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Protein

Acetylcholinesterase

Gene

Ace

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Rapidly hydrolyzes choline released into the synapse.Curated

Catalytic activityi

Acetylcholine + H2O = choline + acetate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei360Acyl-ester intermediatePROSITE-ProRule annotation1
Active sitei486Charge relay systemBy similarity1
Active sitei600Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Serine esterase
Biological processNeurotransmitter degradation

Protein family/group databases

ESTHERianoga-ACHE1. AChE.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylcholinesterase (EC:3.1.1.7)
Short name:
AChE
Gene namesi
Name:Ace
Synonyms:ACE1, ACHE1
ORF Names:AGAP001356
OrganismiAnopheles gambiae (African malaria mosquito)Imported
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
Proteomesi
  • UP000007062 Componentsi: Chromosome 2R, Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell junction, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2046266.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 38Sequence analysisAdd BLAST38
ChainiPRO_000000859939 – 737AcetylcholinesteraseAdd BLAST699

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi220N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi228 ↔ 255By similarity
Disulfide bondi414 ↔ 427By similarity
Disulfide bondi562 ↔ 683By similarity
Glycosylationi670N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ869C3.

Interactioni

Protein-protein interaction databases

STRINGi7165.AGAP001356-PA.

Chemistry databases

BindingDBiQ869C3.

Structurei

Secondary structure

1737
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi173 – 176Combined sources4
Beta strandi178 – 181Combined sources4
Beta strandi187 – 195Combined sources9
Helixi202 – 204Combined sources3
Beta strandi217 – 220Combined sources4
Beta strandi227 – 229Combined sources3
Helixi240 – 243Combined sources4
Beta strandi251 – 253Combined sources3
Beta strandi257 – 265Combined sources9
Beta strandi268 – 276Combined sources9
Turni280 – 282Combined sources3
Helixi289 – 291Combined sources3
Helixi294 – 300Combined sources7
Beta strandi303 – 307Combined sources5
Helixi313 – 316Combined sources4
Beta strandi324 – 326Combined sources3
Helixi328 – 343Combined sources16
Helixi344 – 347Combined sources4
Beta strandi349 – 359Combined sources11
Helixi361 – 371Combined sources11
Turni373 – 377Combined sources5
Beta strandi380 – 386Combined sources7
Turni392 – 394Combined sources3
Helixi398 – 412Combined sources15
Helixi421 – 428Combined sources8
Helixi433 – 437Combined sources5
Beta strandi457 – 459Combined sources3
Helixi464 – 469Combined sources6
Beta strandi478 – 483Combined sources6
Helixi488 – 494Combined sources7
Beta strandi497 – 499Combined sources3
Helixi509 – 519Combined sources11
Helixi526 – 534Combined sources9
Helixi544 – 559Combined sources16
Helixi561 – 573Combined sources13
Beta strandi578 – 583Combined sources6
Helixi594 – 596Combined sources3
Helixi604 – 607Combined sources4
Helixi614 – 616Combined sources3
Helixi620 – 639Combined sources20
Beta strandi649 – 651Combined sources3
Turni659 – 661Combined sources3
Beta strandi663 – 667Combined sources5
Beta strandi674 – 679Combined sources6
Helixi680 – 687Combined sources8
Helixi689 – 697Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AZGmodel-A115-737[»]
5X61X-ray3.40A/B162-714[»]
ProteinModelPortaliQ869C3.
SMRiQ869C3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4389. Eukaryota.
COG2272. LUCA.
HOGENOMiHOG000091866.
InParanoidiQ869C3.
KOiK01049.
OMAiFDHYSKQ.
OrthoDBiEOG091G05SE.
PhylomeDBiQ869C3.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiView protein in InterPro
IPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
PfamiView protein in Pfam
PF00135. COesterase. 1 hit.
PRINTSiPR00878. CHOLNESTRASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiView protein in PROSITE
PS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q869C3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIRGLLMGR LRLGRRMVPL GLLGVTALLL ILPPFALVQG RHHELNNGAA
60 70 80 90 100
IGSHQLSAAA GVGLASQSAQ SGSLASGVMS SVPAAGASSS SSSSLLSSSA
110 120 130 140 150
EDDVARITLS KDADAFFTPY IGHGESVRII DAELGTLEHV HSGATPRRRG
160 170 180 190 200
LTRRESNSDA NDNDPLVVNT DKGRIRGITV DAPSGKKVDV WLGIPYAQPP
210 220 230 240 250
VGPLRFRHPR PAEKWTGVLN TTTPPNSCVQ IVDTVFGDFP GATMWNPNTP
260 270 280 290 300
LSEDCLYINV VAPRPRPKNA AVMLWIFGGG FYSGTATLDV YDHRALASEE
310 320 330 340 350
NVIVVSLQYR VASLGFLFLG TPEAPGNAGL FDQNLALRWV RDNIHRFGGD
360 370 380 390 400
PSRVTLFGES AGAVSVSLHL LSALSRDLFQ RAILQSGSPT APWALVSREE
410 420 430 440 450
ATLRALRLAE AVGCPHEPSK LSDAVECLRG KDPHVLVNNE WGTLGICEFP
460 470 480 490 500
FVPVVDGAFL DETPQRSLAS GRFKKTEILT GSNTEEGYYF IIYYLTELLR
510 520 530 540 550
KEEGVTVTRE EFLQAVRELN PYVNGAARQA IVFEYTDWTE PDNPNSNRDA
560 570 580 590 600
LDKMVGDYHF TCNVNEFAQR YAEEGNNVYM YLYTHRSKGN PWPRWTGVMH
610 620 630 640 650
GDEINYVFGE PLNPTLGYTE DEKDFSRKIM RYWSNFAKTG NPNPNTASSE
660 670 680 690 700
FPEWPKHTAH GRHYLELGLN TSFVGRGPRL RQCAFWKKYL PQLVAATSNL
710 720 730
PGPAPPSEPC ESSAFFYRPD LIVLLVSLLT ATVRFIQ
Length:737
Mass (Da):80,902
Last modified:April 4, 2006 - v3
Checksum:iFDE9CCAE34DF2421
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35F → S in CAD56157 (PubMed:12736674).Curated1
Sequence conflicti65A → S in CAD56157 (PubMed:12736674).Curated1

Polymorphismi

Strains Kisumu and Kisumu2 are susceptible to insecticides while strain YAO is resistant. Insensitivity to insecticides results from a loss of sensitivity of acetylcholinesterase to organophosphates and carbamates and is due to a variant at position 280.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti127V → A in strain: Kisumu2 and YAO. 1
Natural varianti280G → S in strain: YAO; confers resistance to insecticides. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ488492 Genomic DNA. Translation: CAD32684.2.
AJ515149, AJ515148 Genomic DNA. Translation: CAD56156.1.
AJ515150, AJ488492 Genomic DNA. Translation: CAD56157.2.
AAAB01008987 Genomic DNA. Translation: EAA01151.3.
BN000066 Genomic DNA. Translation: CAD29865.2.
RefSeqiXP_321792.2. XM_321792.4.

Genome annotation databases

EnsemblMetazoaiAGAP001356-RA; AGAP001356-PA; AGAP001356.
GeneIDi1281827.
KEGGiaga:AgaP_AGAP001356.
VectorBaseiAGAP001356-RA; AGAP001356-PA; AGAP001356.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiACES_ANOGA
AccessioniPrimary (citable) accession number: Q869C3
Secondary accession number(s): Q7PUR2
, Q7RTM0, Q86GC7, Q8ISM4, Q8ISM7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: April 4, 2006
Last modified: June 7, 2017
This is version 116 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families