Q86AD9 (THIL1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable acetyl-CoA acetyltransferase
EC=2.3.1.9

Alternative name(s):
Acetoacetyl-CoA thiolase

Gene names

ORF Names:

DDB_G0271544

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Ligand	Metal-binding Potassium
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ergosterol biosynthetic process Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	cytosol Inferred from sequence or structural similarity. Source: dictyBase
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from direct assay. Source: dictyBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Probable acetyl-CoA acetyltransferase		PRO_0000327943
Regions
	Region	244 – 246	3	Coenzyme A binding By similarity
Sites
	Active site	110	1	Acyl-thioester intermediate By similarity
	Active site	370	1	Proton acceptor By similarity
	Active site	400	1	Proton acceptor By similarity
	Metal binding	205	1	Potassium By similarity
	Metal binding	266	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	268	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	366	1	Potassium; via carbonyl oxygen By similarity
	Binding site	205	1	Coenzyme A By similarity
	Binding site	249	1	Coenzyme A By similarity
	Binding site	269	1	Coenzyme A By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q86AD9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: B4D9267F4A4590CB	FASTA	414	43,466
	10 20 30 40 50 60 MISNLSKVLN SNVKRMYTTA KNLESVVIVS AVRTPIGSIG GSLSTIPGTK LSSITIEEAV 70 80 90 100 110 120 KRAGIKPSDV DEAIIGNVIS ANLGQAPARQ CALGAGLEQK TITTTINKVC SSGMKAIVFG 130 140 150 160 170 180 AQSIALGHSK TVVAGGFESM SQVPYYADKM RFGAKYGNQT FIDGLVRDGL ADAYNGSAMG 190 200 210 220 230 240 VCGDDCADKY KITREQQDKF AVDSYLRALE AQKNGFFNDE IVQVPIVGRG GKVTYVVEDE 250 260 270 280 290 300 EPKKVLFDKI PNLKPAFTPN GTVTPANASK LNDGASSVIL MSESHAKELG LKPLARIIGY 310 320 330 340 350 360 ADAEQAPIEF PTAPALAIPK ALKNAGINMS QVDLFEINEA FAVVGLANAK ILDIDHNKLN 370 380 390 400 410 VNGGAVALGH PIGSSGCRIV VTLTHLLQNK NLKYGVAAIC NGGGGSTALV LEKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of mitochondrial acetoacetyl-CoA thiolase from Dictyostelium discoideum." Ohmachi T., Tanaka T. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB212872 mRNA. Translation: BAD98242.1. AAFI02000006 Genomic DNA. Translation: EAL71636.1.
RefSeq	XP_645587.1. XM_640495.1.
3D structure databases
HSSP	HSSP built from PDB template 2F2S based on UniProtKB P24752.
ProteinModelPortal	Q86AD9.
SMR	Q86AD9. Positions 22-414.
ModBase	Search...
Protein-protein interaction databases
STRING	Q86AD9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231621; DDB0231621; DDB_G0271544.
GeneID	8618040.
KEGG	ddi:DDB_G0271544.
Organism-specific databases
dictyBase	DDB_G0271544. acat.
Phylogenomic databases
eggNOG	KOG1390.
GeneTree	EPrGT00050000005318.
HOGENOM	HBG370930.
OMA	NPARQCA.
PhylomeDB	Q86AD9.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 4 hits.
KO	K00626.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THIL1_DICDI

Accession

Primary (citable) accession number: Q86AD9
Secondary accession number(s): Q55AV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)