Q868M7 (Q868M7_CUCEC) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Hemolytic lectin CEL-III EMBL BAC75827.1

Gene names

Name:

cel3 EMBL BAC75827.1

Organism

Cucumaria echinata (Sea cucumber) EMBL BAC75827.1

Taxonomic identifier

40245 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Echinodermata › Eleutherozoa › Echinozoa › Holothuroidea › Dendrochirotacea › Dendrochirotida › Cucumariidae › Cucumaria

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 2Z48 PDB 1VCL PDB 2Z49 Lectin EMBL BAC75827.1 Magnesium PDB 2Z48 PDB 1VCL PDB 2Z49 Metal-binding PDB 2Z48 PDB 1VCL PDB 2Z49
Technical term	3D-structure PDB 2Z48 PDB 1VCL PDB 2Z49
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	131 – 134	4	N-acetyl-D-galactosamine binding PDB 2Z48
	Region	178 – 179	2	N-acetyl-D-galactosamine binding PDB 2Z48
	Region	191 – 194	4	N-acetyl-D-galactosamine binding PDB 2Z48
	Region	219 – 220	2	N-acetyl-D-galactosamine binding PDB 2Z48
Sites
	Metal binding	33	1	Calcium PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	34	1	Calcium; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	36	1	Calcium; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	42	1	Magnesium 1 PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	43	1	Magnesium 1; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	53	1	Calcium PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	82	1	Magnesium 1 PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	83	1	Magnesium 1; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	131	1	Calcium PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	134	1	Calcium; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	141	1	Magnesium 1; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	151	1	Calcium PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	178	1	Calcium PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	179	1	Calcium; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	181	1	Calcium; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	187	1	Magnesium 2 PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	188	1	Magnesium 2; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	198	1	Calcium PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	219	1	Calcium PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	220	1	Calcium; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	222	1	Calcium; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	228	1	Magnesium 2 PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	229	1	Magnesium 2; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	239	1	Calcium PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	266	1	Calcium PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	267	1	Calcium; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	269	1	Calcium; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	275	1	Magnesium 2 PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	276	1	Magnesium 2; via carbonyl oxygen PDB 2Z48 PDB 1VCL PDB 2Z49
	Metal binding	286	1	Calcium PDB 2Z48 PDB 1VCL PDB 2Z49
	Binding site	19	1	N-acetyl-D-galactosamine PDB 2Z48
	Binding site	117	1	N-acetyl-D-galactosamine; via carbonyl oxygen PDB 2Z48
	Binding site	147	1	N-acetyl-D-galactosamine PDB 2Z48
	Binding site	232	1	N-acetyl-D-galactosamine PDB 2Z48

Sequences

	Sequence		Length	Mass (Da)
	Q868M7 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 3E47C608446D40EC	FASTA	442	48,456
	10 20 30 40 50 60 MVSLVPCGFA QVLCTNPLDI GELRNYKSKQ CVDIVGNQGS GNIATHDCDG LSDQQIIMCG 70 80 90 100 110 120 DGTIRNEARN YCFTPDGSGN ANVMSSPCTL YPEIPSSQRW RLGRKKAFTD NGGIEQVATE 130 140 150 160 170 180 IINLASGKCL DVEGSDGTGD IGVYDCQNLD DQYFYIRSRG PELFYGRLRN EKSDLCLDVE 190 200 210 220 230 240 GSEGKGNVLM YSCEDNLDQW FRYYENGEIV NAKQGMCLDV EGSDGSGNVG IYRCDDLRDQ 250 260 270 280 290 300 MWSRPNAYCN GDYCSFLNKE SNKCLDVSGD QGTGDVGTWQ CDGLPDQRFK WVFDDWEVPT 310 320 330 340 350 360 ATWNMVGCDQ NGKVSQQISN TISFSSTVTA GVAVEVSSTI EKGVIFAKAS VSVKVTASLS 370 380 390 400 410 420 KAWTNSQSGT TAITYTCDNY DSDEEFTRGC MWQLAIETTE VKSGDLLVWN PQIIKCTRSN 430 440 TAPGCAPFTK CANEDCTFCT DI

References

[1]	"Primary structure of hemolytic lectin CEL-III from marine invertebrate Cucumaria echinata and its cDNA: structural similarity to the B-chain from plant lectin, ricin." Nakano M., Tabata S., Sugihara K., Kouzuma Y., Kimura M., Yamasaki N. Biochim. Biophys. Acta 1435:167-176(1999) [PubMed: 10561549] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	Kouzuma Y. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Crystal structure of the hemolytic lectin CEL-III isolated from the marine invertebrate Cucumaria echinata: implications of domain structure for its membrane pore-formation mechanism." Uchida T., Yamasaki T., Eto S., Sugawara H., Kurisu G., Nakagawa A., Kusunoki M., Hatakeyama T. J. Biol. Chem. 279:37133-37141(2004) [PubMed: 15194688] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 12-442 IN COMPLEX WITH CALCIUM AND MAGNESIUM.
[4]	"C-type lectin-like carbohydrate recognition of the hemolytic lectin CEL-III containing ricin-type -trefoil folds." Hatakeyama T., Unno H., Kouzuma Y., Uchida T., Eto S., Hidemura H., Kato N., Yonekura M., Kusunoki M. J. Biol. Chem. 282:37826-37835(2007) [PubMed: 17977832] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 12-442 IN COMPLEX WITH CALCIUM; MAGNESIUM AND N-ACETYL-D-GALACTOSAMINE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB109017 mRNA. Translation: BAC75827.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VCL	X-ray	1.70	A/B	12-442	[»]
2Z48	X-ray	1.70	A/B	12-442	[»]
2Z49	X-ray	1.95	A/B	12-442	[»]

ProteinModelPortal

Q868M7.

SMR

Q868M7. Positions 12-442.

ModBase

Search...

Protein family/group databases

CAZy

CBM13. Carbohydrate-Binding Module Family 13.

TCDB

1.C.96.1.1. haemolytic lectin, CEL-III (CEL-III) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008997. Ricin_B-rel_lectin.
IPR000772. Ricin_B_lectin.
[Graphical view]

Pfam

PF00652. Ricin_B_lectin. 2 hits.
[Graphical view]

SMART

SM00458. RICIN. 2 hits.
[Graphical view]

SUPFAM

SSF50370. RicinB_like. 2 hits.

PROSITE

PS50231. RICIN_B_LECTIN. 3 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q868M7_CUCEC

Accession

Primary (citable) accession number: Q868M7

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2003
Last sequence update:	June 1, 2003
Last modified:	July 27, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)