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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathway:iIMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Bifunctional purine biosynthetic protein purD (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei22 – 221NucleophilePROSITE-ProRule annotation
Metal bindingi276 – 2761Iron-sulfur (4Fe-4S)By similarity
Metal bindingi329 – 3291MagnesiumBy similarity
Metal bindingi391 – 3911MagnesiumBy similarity
Metal bindingi392 – 3921MagnesiumBy similarity
Metal bindingi428 – 4281Iron-sulfur (4Fe-4S)By similarity
Metal bindingi507 – 5071Iron-sulfur (4Fe-4S)By similarity
Metal bindingi510 – 5101Iron-sulfur (4Fe-4S)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_327673. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name:
GPAT
Gene namesi
Name:purF
ORF Names:DDB_G0274321
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0274321. purF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 2121By similarityPRO_0000327801Add
BLAST
Chaini22 – 521500AmidophosphoribosyltransferasePRO_0000327802Add
BLAST

Proteomic databases

PRIDEiQ86A85.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0230083.

Structurei

3D structure databases

ProteinModelPortaliQ86A85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 259238Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 75Poly-Asn
Compositional biasi10 – 145Poly-Glu

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
InParanoidiQ86A85.
KOiK00764.
OMAiDWPSQID.
PhylomeDBiQ86A85.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q86A85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENNNNNFIE EEEELSEPKE KCGVFAIYAP ELDVSRIAFF GLVALQHRGQ
60 70 80 90 100
ESCGIATYDE FQSVHVETGM GLVNQVFNET NLKPLKGKMA IGHTRYSTAG
110 120 130 140 150
KSTLVNAQPV IVQTLHGQIG IVQNGNLTTA KSLRKELMQK GVGFFIDSDV
160 170 180 190 200
EVITQLLSNN PEGCDPHKPN WENRIAHFMS KAEAAYALCL MTPNGIYGVR
210 220 230 240 250
DSLGMRPLCL GSLEVPCKDD PTKTITRYVL TSESCAIGTI GAKFIRDVRP
260 270 280 290 300
GEIVHINENG ITSFIGRSPS DNPALCVFEY VYFSRPDSSM EGQLIHIVRQ
310 320 330 340 350
RMGETLARES PPPQTCSNND TIVIGVPDSS LPAAIGYAKQ SGIPFTEGLT
360 370 380 390 400
KNRYIHRTFI QPSDHLRQQG IKLKFNPLTE NIQGKKVILV DDSIVRANTI
410 420 430 440 450
KALIKLIRGA GATEIHVRIS SPPVLHPCHM GIDMATYDQL IGHNRTVEEI
460 470 480 490 500
REYIGAESLQ YLTLEGLMKS VNIGIKPQAE TNSTPCFSTS SPTTTKIKIN
510 520
ENNQKHCVAC FTGDYPCSLD F
Length:521
Mass (Da):57,429
Last modified:June 1, 2003 - v1
Checksum:iF9A53FBB08B63AC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000012 Genomic DNA. Translation: EAL70053.1.
RefSeqiXP_643947.1. XM_638855.1.

Genome annotation databases

EnsemblProtistsiDDB0230083; DDB0230083; DDB_G0274321.
GeneIDi8619375.
KEGGiddi:DDB_G0274321.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000012 Genomic DNA. Translation: EAL70053.1.
RefSeqiXP_643947.1. XM_638855.1.

3D structure databases

ProteinModelPortaliQ86A85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0230083.

Protein family/group databases

MEROPSiC44.001.

Proteomic databases

PRIDEiQ86A85.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0230083; DDB0230083; DDB_G0274321.
GeneIDi8619375.
KEGGiddi:DDB_G0274321.

Organism-specific databases

dictyBaseiDDB_G0274321. purF.

Phylogenomic databases

eggNOGiCOG0034.
InParanoidiQ86A85.
KOiK00764.
OMAiDWPSQID.
PhylomeDBiQ86A85.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.
ReactomeiREACT_327673. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

PROiQ86A85.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPUR1_DICDI
AccessioniPrimary (citable) accession number: Q86A85
Secondary accession number(s): Q555W3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: July 22, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.