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Q86A85 (PUR1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPAT
Gene names
Name:purF
ORF Names:DDB_G0274321
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length521 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 2121 By similarity
PRO_0000327801
Chain22 – 521500Amidophosphoribosyltransferase
PRO_0000327802

Regions

Domain22 – 259238Glutamine amidotransferase type-2
Compositional bias3 – 75Poly-Asn
Compositional bias10 – 145Poly-Glu

Sites

Active site221For GATase activity By similarity
Metal binding2761Iron-sulfur (4Fe-4S) By similarity
Metal binding3291Magnesium By similarity
Metal binding3911Magnesium By similarity
Metal binding3921Magnesium By similarity
Metal binding4281Iron-sulfur (4Fe-4S) By similarity
Metal binding5071Iron-sulfur (4Fe-4S) By similarity
Metal binding5101Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q86A85 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: F9A53FBB08B63AC3

FASTA52157,429
        10         20         30         40         50         60 
MENNNNNFIE EEEELSEPKE KCGVFAIYAP ELDVSRIAFF GLVALQHRGQ ESCGIATYDE 

        70         80         90        100        110        120 
FQSVHVETGM GLVNQVFNET NLKPLKGKMA IGHTRYSTAG KSTLVNAQPV IVQTLHGQIG 

       130        140        150        160        170        180 
IVQNGNLTTA KSLRKELMQK GVGFFIDSDV EVITQLLSNN PEGCDPHKPN WENRIAHFMS 

       190        200        210        220        230        240 
KAEAAYALCL MTPNGIYGVR DSLGMRPLCL GSLEVPCKDD PTKTITRYVL TSESCAIGTI 

       250        260        270        280        290        300 
GAKFIRDVRP GEIVHINENG ITSFIGRSPS DNPALCVFEY VYFSRPDSSM EGQLIHIVRQ 

       310        320        330        340        350        360 
RMGETLARES PPPQTCSNND TIVIGVPDSS LPAAIGYAKQ SGIPFTEGLT KNRYIHRTFI 

       370        380        390        400        410        420 
QPSDHLRQQG IKLKFNPLTE NIQGKKVILV DDSIVRANTI KALIKLIRGA GATEIHVRIS 

       430        440        450        460        470        480 
SPPVLHPCHM GIDMATYDQL IGHNRTVEEI REYIGAESLQ YLTLEGLMKS VNIGIKPQAE 

       490        500        510        520 
TNSTPCFSTS SPTTTKIKIN ENNQKHCVAC FTGDYPCSLD F 

« Hide

References

[1]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000012 Genomic DNA. Translation: EAL70053.1.
RefSeqXP_643947.1. XM_638855.1.

3D structure databases

ProteinModelPortalQ86A85.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0230083.

Protein family/group databases

MEROPSC44.001.

Proteomic databases

PRIDEQ86A85.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0230083; DDB0230083; DDB_G0274321.
GeneID8619375.
KEGGddi:DDB_G0274321.

Organism-specific databases

dictyBaseDDB_G0274321. purF.

Phylogenomic databases

eggNOGCOG0034.
OMAACYGCFT.
PhylomeDBQ86A85.

Enzyme and pathway databases

UniPathwayUPA00074; UER00124.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ86A85.

Entry information

Entry namePUR1_DICDI
AccessionPrimary (citable) accession number: Q86A85
Secondary accession number(s): Q555W3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase