Q86AS5

- PPCE_DICDI

UniProt

Q86AS5 - PPCE_DICDI

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Protein
Prolyl endopeptidase
Gene
prep, dpoA, DDB_G0274387
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long By similarity.

Catalytic activityi

Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.

Enzyme regulationi

Inhibited by chymostatin, Boc-Glu(NHO-Bz)-Pyrrolidide, Z-Pro-L-prolinal dimethyacetal and the peptide H-H-L-P-P-P-V-OH.1 Publication

Kineticsi

  1. KM=115 µM for carbobenzoxy-Gly-Pro-p-nitroanilide (at 37 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei609 – 6091Charge relay system By similarity
Active sitei693 – 6931Charge relay system By similarity
Active sitei730 – 7301Charge relay system By similarity

GO - Molecular functioni

  1. oligopeptidase activity Source: dictyBase
  2. serine-type endopeptidase activity Source: InterPro
  3. serine-type exopeptidase activity Source: InterPro

GO - Biological processi

  1. proteolysis Source: dictyBase
  2. regulation of signal transduction Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS09.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl endopeptidase (EC:3.4.21.26)
Short name:
PE
Alternative name(s):
POase
Post-proline cleaving enzyme
Gene namesi
Name:prep
Synonyms:dpoA
ORF Names:DDB_G0274387
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0274387. dpoA.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosol Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Resistance to lithium due to elevation of inositol (1,4,5) trisphosphate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 760760Prolyl endopeptidase
PRO_0000327836Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0185041.

Structurei

3D structure databases

ProteinModelPortaliQ86AS5.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9A family.

Phylogenomic databases

eggNOGiCOG1505.
KOiK01322.
OMAiDYQTIQI.
PhylomeDBiQ86AS5.

Family and domain databases

Gene3Di2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERiPTHR11757. PTHR11757. 1 hit.
PfamiPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSiPR00862. PROLIGOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q86AS5-1 [UniParc]FASTAAdd to Basket

« Hide

MKFNYPETRR DDSVFDIFKS TEKGSVKVYD PYRHLEDQQS PETKKWVDEE    50
NKITRSFLDQ DNTSEKISNE IMKMLNFERF DWFRRRGSKL FFSRNPNTLN 100
QNIIYLIDID QISISKDGKS SAKGFENAIE FLNPNTYSKD GTWSLKSFVI 150
SKSGDHVCFS YSKAGSDWEE IAVKKIITTN ELKTNKDDEE EKEDLKKKNC 200
LHYAVVDLPD SINWCKFTSI KWDENETGFI YNRYPKPEKV SDDDKGTETD 250
TNLNNKVYYH KLGDANESFD RVVFECPENP QWIFGTEFSH DHSSLFISAF 300
RDCNVEHNLY VIRNFQEAIA NKSAFKVEAL IDNFDACYYY ITNTKQGEYF 350
FLTNLSAPFN RLISIQLNDD QPIVPNSKSK LEFKEIIPEK DYVLESVSRS 400
SQEKFYVSYQ KHVQDIIEVY DFNGKYLKDI KLPGPGSASL SATEYHDHIF 450
INFSNLVSPS VTYYMDSKND ELLLFKEPHI EGFKSSDYEC KQVFYESPKD 500
KTKIPMFIAY KKTTDITSGN APTYMTGYGG FNISYTQSFS IRNIYFLNKF 550
NGIFVIANIR GGGEYGKAWH EAGSKKNKQN CFDDFIGAAE YLIKENYTNQ 600
NKLAVRGGSN GGLLMGAISN QRPDLFKCVV ADVGVMDMLR FHLHTIGSNW 650
VSDYGRSDNP DDFDVLIKYS PLNNVPKDSN QYPSIMLCTG DHDDRVIPAH 700
SYKFISELQY QLGKKVDTPL LIRVDKDSGH GAGKGLSKQN NEIADIFNFF 750
SKVLNVKLNF 760
Length:760
Mass (Da):87,555
Last modified:June 1, 2003 - v1
Checksum:iF8DD6D07A348F382
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti42 – 421E → R in CAB40787. 1 Publication
Sequence conflicti402 – 4021Q → P in CAB40787. 1 Publication
Sequence conflicti600 – 6023QNK → PNQ in CAB40787. 1 Publication
Sequence conflicti664 – 6641D → E in CAB40787. 1 Publication
Sequence conflicti681 – 6811Q → P in CAB40787. 1 Publication
Sequence conflicti739 – 7391Q → P in CAB40787. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ238018 mRNA. Translation: CAB40787.1.
AAFI02000012 Genomic DNA. Translation: EAL70086.1.
RefSeqiXP_644295.1. XM_639203.1.

Genome annotation databases

EnsemblProtistsiDDB0185041; DDB0185041; DDB_G0274387.
GeneIDi8619723.
KEGGiddi:DDB_G0274387.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ238018 mRNA. Translation: CAB40787.1 .
AAFI02000012 Genomic DNA. Translation: EAL70086.1 .
RefSeqi XP_644295.1. XM_639203.1.

3D structure databases

ProteinModelPortali Q86AS5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0185041.

Protein family/group databases

MEROPSi S09.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0185041 ; DDB0185041 ; DDB_G0274387 .
GeneIDi 8619723.
KEGGi ddi:DDB_G0274387.

Organism-specific databases

dictyBasei DDB_G0274387. dpoA.

Phylogenomic databases

eggNOGi COG1505.
KOi K01322.
OMAi DYQTIQI.
PhylomeDBi Q86AS5.

Miscellaneous databases

PROi Q86AS5.

Family and domain databases

Gene3Di 2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view ]
PANTHERi PTHR11757. PTHR11757. 1 hit.
Pfami PF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view ]
PRINTSi PR00862. PROLIGOPTASE.
SUPFAMi SSF53474. SSF53474. 1 hit.
PROSITEi PS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Loss of a prolyl oligopeptidase confers resistance to lithium by elevation of inositol (1,4,5) trisphosphate."
    Williams R.S.B., Eames M., Ryves W.J., Viggars J., Harwood A.J.
    EMBO J. 18:2734-2745(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPPCE_DICDI
AccessioniPrimary (citable) accession number: Q86AS5
Secondary accession number(s): Q554M8, Q9XZR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: September 3, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi