Reviewed, UniProtKB/Swiss-Prot Q869E1 (DNLI1_DICDI)

Names and origin

Protein names

Recommended name:
    DNA ligase 1
    EC=6.5.1.1
Alternative name(s):
    DNA ligase I
    Polydeoxyribonucleotide synthase [ATP] 1

Gene names

Name:	lig1
ORF Names:	DDB_G0274493

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1192 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1192	1192	DNA ligase 1		PRO_0000351214
Regions
	Region	724 – 733	10	Interaction with target DNA By similarity
	Region	918 – 920	3	Interaction with target DNA By similarity
	Compositional bias	11 – 468	458	Lys-rich
	Compositional bias	13 – 512	500	Glu-rich
	Compositional bias	171 – 201	31	Asp-rich
	Compositional bias	395 – 425	31	Thr-rich
Sites
	Active site	844	1	N6-AMP-lysine intermediate By similarity
	Metal binding	897	1	Magnesium 1 By similarity
	Metal binding	996	1	Magnesium 2 By similarity
	Binding site	842	1	ATP By similarity
	Binding site	849	1	ATP By similarity
	Binding site	865	1	ATP By similarity
	Binding site	1001	1	ATP By similarity
	Binding site	1014	1	ATP By similarity
	Binding site	1020	1	ATP By similarity
	Site	580	1	Interaction with target DNA By similarity
	Site	866	1	Interaction with target DNA By similarity
	Site	1046	1	Interaction with target DNA By similarity
	Site	1071	1	Interaction with target DNA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q869E1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: B52A5842CD779766	FASTA	1,192	137,514
	10 20 30 40 50 60 MQSSMWSFLQ KKEGGEIEDI EKKISNAQEL NKLKTSPKKK REAVVKEKVE KKEKKETKPK 70 80 90 100 110 120 RKSSKKNKEE EEEEEQEEQD GEEEQEEEEE YQQQDEEIEE DINGEEEMEL DENEKEKNKK 130 140 150 160 170 180 KKQSLKTKEN KESKSSSSSK KTIENKETKK PEKQSSKQSN NLKRLKRKKM DDDEEDEEDE 190 200 210 220 230 240 NKTDDNDLDD MLDDDSDNEK DSISSKDKEY KEKVLKDKEK KEKEKKEKEL KEKESKEKEK 250 260 270 280 290 300 KEKEKKEKEE KDKKEKELKE KELKEKELKD KKEKELKEKE KELKDKEKKE KELKEKEKKE 310 320 330 340 350 360 KEEKEKEKKE KKEKELKEKE EKEKKEKELK EKELKEKELK EKELKEKELT SPKKETIDIS 370 380 390 400 410 420 DLFKRANAEA KSSVPTSTSK NSKTNKKQKV DHKPTATTKK PSPVLEAKQS TTTTTTTTTT 430 440 450 460 470 480 STATTISSKS ISSPSKKEEK EVITSKKQVE ATKVEVKKEK EKEKEKEKED DEEEEEEEED 490 500 510 520 530 540 DDEKLEDIDE EEYEEEEEED EEGISENEEE EEKKSTQIKS KFIKKVPISK KKGNAKTIQA 550 560 570 580 590 600 DLKVIGKYRP IEDAQWKKGE AVPYMVLAKT FEMMESTSSR LIIIEHLANL FRSIMLLSPK 610 620 630 640 650 660 DLVMTIYLSI NKIGPSYQSK ELGIGEHVLI KSLAESTGRS VDVIKQELTE VGDLGIIAQN 670 680 690 700 710 720 SRSTQTLMGK PTPLTIQSVF KTFQQIADLS GTGGQQKKKD LIKKLLVSCK DCETLYIIRS 730 740 750 760 770 780 LQGKLRIGLA ERSVLMALAK SVLVTPPIDG SGQQIFDIRK QMKQEEFEER YQNVVSKVTR 790 800 810 820 830 840 AYSQLPNYDL FVPHLIAVNG IDNILSTCSL KVGIPVKPML AQPTTGISQM LDRFSDMEFT 850 860 870 880 890 900 CEFKYDGERA QIHRLPDGTT HIYTRNLEDY TQKYPDIVAN VTKFVGPNVK SFILDCEAVA 910 920 930 940 950 960 FDAATKKILS FQVLSTRARK SVQLSQIKVP VCVFAFDLLY LNGQSLIDEP LIKRREHLVE 970 980 990 1000 1010 1020 NFIASDGVFA FAKYSNISDV NDIQSYLEEA VEGNCEGLMV KTLKEKSIYE PSRRSYNWLK 1030 1040 1050 1060 1070 1080 IKKDYMQGMT DSLDLVPIGA WYGKGKRTGT YGAYLLACYD ENNEEFQTLC KIGTGFSDEQ 1090 1100 1110 1120 1130 1140 LTTFTSQLKP HLINQPRNQF RFSDSIKPDV WFSPSCVWEV LAADLSISPV HTAASGILDP 1150 1160 1170 1180 1190 NKGIALRFPR FIRIRPDKSP EDATSSDQVV DMYQNQKINS QSSKINEKDE DY

References

[1]

"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000012 Genomic DNA. Translation: EAL70139.1.
RefSeq	XP_644124.1.
3D structure databases
HSSP	HSSP built from PDB template 1X9N based on UniProtKB P18858.
SMR	Q869E1. Positions 547-1177.
ModBase	Search...
Protein-protein interaction databases
STRING	Q869E1.
Genome annotation databases
GeneID	8619554.
KEGG	ddi:DDB_0232265.
Organism-specific databases
dictyBase	DDB_G0274493. lig1.
Phylogenomic databases
eggNOG	KOG0967.
HOGENOM	HBG570821.
OMA	IEEDING.
PhylomeDB	Q869E1.
Family and domain databases
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI1_DICDI

Accession

Primary (citable) accession number: Q869E1
Secondary accession number(s): Q556E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)