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Protein

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Gene

pck2

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181SubstrateBy similarity
Binding sitei259 – 2591Substrate; via amide nitrogenBy similarity
Metal bindingi266 – 2661ManganeseBy similarity
Binding sitei266 – 2661SubstrateBy similarity
Metal bindingi286 – 2861Manganese; via tele nitrogenBy similarity
Binding sitei308 – 3081SubstrateBy similarity
Active sitei310 – 3101By similarity
Metal bindingi333 – 3331ManganeseBy similarity
Binding sitei426 – 4261GTPBy similarity
Binding sitei457 – 4571GTPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi309 – 3146GTPBy similarity
Nucleotide bindingi555 – 5584GTPBy similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (EC:4.1.1.32)
Short name:
PEPCK-M
Gene namesi
Name:pck2
ORF Names:DDB_G0271904
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0271904.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5151MitochondrionBy similarityAdd
BLAST
Chaini52 – 653602Phosphoenolpyruvate carboxykinase [GTP], mitochondrialPRO_0000328603Add
BLAST

Proteomic databases

PRIDEiQ869Z4.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi44689.DDB_0230145.

Structurei

3D structure databases

ProteinModelPortaliQ869Z4.
SMRiQ869Z4. Positions 51-651.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni424 – 4263Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1274.
InParanoidiQ869Z4.
KOiK01596.
OMAiWMSESDW.
PhylomeDBiQ869Z4.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q869Z4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKNFIQKGK LINSISSSSS FQSSTFLNNN GFNSGAWVGS RFFSLEQLKK
60 70 80 90 100
KTKNQKLINW VEEQAKLCKP DNIYICDGSE EEFKKFTDDM VKSGTLIKLN
110 120 130 140 150
SKRPNSFLAR SDPSDVARVE SRTYICAKTK EDAGPTNNWM DPNQMKQTLK
160 170 180 190 200
PLFNGSMKGR TMYVMPFSMG PLGSDISHIG VQVTDSPFVV CNMKIMTRMG
210 220 230 240 250
DKVLNQIKEN DDFVPCLHSV GAPLAKGQKD SHWPCNPNKY IVHYPEERSI
260 270 280 290 300
QSFGSGYGGN ALLGKKCFSL RIASSMAKEG GWLAEHMLIL SLTNDKGEKK
310 320 330 340 350
YIAAAFPSAC GKTNLAMANS VLPGYKVQVC GDDIAWMTMR NGQLYAINPE
360 370 380 390 400
FGFFGVAPGT SASSNPNALA AISKNTLFTN VALTPDNDVW WEGLSSPPPT
410 420 430 440 450
AIDWLGNEWH PNGEGNNTFA AHPNSRFTAP LSQCPSIDPE WNNPTGVPIS
460 470 480 490 500
AVIFGGRRSS TVPLVYQALN WKHGVFMGAS TASELTAAAE GTVGTLRHDP
510 520 530 540 550
FAMLPFCGYN MGDYFAHWLS MEQKAGSNAD KLPKIFYVNW FRKNKTSGKF
560 570 580 590 600
LWPGFGENVR VLKWIFDRCN STLDTTDGKA TQTPIGFVPS NNSIDLNGLE
610 620 630 640 650
INKSSLKELF SLNKSEWLHD LKSMRQFCQQ FGDRLPNEIK NQMDQLENRL

NKD
Length:653
Mass (Da):72,462
Last modified:June 1, 2003 - v1
Checksum:iBDE051CA1E245333
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000007 Genomic DNA. Translation: EAL71476.1.
RefSeqiXP_645396.1. XM_640304.1.

Genome annotation databases

EnsemblProtistsiDDB0230145; DDB0230145; DDB_G0271904.
GeneIDi8618224.
KEGGiddi:DDB_G0271904.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000007 Genomic DNA. Translation: EAL71476.1.
RefSeqiXP_645396.1. XM_640304.1.

3D structure databases

ProteinModelPortaliQ869Z4.
SMRiQ869Z4. Positions 51-651.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB_0230145.

Proteomic databases

PRIDEiQ869Z4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0230145; DDB0230145; DDB_G0271904.
GeneIDi8618224.
KEGGiddi:DDB_G0271904.

Organism-specific databases

dictyBaseiDDB_G0271904.

Phylogenomic databases

eggNOGiCOG1274.
InParanoidiQ869Z4.
KOiK01596.
OMAiWMSESDW.
PhylomeDBiQ869Z4.

Enzyme and pathway databases

UniPathwayiUPA00138.

Miscellaneous databases

PROiQ869Z4.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPCKGM_DICDI
AccessioniPrimary (citable) accession number: Q869Z4
Secondary accession number(s): Q55AF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: January 7, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.