Reviewed, UniProtKB/Swiss-Prot Q86AQ5 (RPC1_DICDI)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase III subunit rpc1
      Short name=RNA polymerase III subunit C1
    EC=2.7.7.6
Alternative name(s):
    DNA-directed RNA polymerase III subunit A

Gene names

Name:	polr3a
Synonyms:	rpc1
ORF Names:	DDB_G0277199

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Ligand	Magnesium Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1450	1450	DNA-directed RNA polymerase III subunit rpc1		PRO_0000330754
Regions
	Region	832 – 844	13	Bridging helix By similarity
Sites
	Metal binding	67	1	Zinc 1 By similarity
	Metal binding	70	1	Zinc 1 By similarity
	Metal binding	77	1	Zinc 1 By similarity
	Metal binding	80	1	Zinc 1 By similarity
	Metal binding	107	1	Zinc 2 By similarity
	Metal binding	110	1	Zinc 2 By similarity
	Metal binding	154	1	Zinc 2 By similarity
	Metal binding	491	1	Magnesium; catalytic By similarity
	Metal binding	493	1	Magnesium; catalytic By similarity
	Metal binding	495	1	Magnesium; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q86AQ5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4F56DE7F372097A2	FASTA	1,450	163,727
	10 20 30 40 50 60 MIELLKEDDA PKKIGHIQFG LLSEDDIVRL SHVQIVNREL FDLVKRKPTP YGVLDNKLGT 70 80 90 100 110 120 SDKQAMCTTC GLSIVDCVGH FGYIKLQLPV FHIGYLKNIM NILQMICKSC STILLNEEKK 130 140 150 160 170 180 QYYLRKMRNK KMDNLQRKSL LKKIFLECRK TKECLKCGST NGMIKKSGAF KIIHEKYKGK 190 200 210 220 230 240 TESLQDYYAL YDDAIKYNPE MKSHIKKAQD DLNPLVALNL FKKISYQDIE IMNMDPVIGR 250 260 270 280 290 300 PERLILTYML VPPVSIRPSV PMDGGSGTNE DDLTMKLSEI LHINEHIRSN VDRAEMSAIM 310 320 330 340 350 360 EDWDYLQASC AIYINSDVPG LPLQMKPTKA VRGLSQRLKG KTGRFRGNLS GKRVDFSGRT 370 380 390 400 410 420 VISPDPNLNI DEVAVPQLIA LTMTYPERVT DYNIERLQKY VINGPDRHPG ANYIIYADGV 430 440 450 460 470 480 KKWLKFGNRE KFAAELKIGD IVERHIIDGD IMLFNRQPSL HKLSIMSHKA RVMPWRTLRF 490 500 510 520 530 540 NECVCTPYNA DFDGDEMNIH LPQTEEARAE ATILMGVTNN LITPRNGEPL VAATQDFLTA 550 560 570 580 590 600 SYLISRRDAF YERYRFALMC THFADANEHI DLPPPAILKP VELWTGKQIF EVLLRPSVKS 610 620 630 640 650 660 HVLCNFETRS RTYSKNLYMC PKDGYVYFRN SELMCGSIDK SIIGGGNKNS LFHILMRDFS 670 680 690 700 710 720 PTIAANCMTR LAKLCARFLG DQGFSIGIPD IKPAEDLDRK KREIIETAYK KCAVFLKDYE 730 740 750 760 770 780 SGSLQLSSGC SMEQTFEAKM NQTLSQIRDD CGKLCVNDLP NYNSPLIMGL CGSKGSNINI 790 800 810 820 830 840 AQMICCVGQQ IVNGTRIPNG FTNRTTPHFK HFAKNPKSKG FVSNSFYTGM IPTEFFFHTM 850 860 870 880 890 900 GGREGLVDTA VKTAETGYMQ RRLMKALEDL STHYDYTVRD SIGGIVQFIY GDDGLDPAGM 910 920 930 940 950 960 EAKDRPVDFL RAMMSVKSTR QCRNEPEMKP FEIRKLVESI IDSSKFEACT DLFKNEIRVF 970 980 990 1000 1010 1020 FNGNEKTKGY IQELISLRKQ FKLSSFDLND NEDEIIIEDN SPMDISTTTT TTTTTTTNNA 1030 1040 1050 1060 1070 1080 VKITNITTDI EMNESNKEEN DKIEKERIEK EKESKRLKLL GERFANEHVV NQIHRITKTQ 1090 1100 1110 1120 1130 1140 IELFLDICLD KYSRARIEPG TAVGAIGAQS IGEPGTQMTL KTFHFAGVAS MNVTLGVPRI 1150 1160 1170 1180 1190 1200 KEIINAAKNI STPIITASLN CDYDIRSARI VAGRIEKTTL GHVATHIKEV VKRAGCYLSI 1210 1220 1230 1240 1250 1260 KIDKNFVDSL QLEINSKTIS QSIASTKGLK LKPEQITTNG DYKLRIVPPA NIREGSLYYL 1270 1280 1290 1300 1310 1320 QFLKNNLPAV IVKGIPTVNR VVISKVDEKQ ERYQLLVEGY DLRAVMATPG IKGTHTTSNH 1330 1340 1350 1360 1370 1380 IMECENTLGI ETARNTIMSE IDMIMTSHGM SIDIRHVMLL ADLMSFKGEI LGITRFGIAK 1390 1400 1410 1420 1430 1440 MKESVLMLAS FEKTTDHLFD AAVHHRQDDI VGVSECIIMG VVIPLGTGLF KLLRKSNKNN 1450 LPKKSLLLQD

References

[1]

"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000019 Genomic DNA. Translation: EAL68785.1.
RefSeq	XP_642724.1.
3D structure databases
HSSP	HSSP built from PDB template 1I6H based on UniProtKB P04050.
SMR	Q86AQ5. Positions 12-917.
ModBase	Search...
Protein-protein interaction databases
STRING	Q86AQ5.
Genome annotation databases
GeneID	8620917.
KEGG	ddi:DDB_0216293.
Organism-specific databases
dictyBase	DDB_G0277199. polr3a.
Phylogenomic databases
eggNOG	KOG0261.
HOGENOM	HBG499785.
OMA	LCVNDSY.
PhylomeDB	Q86AQ5.
Family and domain databases
InterPro	IPR015700. DNA-dir_RNA_pol3_lsu. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
Gene3D	G3DSA:2.40.40.30. RNA_pol_A. 1 hit. G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit.
PANTHER	PTHR19376:SF15. RNA_pol3. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPC1_DICDI

Accession

Primary (citable) accession number: Q86AQ5
Secondary accession number(s): Q54ZZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)