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Q869Z4

- PCKGM_DICDI

UniProt

Q869Z4 - PCKGM_DICDI

Protein

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Gene

pck2

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

    Cofactori

    Manganese.By similarity
    Binds 1 manganese ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181SubstrateBy similarity
    Binding sitei259 – 2591Substrate; via amide nitrogenBy similarity
    Metal bindingi266 – 2661ManganeseBy similarity
    Binding sitei266 – 2661SubstrateBy similarity
    Metal bindingi286 – 2861Manganese; via tele nitrogenBy similarity
    Binding sitei308 – 3081SubstrateBy similarity
    Active sitei310 – 3101By similarity
    Metal bindingi333 – 3331ManganeseBy similarity
    Binding sitei426 – 4261GTPBy similarity
    Binding sitei457 – 4571GTPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi309 – 3146GTPBy similarity
    Nucleotide bindingi555 – 5584GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_212905. Abacavir metabolism.
    REACT_224397. Transcriptional regulation of white adipocyte differentiation.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (EC:4.1.1.32)
    Short name:
    PEPCK-M
    Gene namesi
    Name:pck2
    ORF Names:DDB_G0271904
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0271904.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5151MitochondrionBy similarityAdd
    BLAST
    Chaini52 – 653602Phosphoenolpyruvate carboxykinase [GTP], mitochondrialPRO_0000328603Add
    BLAST

    Proteomic databases

    PRIDEiQ869Z4.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi44689.DDB_0230145.

    Structurei

    3D structure databases

    ProteinModelPortaliQ869Z4.
    SMRiQ869Z4. Positions 51-651.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni424 – 4263Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1274.
    KOiK01596.
    OMAiWDNPAGV.
    PhylomeDBiQ869Z4.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q869Z4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKNFIQKGK LINSISSSSS FQSSTFLNNN GFNSGAWVGS RFFSLEQLKK    50
    KTKNQKLINW VEEQAKLCKP DNIYICDGSE EEFKKFTDDM VKSGTLIKLN 100
    SKRPNSFLAR SDPSDVARVE SRTYICAKTK EDAGPTNNWM DPNQMKQTLK 150
    PLFNGSMKGR TMYVMPFSMG PLGSDISHIG VQVTDSPFVV CNMKIMTRMG 200
    DKVLNQIKEN DDFVPCLHSV GAPLAKGQKD SHWPCNPNKY IVHYPEERSI 250
    QSFGSGYGGN ALLGKKCFSL RIASSMAKEG GWLAEHMLIL SLTNDKGEKK 300
    YIAAAFPSAC GKTNLAMANS VLPGYKVQVC GDDIAWMTMR NGQLYAINPE 350
    FGFFGVAPGT SASSNPNALA AISKNTLFTN VALTPDNDVW WEGLSSPPPT 400
    AIDWLGNEWH PNGEGNNTFA AHPNSRFTAP LSQCPSIDPE WNNPTGVPIS 450
    AVIFGGRRSS TVPLVYQALN WKHGVFMGAS TASELTAAAE GTVGTLRHDP 500
    FAMLPFCGYN MGDYFAHWLS MEQKAGSNAD KLPKIFYVNW FRKNKTSGKF 550
    LWPGFGENVR VLKWIFDRCN STLDTTDGKA TQTPIGFVPS NNSIDLNGLE 600
    INKSSLKELF SLNKSEWLHD LKSMRQFCQQ FGDRLPNEIK NQMDQLENRL 650
    NKD 653
    Length:653
    Mass (Da):72,462
    Last modified:June 1, 2003 - v1
    Checksum:iBDE051CA1E245333
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000007 Genomic DNA. Translation: EAL71476.1.
    RefSeqiXP_645396.1. XM_640304.1.

    Genome annotation databases

    EnsemblProtistsiDDB0230145; DDB0230145; DDB_G0271904.
    GeneIDi8618224.
    KEGGiddi:DDB_G0271904.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000007 Genomic DNA. Translation: EAL71476.1 .
    RefSeqi XP_645396.1. XM_640304.1.

    3D structure databases

    ProteinModelPortali Q869Z4.
    SMRi Q869Z4. Positions 51-651.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0230145.

    Proteomic databases

    PRIDEi Q869Z4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0230145 ; DDB0230145 ; DDB_G0271904 .
    GeneIDi 8618224.
    KEGGi ddi:DDB_G0271904.

    Organism-specific databases

    dictyBasei DDB_G0271904.

    Phylogenomic databases

    eggNOGi COG1274.
    KOi K01596.
    OMAi WDNPAGV.
    PhylomeDBi Q869Z4.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    Reactomei REACT_212905. Abacavir metabolism.
    REACT_224397. Transcriptional regulation of white adipocyte differentiation.

    Miscellaneous databases

    PROi Q869Z4.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiPCKGM_DICDI
    AccessioniPrimary (citable) accession number: Q869Z4
    Secondary accession number(s): Q55AF8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3