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Protein

Acetylcholinesterase

Gene

Ace

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Rapidly hydrolyzes choline released into the synapse.Curated

Catalytic activityi

Acetylcholine + H2O = choline + acetate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei360Acyl-ester intermediatePROSITE-ProRule annotation1
Active sitei486Charge relay systemBy similarity1
Active sitei600Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Neurotransmitter degradation

Protein family/group databases

ESTHERianoga-ACHE1. AChE.
MEROPSiS09.980.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylcholinesterase (EC:3.1.1.7)
Short name:
AChE
Gene namesi
Name:Ace
Synonyms:ACE1, ACHE1
ORF Names:AGAP001356
OrganismiAnopheles gambiae (African malaria mosquito)Imported
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
Proteomesi
  • UP000007062 Componentsi: Chromosome 2R, Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2046266.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 38Sequence analysisAdd BLAST38
ChainiPRO_000000859939 – 737AcetylcholinesteraseAdd BLAST699

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi220N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi228 ↔ 255By similarity
Disulfide bondi414 ↔ 427By similarity
Disulfide bondi562 ↔ 683By similarity
Glycosylationi670N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ869C3.

Interactioni

Protein-protein interaction databases

STRINGi7165.AGAP001356-PA.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AZGmodel-A115-737[»]
ProteinModelPortaliQ869C3.
SMRiQ869C3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4389. Eukaryota.
COG2272. LUCA.
HOGENOMiHOG000091866.
InParanoidiQ869C3.
KOiK01049.
OMAiRNQMCAF.
OrthoDBiEOG091G05SE.
PhylomeDBiQ869C3.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q869C3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIRGLLMGR LRLGRRMVPL GLLGVTALLL ILPPFALVQG RHHELNNGAA
60 70 80 90 100
IGSHQLSAAA GVGLASQSAQ SGSLASGVMS SVPAAGASSS SSSSLLSSSA
110 120 130 140 150
EDDVARITLS KDADAFFTPY IGHGESVRII DAELGTLEHV HSGATPRRRG
160 170 180 190 200
LTRRESNSDA NDNDPLVVNT DKGRIRGITV DAPSGKKVDV WLGIPYAQPP
210 220 230 240 250
VGPLRFRHPR PAEKWTGVLN TTTPPNSCVQ IVDTVFGDFP GATMWNPNTP
260 270 280 290 300
LSEDCLYINV VAPRPRPKNA AVMLWIFGGG FYSGTATLDV YDHRALASEE
310 320 330 340 350
NVIVVSLQYR VASLGFLFLG TPEAPGNAGL FDQNLALRWV RDNIHRFGGD
360 370 380 390 400
PSRVTLFGES AGAVSVSLHL LSALSRDLFQ RAILQSGSPT APWALVSREE
410 420 430 440 450
ATLRALRLAE AVGCPHEPSK LSDAVECLRG KDPHVLVNNE WGTLGICEFP
460 470 480 490 500
FVPVVDGAFL DETPQRSLAS GRFKKTEILT GSNTEEGYYF IIYYLTELLR
510 520 530 540 550
KEEGVTVTRE EFLQAVRELN PYVNGAARQA IVFEYTDWTE PDNPNSNRDA
560 570 580 590 600
LDKMVGDYHF TCNVNEFAQR YAEEGNNVYM YLYTHRSKGN PWPRWTGVMH
610 620 630 640 650
GDEINYVFGE PLNPTLGYTE DEKDFSRKIM RYWSNFAKTG NPNPNTASSE
660 670 680 690 700
FPEWPKHTAH GRHYLELGLN TSFVGRGPRL RQCAFWKKYL PQLVAATSNL
710 720 730
PGPAPPSEPC ESSAFFYRPD LIVLLVSLLT ATVRFIQ
Length:737
Mass (Da):80,902
Last modified:April 4, 2006 - v3
Checksum:iFDE9CCAE34DF2421
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35F → S in CAD56157 (PubMed:12736674).Curated1
Sequence conflicti65A → S in CAD56157 (PubMed:12736674).Curated1

Polymorphismi

Strains Kisumu and Kisumu2 are susceptible to insecticides while strain YAO is resistant. Insensitivity to insecticides results from a loss of sensitivity of acetylcholinesterase to organophosphates and carbamates and is due to a variant at position 280.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti127V → A in strain: Kisumu2 and YAO. 1
Natural varianti280G → S in strain: YAO; confers resistance to insecticides. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ488492 Genomic DNA. Translation: CAD32684.2.
AJ515149, AJ515148 Genomic DNA. Translation: CAD56156.1.
AJ515150, AJ488492 Genomic DNA. Translation: CAD56157.2.
AAAB01008987 Genomic DNA. Translation: EAA01151.3.
BN000066 Genomic DNA. Translation: CAD29865.2.
RefSeqiXP_321792.2. XM_321792.4.

Genome annotation databases

EnsemblMetazoaiAGAP001356-RA; AGAP001356-PA; AGAP001356.
GeneIDi1281827.
KEGGiaga:AgaP_AGAP001356.
VectorBaseiAGAP001356-RA; AGAP001356-PA; AGAP001356.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ488492 Genomic DNA. Translation: CAD32684.2.
AJ515149, AJ515148 Genomic DNA. Translation: CAD56156.1.
AJ515150, AJ488492 Genomic DNA. Translation: CAD56157.2.
AAAB01008987 Genomic DNA. Translation: EAA01151.3.
BN000066 Genomic DNA. Translation: CAD29865.2.
RefSeqiXP_321792.2. XM_321792.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AZGmodel-A115-737[»]
ProteinModelPortaliQ869C3.
SMRiQ869C3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7165.AGAP001356-PA.

Chemistry databases

ChEMBLiCHEMBL2046266.

Protein family/group databases

ESTHERianoga-ACHE1. AChE.
MEROPSiS09.980.

Proteomic databases

PaxDbiQ869C3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAGAP001356-RA; AGAP001356-PA; AGAP001356.
GeneIDi1281827.
KEGGiaga:AgaP_AGAP001356.
VectorBaseiAGAP001356-RA; AGAP001356-PA; AGAP001356.

Organism-specific databases

CTDi1281827.

Phylogenomic databases

eggNOGiKOG4389. Eukaryota.
COG2272. LUCA.
HOGENOMiHOG000091866.
InParanoidiQ869C3.
KOiK01049.
OMAiRNQMCAF.
OrthoDBiEOG091G05SE.
PhylomeDBiQ869C3.

Miscellaneous databases

PROiQ869C3.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACES_ANOGA
AccessioniPrimary (citable) accession number: Q869C3
Secondary accession number(s): Q7PUR2
, Q7RTM0, Q86GC7, Q8ISM4, Q8ISM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: April 4, 2006
Last modified: November 30, 2016
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.