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Q86A85

- PUR1_DICDI

UniProt

Q86A85 - PUR1_DICDI

Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 4Fe-4S cluster per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei22 – 221For GATase activityBy similarity
    Metal bindingi276 – 2761Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi329 – 3291MagnesiumBy similarity
    Metal bindingi391 – 3911MagnesiumBy similarity
    Metal bindingi392 – 3921MagnesiumBy similarity
    Metal bindingi428 – 4281Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi507 – 5071Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi510 – 5101Iron-sulfur (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. amidophosphoribosyltransferase activity Source: UniProtKB
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: InterPro
    5. purine nucleobase metabolic process Source: dictyBase
    6. purine nucleotide biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPAT
    Gene namesi
    Name:purF
    ORF Names:DDB_G0274321
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0274321. purF.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 2121By similarityPRO_0000327801Add
    BLAST
    Chaini22 – 521500AmidophosphoribosyltransferasePRO_0000327802Add
    BLAST

    Proteomic databases

    PRIDEiQ86A85.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0230083.

    Structurei

    3D structure databases

    ProteinModelPortaliQ86A85.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 259238Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi3 – 75Poly-Asn
    Compositional biasi10 – 145Poly-Glu

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0034.
    KOiK00764.
    OMAiACYGCFT.
    PhylomeDBiQ86A85.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q86A85-1 [UniParc]FASTAAdd to Basket

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    MENNNNNFIE EEEELSEPKE KCGVFAIYAP ELDVSRIAFF GLVALQHRGQ    50
    ESCGIATYDE FQSVHVETGM GLVNQVFNET NLKPLKGKMA IGHTRYSTAG 100
    KSTLVNAQPV IVQTLHGQIG IVQNGNLTTA KSLRKELMQK GVGFFIDSDV 150
    EVITQLLSNN PEGCDPHKPN WENRIAHFMS KAEAAYALCL MTPNGIYGVR 200
    DSLGMRPLCL GSLEVPCKDD PTKTITRYVL TSESCAIGTI GAKFIRDVRP 250
    GEIVHINENG ITSFIGRSPS DNPALCVFEY VYFSRPDSSM EGQLIHIVRQ 300
    RMGETLARES PPPQTCSNND TIVIGVPDSS LPAAIGYAKQ SGIPFTEGLT 350
    KNRYIHRTFI QPSDHLRQQG IKLKFNPLTE NIQGKKVILV DDSIVRANTI 400
    KALIKLIRGA GATEIHVRIS SPPVLHPCHM GIDMATYDQL IGHNRTVEEI 450
    REYIGAESLQ YLTLEGLMKS VNIGIKPQAE TNSTPCFSTS SPTTTKIKIN 500
    ENNQKHCVAC FTGDYPCSLD F 521
    Length:521
    Mass (Da):57,429
    Last modified:June 1, 2003 - v1
    Checksum:iF9A53FBB08B63AC3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000012 Genomic DNA. Translation: EAL70053.1.
    RefSeqiXP_643947.1. XM_638855.1.

    Genome annotation databases

    EnsemblProtistsiDDB0230083; DDB0230083; DDB_G0274321.
    GeneIDi8619375.
    KEGGiddi:DDB_G0274321.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000012 Genomic DNA. Translation: EAL70053.1 .
    RefSeqi XP_643947.1. XM_638855.1.

    3D structure databases

    ProteinModelPortali Q86A85.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0230083.

    Protein family/group databases

    MEROPSi C44.001.

    Proteomic databases

    PRIDEi Q86A85.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0230083 ; DDB0230083 ; DDB_G0274321 .
    GeneIDi 8619375.
    KEGGi ddi:DDB_G0274321.

    Organism-specific databases

    dictyBasei DDB_G0274321. purF.

    Phylogenomic databases

    eggNOGi COG0034.
    KOi K00764.
    OMAi ACYGCFT.
    PhylomeDBi Q86A85.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .

    Miscellaneous databases

    PROi Q86A85.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiPUR1_DICDI
    AccessioniPrimary (citable) accession number: Q86A85
    Secondary accession number(s): Q555W3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3