Reviewed, UniProtKB/Swiss-Prot Q867X3 (ACES_CULPP)

Names and origin

Protein names

Recommended name:
    Acetylcholinesterase
      Short name=AChE
    EC=3.1.1.7

Gene names

Name:

ACE-1

Organism

Culex pipiens pipiens (Northern house mosquito)

Taxonomic identifier

38569 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Culicinae › Culicini › Culex › Culex

Protein attributes

Sequence length	132 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Rapidly hydrolyzes choline released into the synapse.
Catalytic activity	Acetylcholine + H2O = choline + acetate. UniProtKB P22303
Subcellular location	Cell junction › synapse By similarity. Secreted By similarity. Cell membrane; Peripheral membrane protein By similarity.
Polymorphism	A number of strains are susceptible to insecticides while others are resistant. Insensitivity to insecticides results from a loss of sensitivity of acetylcholinesterase to organophosphates and carbamates and is due to a variant at position 97. Ref.1
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Biological process	Neurotransmitter degradation
Cellular component	Cell junction Cell membrane Membrane Secreted Synapse
Coding sequence diversity	Polymorphism
Molecular function	Hydrolase Serine esterase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetylcholinesterase activity Inferred from electronic annotation. Source: EC cholinesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›132	›132	Acetylcholinesterase		PRO_0000070280
Amino acid modifications
	Glycosylation	37	1	N-linked (GlcNAc...) Potential
	Disulfide bond	45 ↔ 72		By similarity UniProtKB P22303
Natural variations
	Natural variant	97	1	G → S in strain: Barriol, Espro, Padova and Praias. Ref.1
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	132	1

Sequences

	Sequence		Length	Mass (Da)
	Q867X3-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2F40C4B3FC2468E8	FASTA	132	14,543
	10 20 30 40 50 60 SGKKVDAWMG IPYAQPPLGP LRFRHPRPAE RWTGVLNATK PPNSCVQIVD TVFGDFPGAT 70 80 90 100 110 120 MWNPNTPLSE DCLYINVVVP RPRPKNAAVM LWIFGGGFYS GTATLDVYDH RTLASEENVI 130 VVSLQYRVAS LG

References

[1]

"Insecticide resistance in mosquito vectors." Weill M., Lutfalla G., Mogensen K., Chandre F., Berthomieu A., Berticat C., Pasteur N., Philips A., Fort P., Raymond M. Nature 423:136-137(2003) [PubMed: 12736674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Barriol, Bleuet, BrugesA, BrugesB, Espro, Heteren, Killcare, Padova and Praias.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ512688 Genomic DNA. Translation: CAD54760.1. AJ512690 Genomic DNA. Translation: CAD54762.1. AJ512695 Genomic DNA. Translation: CAD54767.1. AJ512696 Genomic DNA. Translation: CAD54768.1. AJ512699 Genomic DNA. Translation: CAD54771.1. AJ512701 Genomic DNA. Translation: CAD54773.1. AJ512702 Genomic DNA. Translation: CAD54774.1. AJ512708 Genomic DNA. Translation: CAD54780.1. AJ512709 Genomic DNA. Translation: CAD54781.1.
3D structure databases
HSSP	HSSP built from PDB template 1EA5 based on UniProtKB P04058.
ModBase	Search...
Genome annotation databases
UCSC	W09B12.1.1. c. elegans.
Enzyme and pathway databases
BRENDA	3.1.1.7. 293302.
Family and domain databases
InterPro	IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. IPR000997. Cholinesterase. [Graphical view]
PANTHER	PTHR11559. CarbesteraseB. 1 hit.
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PRINTS	PR00878. CHOLNESTRASE.
PROSITE	PS00122. CARBOXYLESTERASE_B_1. Partial match. PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACES_CULPP

Accession

Primary (citable) accession number: Q867X3
Secondary accession number(s): Q867X5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2003
Last sequence update:	June 1, 2003
Last modified:	October 13, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)