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Q869Z4

- PCKGM_DICDI

UniProt

Q869Z4 - PCKGM_DICDI

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Protein

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Gene
pck2, DDB_G0271904
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Manganese By similarity.UniRule annotation
Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Substrate By similarity
Binding sitei259 – 2591Substrate; via amide nitrogen By similarity
Metal bindingi266 – 2661Manganese By similarity
Binding sitei266 – 2661Substrate By similarity
Metal bindingi286 – 2861Manganese; via tele nitrogen By similarity
Binding sitei308 – 3081Substrate By similarity
Active sitei310 – 3101 By similarity
Metal bindingi333 – 3331Manganese By similarity
Binding sitei426 – 4261GTP By similarity
Binding sitei457 – 4571GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi309 – 3146GTP By similarity
Nucleotide bindingi555 – 5584GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_212905. Abacavir metabolism.
REACT_224397. Transcriptional regulation of white adipocyte differentiation.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (EC:4.1.1.32)
Short name:
PEPCK-M
Gene namesi
Name:pck2
ORF Names:DDB_G0271904
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0271904.

Subcellular locationi

Mitochondrion Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5151Mitochondrion By similarityAdd
BLAST
Chaini52 – 653602Phosphoenolpyruvate carboxykinase [GTP], mitochondrialUniRule annotationPRO_0000328603Add
BLAST

Proteomic databases

PRIDEiQ869Z4.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi44689.DDB_0230145.

Structurei

3D structure databases

ProteinModelPortaliQ869Z4.
SMRiQ869Z4. Positions 51-651.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni424 – 4263Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1274.
KOiK01596.
OMAiWDNPAGV.
PhylomeDBiQ869Z4.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q869Z4-1 [UniParc]FASTAAdd to Basket

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MLKNFIQKGK LINSISSSSS FQSSTFLNNN GFNSGAWVGS RFFSLEQLKK    50
KTKNQKLINW VEEQAKLCKP DNIYICDGSE EEFKKFTDDM VKSGTLIKLN 100
SKRPNSFLAR SDPSDVARVE SRTYICAKTK EDAGPTNNWM DPNQMKQTLK 150
PLFNGSMKGR TMYVMPFSMG PLGSDISHIG VQVTDSPFVV CNMKIMTRMG 200
DKVLNQIKEN DDFVPCLHSV GAPLAKGQKD SHWPCNPNKY IVHYPEERSI 250
QSFGSGYGGN ALLGKKCFSL RIASSMAKEG GWLAEHMLIL SLTNDKGEKK 300
YIAAAFPSAC GKTNLAMANS VLPGYKVQVC GDDIAWMTMR NGQLYAINPE 350
FGFFGVAPGT SASSNPNALA AISKNTLFTN VALTPDNDVW WEGLSSPPPT 400
AIDWLGNEWH PNGEGNNTFA AHPNSRFTAP LSQCPSIDPE WNNPTGVPIS 450
AVIFGGRRSS TVPLVYQALN WKHGVFMGAS TASELTAAAE GTVGTLRHDP 500
FAMLPFCGYN MGDYFAHWLS MEQKAGSNAD KLPKIFYVNW FRKNKTSGKF 550
LWPGFGENVR VLKWIFDRCN STLDTTDGKA TQTPIGFVPS NNSIDLNGLE 600
INKSSLKELF SLNKSEWLHD LKSMRQFCQQ FGDRLPNEIK NQMDQLENRL 650
NKD 653
Length:653
Mass (Da):72,462
Last modified:June 1, 2003 - v1
Checksum:iBDE051CA1E245333
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000007 Genomic DNA. Translation: EAL71476.1.
RefSeqiXP_645396.1. XM_640304.1.

Genome annotation databases

EnsemblProtistsiDDB0230145; DDB0230145; DDB_G0271904.
GeneIDi8618224.
KEGGiddi:DDB_G0271904.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000007 Genomic DNA. Translation: EAL71476.1 .
RefSeqi XP_645396.1. XM_640304.1.

3D structure databases

ProteinModelPortali Q869Z4.
SMRi Q869Z4. Positions 51-651.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0230145.

Proteomic databases

PRIDEi Q869Z4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0230145 ; DDB0230145 ; DDB_G0271904 .
GeneIDi 8618224.
KEGGi ddi:DDB_G0271904.

Organism-specific databases

dictyBasei DDB_G0271904.

Phylogenomic databases

eggNOGi COG1274.
KOi K01596.
OMAi WDNPAGV.
PhylomeDBi Q869Z4.

Enzyme and pathway databases

UniPathwayi UPA00138 .
Reactomei REACT_212905. Abacavir metabolism.
REACT_224397. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

PROi Q869Z4.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPCKGM_DICDI
AccessioniPrimary (citable) accession number: Q869Z4
Secondary accession number(s): Q55AF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: September 3, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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