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Q869Z4

- PCKGM_DICDI

UniProt

Q869Z4 - PCKGM_DICDI

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Protein

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Gene

pck2

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Manganese.By similarity
Binds 1 manganese ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181SubstrateBy similarity
Binding sitei259 – 2591Substrate; via amide nitrogenBy similarity
Metal bindingi266 – 2661ManganeseBy similarity
Binding sitei266 – 2661SubstrateBy similarity
Metal bindingi286 – 2861Manganese; via tele nitrogenBy similarity
Binding sitei308 – 3081SubstrateBy similarity
Active sitei310 – 3101By similarity
Metal bindingi333 – 3331ManganeseBy similarity
Binding sitei426 – 4261GTPBy similarity
Binding sitei457 – 4571GTPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi309 – 3146GTPBy similarity
Nucleotide bindingi555 – 5584GTPBy similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_212905. Abacavir metabolism.
REACT_224397. Transcriptional regulation of white adipocyte differentiation.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (EC:4.1.1.32)
Short name:
PEPCK-M
Gene namesi
Name:pck2
ORF Names:DDB_G0271904
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0271904.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5151MitochondrionBy similarityAdd
BLAST
Chaini52 – 653602Phosphoenolpyruvate carboxykinase [GTP], mitochondrialPRO_0000328603Add
BLAST

Proteomic databases

PRIDEiQ869Z4.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi44689.DDB_0230145.

Structurei

3D structure databases

ProteinModelPortaliQ869Z4.
SMRiQ869Z4. Positions 51-651.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni424 – 4263Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1274.
InParanoidiQ869Z4.
KOiK01596.
OMAiWDNPAGV.
PhylomeDBiQ869Z4.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q869Z4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKNFIQKGK LINSISSSSS FQSSTFLNNN GFNSGAWVGS RFFSLEQLKK
60 70 80 90 100
KTKNQKLINW VEEQAKLCKP DNIYICDGSE EEFKKFTDDM VKSGTLIKLN
110 120 130 140 150
SKRPNSFLAR SDPSDVARVE SRTYICAKTK EDAGPTNNWM DPNQMKQTLK
160 170 180 190 200
PLFNGSMKGR TMYVMPFSMG PLGSDISHIG VQVTDSPFVV CNMKIMTRMG
210 220 230 240 250
DKVLNQIKEN DDFVPCLHSV GAPLAKGQKD SHWPCNPNKY IVHYPEERSI
260 270 280 290 300
QSFGSGYGGN ALLGKKCFSL RIASSMAKEG GWLAEHMLIL SLTNDKGEKK
310 320 330 340 350
YIAAAFPSAC GKTNLAMANS VLPGYKVQVC GDDIAWMTMR NGQLYAINPE
360 370 380 390 400
FGFFGVAPGT SASSNPNALA AISKNTLFTN VALTPDNDVW WEGLSSPPPT
410 420 430 440 450
AIDWLGNEWH PNGEGNNTFA AHPNSRFTAP LSQCPSIDPE WNNPTGVPIS
460 470 480 490 500
AVIFGGRRSS TVPLVYQALN WKHGVFMGAS TASELTAAAE GTVGTLRHDP
510 520 530 540 550
FAMLPFCGYN MGDYFAHWLS MEQKAGSNAD KLPKIFYVNW FRKNKTSGKF
560 570 580 590 600
LWPGFGENVR VLKWIFDRCN STLDTTDGKA TQTPIGFVPS NNSIDLNGLE
610 620 630 640 650
INKSSLKELF SLNKSEWLHD LKSMRQFCQQ FGDRLPNEIK NQMDQLENRL

NKD
Length:653
Mass (Da):72,462
Last modified:June 1, 2003 - v1
Checksum:iBDE051CA1E245333
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000007 Genomic DNA. Translation: EAL71476.1.
RefSeqiXP_645396.1. XM_640304.1.

Genome annotation databases

EnsemblProtistsiDDB0230145; DDB0230145; DDB_G0271904.
GeneIDi8618224.
KEGGiddi:DDB_G0271904.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000007 Genomic DNA. Translation: EAL71476.1 .
RefSeqi XP_645396.1. XM_640304.1.

3D structure databases

ProteinModelPortali Q869Z4.
SMRi Q869Z4. Positions 51-651.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0230145.

Proteomic databases

PRIDEi Q869Z4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0230145 ; DDB0230145 ; DDB_G0271904 .
GeneIDi 8618224.
KEGGi ddi:DDB_G0271904.

Organism-specific databases

dictyBasei DDB_G0271904.

Phylogenomic databases

eggNOGi COG1274.
InParanoidi Q869Z4.
KOi K01596.
OMAi WDNPAGV.
PhylomeDBi Q869Z4.

Enzyme and pathway databases

UniPathwayi UPA00138 .
Reactomei REACT_212905. Abacavir metabolism.
REACT_224397. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

PROi Q869Z4.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPCKGM_DICDI
AccessioniPrimary (citable) accession number: Q869Z4
Secondary accession number(s): Q55AF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3