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Q86A67 (ALF_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fructose-bisphosphate aldolase

Short name=ALDO
EC=4.1.2.13
Gene names
Name:fba
ORF Names:DDB_G0274375
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Sequence similarities

Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
   Biological processGlycolysis
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processfructose metabolic process

Inferred from sequence or structural similarity. Source: dictyBase

glycolysis

Inferred from sequence or structural similarity. Source: dictyBase

   Cellular_componentcytosol

Inferred by curator. Source: dictyBase

phagocytic vesicle

Inferred from direct assay PubMed 19482547. Source: dictyBase

   Molecular_functionfructose-bisphosphate aldolase activity

Inferred from sequence or structural similarity. Source: dictyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357Fructose-bisphosphate aldolase
PRO_0000327835

Sites

Active site1831Proton acceptor By similarity
Active site2251Schiff-base intermediate with dihydroxyacetone-P By similarity
Binding site491Substrate By similarity
Binding site1401Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q86A67 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: C6906B8DBB8CCF19

FASTA35738,903
        10         20         30         40         50         60 
MNSKFADELI ATAKAIVAPG KGILAADEST NTIGARFKKI NLENNEENRR AYRELLIGTG 

        70         80         90        100        110        120 
NGVNEFIGGI ILYEETLYQK MADGRLFTDV LREQKIHIGI KVDKGVVPIP GTDGETSTQG 

       130        140        150        160        170        180 
LDGLAERCKK YYEAGARFAK WRAVLKIDLA KNCPSQLSIT ENAHTLARYA AICQENGLVP 

       190        200        210        220        230        240 
IVEPEILMDG NHTVEQSAEV TEKVLAAVFK ALNDHHILLE GALLKPNMVV NGTDCPIKAT 

       250        260        270        280        290        300 
SEQIGKFTVR TLQRTVPPAL TGVVFLSGGQ TEIEATANLN AMNVLPNRPW ALSFSYGRAL 

       310        320        330        340        350 
QASVISTWKG ESANVEAARK VYLHRAKCNS LAQLGKYTGE ESTGSASESL YVKDYKY 

« Hide

References

[1]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000012 Genomic DNA. Translation: EAL70080.1.

3D structure databases

ProteinModelPortalQ86A67.
SMRQ86A67. Positions 6-355.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0231387.

Proteomic databases

PRIDEQ86A67.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231387; DDB0231387; DDB_G0274375.
KEGGddi:DDB_G0274375.

Organism-specific databases

dictyBaseDDB_G0274375. fba.

Phylogenomic databases

eggNOGCOG3588.
KOK01623.
OMARGQQDNA.
PhylomeDBQ86A67.
ProtClustDBPTZ00019.

Enzyme and pathway databases

UniPathwayUPA00109; UER00183.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR000741. Aldolase_I.
IPR013785. Aldolase_TIM.
[Graphical view]
PANTHERPTHR11627. PTHR11627. 1 hit.
PfamPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALF_DICDI
AccessionPrimary (citable) accession number: Q86A67
Secondary accession number(s): Q556D6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase