Q86A17 (DHPR_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydropteridine reductase
EC=1.5.1.34

Alternative name(s):
Quinoid dihydropteridine reductase

Gene names

Name:	qdpr
ORF Names:	DDB_G0272684

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The product of this enzyme, tetrahydrobiopterin (BH-4), is an essential cofactor for phenylalanine, tyrosine, and tryptophan hydroxylases By similarity.
Catalytic activity	A 5,6,7,8-tetrahydropteridine + NAD(P)+ = a 6,7-dihydropteridine + NAD(P)H.
Subunit structure	Homodimer. Ref.3
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Tetrahydrobiopterin biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	dihydrobiopterin metabolic process Inferred from sequence or structural similarity. Source: dictyBase tetrahydrobiopterin biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	phagocytic vesicle Inferred from direct assay. Source: dictyBase
Molecular function	6,7-dihydropteridine reductase activity Inferred from sequence or structural similarity. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro protein homodimerization activity Inferred from direct assay Ref.3. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 231

231

Dihydropteridine reductase

PRO_0000327797

Regions

Nucleotide binding

6 – 30

25

NADP By similarity

Sites

Active site

138

1

Proton acceptor By similarity

Secondary structure

1

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231

Helix Strand Turn

Details...

Beta strand

4 – 8

5

Turn

9 – 11

3

Helix

13 – 24

12

Beta strand

28 – 35

8

Beta strand

40 – 45

6

Helix

51 – 62

12

Turn

63 – 65

3

Beta strand

68 – 73

6

Helix

88 – 113

26

Beta strand

114 – 123

10

Helix

126 – 129

4

Helix

136 – 152

17

Beta strand

157 – 159

3

Beta strand

164 – 171

8

Helix

176 – 181

6

Helix

187 – 189

3

Helix

193 – 205

13

Helix

207 – 209

3

Beta strand

217 – 222

6

Beta strand

225 – 230

6

Sequences

	Sequence		Length	Mass (Da)
	Q86A17 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 0D5E03DFF4F6AA14	FASTA	231	24,651
	10 20 30 40 50 60 MSKNILVLGG SGALGAEVVK FFKSKSWNTI SIDFRENPNA DHSFTIKDSG EEEIKSVIEK 70 80 90 100 110 120 INSKSIKVDT FVCAAGGWSG GNASSDEFLK SVKGMIDMNL YSAFASAHIG AKLLNQGGLF 130 140 150 160 170 180 VLTGASAALN RTSGMIAYGA TKAATHHIIK DLASENGGLP AGSTSLGILP VTLDTPTNRK 190 200 210 220 230 YMSDANFDDW TPLSEVAEKL FEWSTNSDSR PTNGSLVKFE TKSKVTTWTN L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Crystallization and preliminary characterization of dihydropteridine reductase from Dictyostelium discoideum." Chen C., Seo K.H., Kim H.L., Zhuang N., Park Y.S., Lee K.H. Acta Crystallogr. F 64:1013-1015(2008) [PubMed: 18997329] [Abstract] Cited for: SUBUNIT, CRYSTALLIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AAFI02000008 Genomic DNA. Translation: EAL70979.1.

RefSeq

XP_644903.1. XM_639811.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ORF	X-ray	2.16	A/B/C/D	1-231	[»]

ProteinModelPortal

Q86A17.

ModBase

Search...

Protein-protein interaction databases

STRING

Q86A17.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblProtists

DDB0237752; DDB0237752; DDB_G0272684.

GeneID

8618582.

GenomeReviews

Gene locus qdpr in contig CM000151_GR.

KEGG

ddi:DDB_G0272684.

Organism-specific databases

dictyBase

DDB_G0272684. qdpr.

Phylogenomic databases

eggNOG

KOG4022.

GeneTree

EPrGT00050000005684.

HOGENOM

HBG380758.

OMA

HETSRES.

PhylomeDB

Q86A17.

ProtClustDB

CLSZ2431259.

Family and domain databases

InterPro

IPR002198. DH_sc/Rdtase_SDR.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

KO

K00357.

Pfam

PF00106. adh_short. 1 hit.
[Graphical view]

PROSITE

PS00061. ADH_SHORT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DHPR_DICDI

Accession

Primary (citable) accession number: Q86A17
Secondary accession number(s): Q559B3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)