Q86A67 (ALF_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase
Short name=ALDO
EC=4.1.2.13

Gene names

Name:	fba
ORF Names:	DDB_G0274375

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fructose metabolic process Inferred from sequence or structural similarity. Source: dictyBase glycolysis Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	cytosol Inferred by curator. Source: dictyBase extracellular space Inferred from direct assay. Source: dictyBase phagocytic vesicle Inferred from direct assay. Source: dictyBase
Molecular function	fructose-bisphosphate aldolase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Fructose-bisphosphate aldolase		PRO_0000327835
Sites
	Active site	183	1	Proton acceptor By similarity
	Active site	225	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	49	1	Substrate By similarity
	Binding site	140	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q86A67 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C6906B8DBB8CCF19	FASTA	357	38,903
	10 20 30 40 50 60 MNSKFADELI ATAKAIVAPG KGILAADEST NTIGARFKKI NLENNEENRR AYRELLIGTG 70 80 90 100 110 120 NGVNEFIGGI ILYEETLYQK MADGRLFTDV LREQKIHIGI KVDKGVVPIP GTDGETSTQG 130 140 150 160 170 180 LDGLAERCKK YYEAGARFAK WRAVLKIDLA KNCPSQLSIT ENAHTLARYA AICQENGLVP 190 200 210 220 230 240 IVEPEILMDG NHTVEQSAEV TEKVLAAVFK ALNDHHILLE GALLKPNMVV NGTDCPIKAT 250 260 270 280 290 300 SEQIGKFTVR TLQRTVPPAL TGVVFLSGGQ TEIEATANLN AMNVLPNRPW ALSFSYGRAL 310 320 330 340 350 QASVISTWKG ESANVEAARK VYLHRAKCNS LAQLGKYTGE ESTGSASESL YVKDYKY

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000012 Genomic DNA. Translation: EAL70080.1.
RefSeq	XP_643874.1. XM_638782.1.
3D structure databases
HSSP	HSSP built from PDB template 1XDL based on UniProtKB P05062.
ProteinModelPortal	Q86A67.
SMR	Q86A67. Positions 6-355.
ModBase	Search...
Protein-protein interaction databases
STRING	Q86A67.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231387; DDB0231387; DDB_G0274375.
GeneID	8619300.
GenomeReviews	Gene locus fba in contig CM000151_GR.
KEGG	ddi:DDB_G0274375.
Organism-specific databases
dictyBase	DDB_G0274375. fba.
Phylogenomic databases
eggNOG	KOG1557.
GeneTree	EPrGT00050000006055.
HOGENOM	HBG559178.
OMA	ACPIKYT.
PhylomeDB	Q86A67.
ProtClustDB	PTZ00019.
Family and domain databases
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01623.
PANTHER	PTHR11627. Aldolase_I. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF_DICDI

Accession

Primary (citable) accession number: Q86A67
Secondary accession number(s): Q556D6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)