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Protein

DNA-directed RNA polymerase III subunit rpc1

Gene

polr3a

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671Zinc 1By similarity
Metal bindingi70 – 701Zinc 1By similarity
Metal bindingi77 – 771Zinc 1By similarity
Metal bindingi80 – 801Zinc 1By similarity
Metal bindingi107 – 1071Zinc 2By similarity
Metal bindingi110 – 1101Zinc 2By similarity
Metal bindingi154 – 1541Zinc 2By similarity
Metal bindingi491 – 4911Magnesium; catalyticBy similarity
Metal bindingi493 – 4931Magnesium; catalyticBy similarity
Metal bindingi495 – 4951Magnesium; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-1834949. Cytosolic sensors of pathogen-associated DNA.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit rpc1 (EC:2.7.7.6)
Short name:
RNA polymerase III subunit C1
Alternative name(s):
DNA-directed RNA polymerase III subunit A
Gene namesi
Name:polr3a
Synonyms:rpc1
ORF Names:DDB_G0277199
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0277199. rpc1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14501450DNA-directed RNA polymerase III subunit rpc1PRO_0000330754Add
BLAST

Proteomic databases

PaxDbiQ86AQ5.
PRIDEiQ86AQ5.

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0216293.

Structurei

3D structure databases

ProteinModelPortaliQ86AQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni832 – 84413Bridging helixBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.Curated

Phylogenomic databases

eggNOGiKOG0261. Eukaryota.
COG0086. LUCA.
InParanoidiQ86AQ5.
KOiK03018.
OMAiMLGPETR.
PhylomeDBiQ86AQ5.

Family and domain databases

InterProiIPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q86AQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIELLKEDDA PKKIGHIQFG LLSEDDIVRL SHVQIVNREL FDLVKRKPTP
60 70 80 90 100
YGVLDNKLGT SDKQAMCTTC GLSIVDCVGH FGYIKLQLPV FHIGYLKNIM
110 120 130 140 150
NILQMICKSC STILLNEEKK QYYLRKMRNK KMDNLQRKSL LKKIFLECRK
160 170 180 190 200
TKECLKCGST NGMIKKSGAF KIIHEKYKGK TESLQDYYAL YDDAIKYNPE
210 220 230 240 250
MKSHIKKAQD DLNPLVALNL FKKISYQDIE IMNMDPVIGR PERLILTYML
260 270 280 290 300
VPPVSIRPSV PMDGGSGTNE DDLTMKLSEI LHINEHIRSN VDRAEMSAIM
310 320 330 340 350
EDWDYLQASC AIYINSDVPG LPLQMKPTKA VRGLSQRLKG KTGRFRGNLS
360 370 380 390 400
GKRVDFSGRT VISPDPNLNI DEVAVPQLIA LTMTYPERVT DYNIERLQKY
410 420 430 440 450
VINGPDRHPG ANYIIYADGV KKWLKFGNRE KFAAELKIGD IVERHIIDGD
460 470 480 490 500
IMLFNRQPSL HKLSIMSHKA RVMPWRTLRF NECVCTPYNA DFDGDEMNIH
510 520 530 540 550
LPQTEEARAE ATILMGVTNN LITPRNGEPL VAATQDFLTA SYLISRRDAF
560 570 580 590 600
YERYRFALMC THFADANEHI DLPPPAILKP VELWTGKQIF EVLLRPSVKS
610 620 630 640 650
HVLCNFETRS RTYSKNLYMC PKDGYVYFRN SELMCGSIDK SIIGGGNKNS
660 670 680 690 700
LFHILMRDFS PTIAANCMTR LAKLCARFLG DQGFSIGIPD IKPAEDLDRK
710 720 730 740 750
KREIIETAYK KCAVFLKDYE SGSLQLSSGC SMEQTFEAKM NQTLSQIRDD
760 770 780 790 800
CGKLCVNDLP NYNSPLIMGL CGSKGSNINI AQMICCVGQQ IVNGTRIPNG
810 820 830 840 850
FTNRTTPHFK HFAKNPKSKG FVSNSFYTGM IPTEFFFHTM GGREGLVDTA
860 870 880 890 900
VKTAETGYMQ RRLMKALEDL STHYDYTVRD SIGGIVQFIY GDDGLDPAGM
910 920 930 940 950
EAKDRPVDFL RAMMSVKSTR QCRNEPEMKP FEIRKLVESI IDSSKFEACT
960 970 980 990 1000
DLFKNEIRVF FNGNEKTKGY IQELISLRKQ FKLSSFDLND NEDEIIIEDN
1010 1020 1030 1040 1050
SPMDISTTTT TTTTTTTNNA VKITNITTDI EMNESNKEEN DKIEKERIEK
1060 1070 1080 1090 1100
EKESKRLKLL GERFANEHVV NQIHRITKTQ IELFLDICLD KYSRARIEPG
1110 1120 1130 1140 1150
TAVGAIGAQS IGEPGTQMTL KTFHFAGVAS MNVTLGVPRI KEIINAAKNI
1160 1170 1180 1190 1200
STPIITASLN CDYDIRSARI VAGRIEKTTL GHVATHIKEV VKRAGCYLSI
1210 1220 1230 1240 1250
KIDKNFVDSL QLEINSKTIS QSIASTKGLK LKPEQITTNG DYKLRIVPPA
1260 1270 1280 1290 1300
NIREGSLYYL QFLKNNLPAV IVKGIPTVNR VVISKVDEKQ ERYQLLVEGY
1310 1320 1330 1340 1350
DLRAVMATPG IKGTHTTSNH IMECENTLGI ETARNTIMSE IDMIMTSHGM
1360 1370 1380 1390 1400
SIDIRHVMLL ADLMSFKGEI LGITRFGIAK MKESVLMLAS FEKTTDHLFD
1410 1420 1430 1440 1450
AAVHHRQDDI VGVSECIIMG VVIPLGTGLF KLLRKSNKNN LPKKSLLLQD
Length:1,450
Mass (Da):163,727
Last modified:June 1, 2003 - v1
Checksum:i4F56DE7F372097A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000019 Genomic DNA. Translation: EAL68785.1.
RefSeqiXP_642724.1. XM_637632.1.

Genome annotation databases

EnsemblProtistsiDDB0216293; DDB0216293; DDB_G0277199.
GeneIDi8620917.
KEGGiddi:DDB_G0277199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000019 Genomic DNA. Translation: EAL68785.1.
RefSeqiXP_642724.1. XM_637632.1.

3D structure databases

ProteinModelPortaliQ86AQ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0216293.

Proteomic databases

PaxDbiQ86AQ5.
PRIDEiQ86AQ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0216293; DDB0216293; DDB_G0277199.
GeneIDi8620917.
KEGGiddi:DDB_G0277199.

Organism-specific databases

dictyBaseiDDB_G0277199. rpc1.

Phylogenomic databases

eggNOGiKOG0261. Eukaryota.
COG0086. LUCA.
InParanoidiQ86AQ5.
KOiK03018.
OMAiMLGPETR.
PhylomeDBiQ86AQ5.

Enzyme and pathway databases

ReactomeiR-DDI-1834949. Cytosolic sensors of pathogen-associated DNA.

Miscellaneous databases

PROiQ86AQ5.

Family and domain databases

InterProiIPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPC1_DICDI
AccessioniPrimary (citable) accession number: Q86AQ5
Secondary accession number(s): Q54ZZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.