Q869Z0 (Y5025_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative protein disulfide-isomerase DDB_G0275025
EC=5.3.4.1

Gene names

ORF Names:

DDB_G0275025

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glycerol ether metabolic process Inferred from electronic annotation. Source: InterPro protein folding Inferred from electronic annotation. Source: GOC
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro protein disulfide isomerase activity Inferred from electronic annotation. Source: EC protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 409	388	Putative protein disulfide-isomerase DDB_G0275025		PRO_0000388368
Regions
	Domain	28 – 140	113	Thioredoxin
	Motif	406 – 409	4	Prevents secretion from ER Potential
	Compositional bias	246 – 258	13	Poly-Asn
	Compositional bias	262 – 267	6	Poly-Thr
Amino acid modifications
	Disulfide bond	57 ↔ 60		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q869Z0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D631EC73ADC097D5	FASTA	409	46,536
	10 20 30 40 50 60 MKLINICIFI FAIICIESTF GFYTDNSNVI NLTKKNFQQQ VLNSQQNWMV EFYAPWCGHC 70 80 90 100 110 120 KSLKPEYEKV SNNLKGLVKI GAINCDEEKE LCGQYQIQGF PTLKFFSTNP KTGKKGQPED 130 140 150 160 170 180 YQGARSASEI AKFSLAKLPS NHIQKVSQDN INKFLTGTSD AKALLFTDKP KTTDLYKALS 190 200 210 220 230 240 VDFFKTLTLG EARNLNKETL EKFNIDKFPT LLVFTNDDGE TFTKFDGKLT HSTIYKFLEP 250 260 270 280 290 300 FSKKSNNDNN NNNNNNNNEE STKTTTTEKD PASEKFIEIK DEKSFEKSCS TGLCIVALFD 310 320 330 340 350 360 QSSIDDKELN EKYLELLNTV SQNFIGRMKF VWVDVSVHDK IVPQFDLSGT PNIFVINNSK 370 380 390 400 KRYTPFMGSF SDESLNSFFK SVLSGLKKAI PFTDSPKFNS QQKKQKDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	Bienvenut W.V., Ura S., Insall R.H. Submitted (JUL-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 146-171; 178-185; 237-243 AND 389-397, MASS SPECTROMETRY. Strain: AX2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000013 Genomic DNA. Translation: EAL69793.1.
RefSeq	XP_643858.1. XM_638766.1.
3D structure databases
HSSP	HSSP built from PDB template 1X5D based on UniProtKB Q15084.
ProteinModelPortal	Q869Z0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0167375; DDB0167375; DDB_G0275025.
GeneID	8619909.
KEGG	ddi:DDB_G0275025.
Organism-specific databases
dictyBase	DDB_G0275025.
Phylogenomic databases
eggNOG	COG0526.
InParanoid	Q869Z0.
KO	K09584.
OMA	INCDEEK.
Family and domain databases
Gene3D	3.40.30.10. 3 hits.
InterPro	IPR005788. Disulphide_isomerase. IPR005746. Thioredoxin. IPR012336. Thioredoxin-like_fold. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. [Graphical view]
Pfam	PF00085. Thioredoxin. 1 hit. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
SUPFAM	SSF52833. Thiordxn-like_fd. 3 hits.
TIGRFAMs	TIGR01126. pdi_dom. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y5025_DICDI

Accession

Primary (citable) accession number: Q869Z0
Secondary accession number(s): Q553V3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	June 1, 2003
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)