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Protein

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Gene

pck2

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118SubstrateBy similarity1
Metal bindingi266ManganeseBy similarity1
Metal bindingi286Manganese; via tele nitrogenBy similarity1
Binding sitei308SubstrateBy similarity1
Active sitei310By similarity1
Metal bindingi333ManganeseBy similarity1
Binding sitei426GTPBy similarity1
Binding sitei457GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi309 – 314GTPBy similarity6
Nucleotide bindingi555 – 558GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-70263 Gluconeogenesis
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (EC:4.1.1.32)
Short name:
PEPCK-M
Gene namesi
Name:pck2
ORF Names:DDB_G0271904
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0271904

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 51MitochondrionBy similarityAdd BLAST51
ChainiPRO_000032860352 – 653Phosphoenolpyruvate carboxykinase [GTP], mitochondrialAdd BLAST602

Proteomic databases

PaxDbiQ869Z4
PRIDEiQ869Z4

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi44689.DDB0230145

Structurei

3D structure databases

ProteinModelPortaliQ869Z4
SMRiQ869Z4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni257 – 259Substrate bindingBy similarity3
Regioni424 – 426Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3749 Eukaryota
COG1274 LUCA
InParanoidiQ869Z4
KOiK01596
OMAiCWLALTD
PhylomeDBiQ869Z4

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q869Z4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKNFIQKGK LINSISSSSS FQSSTFLNNN GFNSGAWVGS RFFSLEQLKK
60 70 80 90 100
KTKNQKLINW VEEQAKLCKP DNIYICDGSE EEFKKFTDDM VKSGTLIKLN
110 120 130 140 150
SKRPNSFLAR SDPSDVARVE SRTYICAKTK EDAGPTNNWM DPNQMKQTLK
160 170 180 190 200
PLFNGSMKGR TMYVMPFSMG PLGSDISHIG VQVTDSPFVV CNMKIMTRMG
210 220 230 240 250
DKVLNQIKEN DDFVPCLHSV GAPLAKGQKD SHWPCNPNKY IVHYPEERSI
260 270 280 290 300
QSFGSGYGGN ALLGKKCFSL RIASSMAKEG GWLAEHMLIL SLTNDKGEKK
310 320 330 340 350
YIAAAFPSAC GKTNLAMANS VLPGYKVQVC GDDIAWMTMR NGQLYAINPE
360 370 380 390 400
FGFFGVAPGT SASSNPNALA AISKNTLFTN VALTPDNDVW WEGLSSPPPT
410 420 430 440 450
AIDWLGNEWH PNGEGNNTFA AHPNSRFTAP LSQCPSIDPE WNNPTGVPIS
460 470 480 490 500
AVIFGGRRSS TVPLVYQALN WKHGVFMGAS TASELTAAAE GTVGTLRHDP
510 520 530 540 550
FAMLPFCGYN MGDYFAHWLS MEQKAGSNAD KLPKIFYVNW FRKNKTSGKF
560 570 580 590 600
LWPGFGENVR VLKWIFDRCN STLDTTDGKA TQTPIGFVPS NNSIDLNGLE
610 620 630 640 650
INKSSLKELF SLNKSEWLHD LKSMRQFCQQ FGDRLPNEIK NQMDQLENRL

NKD
Length:653
Mass (Da):72,462
Last modified:June 1, 2003 - v1
Checksum:iBDE051CA1E245333
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000007 Genomic DNA Translation: EAL71476.1
RefSeqiXP_645396.1, XM_640304.1

Genome annotation databases

EnsemblProtistsiEAL71476; EAL71476; DDB_G0271904
GeneIDi8618224
KEGGiddi:DDB_G0271904

Entry informationi

Entry nameiPCKGM_DICDI
AccessioniPrimary (citable) accession number: Q869Z4
Secondary accession number(s): Q55AF8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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