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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Bifunctional purine biosynthetic protein purD (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei22NucleophilePROSITE-ProRule annotation1
Metal bindingi276Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi329MagnesiumBy similarity1
Metal bindingi391MagnesiumBy similarity1
Metal bindingi392MagnesiumBy similarity1
Metal bindingi428Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi507Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi510Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Glycosyltransferase, Transferase
Biological processPurine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-DDI-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00124

Protein family/group databases

MEROPSiC44.001

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name:
GPAT
Gene namesi
Name:purF
ORF Names:DDB_G0274321
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0274321 purF

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00003278011 – 21By similarityAdd BLAST21
ChainiPRO_000032780222 – 521AmidophosphoribosyltransferaseAdd BLAST500

Proteomic databases

PaxDbiQ86A85

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0230083

Structurei

3D structure databases

ProteinModelPortaliQ86A85
SMRiQ86A85
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 259Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST238

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 7Poly-Asn5
Compositional biasi10 – 14Poly-Glu5

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG0572 Eukaryota
COG0034 LUCA
InParanoidiQ86A85
KOiK00764
OMAiFRPLCLG
PhylomeDBiQ86A85

Family and domain databases

CDDicd00715 GPATase_N, 1 hit
cd06223 PRTases_typeI, 1 hit
Gene3Di3.60.20.102 hits
HAMAPiMF_01931 PurF, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005854 PurF
IPR035584 PurF_N
PfamiView protein in Pfam
PF00156 Pribosyltran, 1 hit
PIRSFiPIRSF000485 Amd_phspho_trans, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
SSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01134 purF, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q86A85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENNNNNFIE EEEELSEPKE KCGVFAIYAP ELDVSRIAFF GLVALQHRGQ
60 70 80 90 100
ESCGIATYDE FQSVHVETGM GLVNQVFNET NLKPLKGKMA IGHTRYSTAG
110 120 130 140 150
KSTLVNAQPV IVQTLHGQIG IVQNGNLTTA KSLRKELMQK GVGFFIDSDV
160 170 180 190 200
EVITQLLSNN PEGCDPHKPN WENRIAHFMS KAEAAYALCL MTPNGIYGVR
210 220 230 240 250
DSLGMRPLCL GSLEVPCKDD PTKTITRYVL TSESCAIGTI GAKFIRDVRP
260 270 280 290 300
GEIVHINENG ITSFIGRSPS DNPALCVFEY VYFSRPDSSM EGQLIHIVRQ
310 320 330 340 350
RMGETLARES PPPQTCSNND TIVIGVPDSS LPAAIGYAKQ SGIPFTEGLT
360 370 380 390 400
KNRYIHRTFI QPSDHLRQQG IKLKFNPLTE NIQGKKVILV DDSIVRANTI
410 420 430 440 450
KALIKLIRGA GATEIHVRIS SPPVLHPCHM GIDMATYDQL IGHNRTVEEI
460 470 480 490 500
REYIGAESLQ YLTLEGLMKS VNIGIKPQAE TNSTPCFSTS SPTTTKIKIN
510 520
ENNQKHCVAC FTGDYPCSLD F
Length:521
Mass (Da):57,429
Last modified:June 1, 2003 - v1
Checksum:iF9A53FBB08B63AC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000012 Genomic DNA Translation: EAL70053.1
RefSeqiXP_643947.1, XM_638855.1

Genome annotation databases

EnsemblProtistsiEAL70053; EAL70053; DDB_G0274321
GeneIDi8619375
KEGGiddi:DDB_G0274321

Similar proteinsi

Entry informationi

Entry nameiPUR1_DICDI
AccessioniPrimary (citable) accession number: Q86A85
Secondary accession number(s): Q555W3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome