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Q867F4

- KA154_ANDAU

UniProt

Q867F4 - KA154_ANDAU

Protein

Potassium channel toxin alpha-KTx 15.4

Gene
N/A
Organism
Androctonus australis (Sahara scorpion)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Blocker of A-type voltage-gated potassium channels of cerebellar granular cells. The occlusion of the outer entry of the K+ conducting pore is partially reversible and affects both open and closed channels. It shares the same target in rat brain that BmTX3 (M.martensii), and AmmTX3 (A.mauretanicus).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei49 – 491Basic residue of the functional dyadBy similarity
    Sitei58 – 581Aromatic residue of the functional dyadBy similarity

    GO - Molecular functioni

    1. ion channel inhibitor activity Source: InterPro

    GO - Biological processi

    1. defense response Source: InterPro
    2. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium channel toxin alpha-KTx 15.4
    Short name:
    AaTX1
    Short name:
    Toxin Aa1
    OrganismiAndroctonus australis (Sahara scorpion)
    Taxonomic identifieri6858 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeAndroctonus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 22221 PublicationAdd
    BLAST
    Chaini23 – 5937Potassium channel toxin alpha-KTx 15.4PRO_0000035310Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei23 – 231Pyrrolidone carboxylic acid1 Publication
    Disulfide bondi30 ↔ 50By similarity
    Disulfide bondi35 ↔ 55By similarity
    Disulfide bondi39 ↔ 57By similarity

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    3D structure databases

    ProteinModelPortaliQ867F4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.30.30.10. 1 hit.
    InterProiIPR003614. Scorpion_toxin-like.
    IPR001947. Scorpion_toxinS_K_inh.
    [Graphical view]
    PfamiPF00451. Toxin_2. 1 hit.
    [Graphical view]
    ProDomiPD003586. Scorpion_toxinS. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF57095. SSF57095. 1 hit.
    PROSITEiPS01138. SCORP_SHORT_TOXIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q867F4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFSSIILLT LLICSMSIFG NCQIETNKKC QGGSCASVCR RVIGVAAGKC   50
    INGRCVCYP 59
    Length:59
    Mass (Da):6,291
    Last modified:June 1, 2003 - v1
    Checksum:iE429B1B607A2F64E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241I → N AA sequence (PubMed:11018665)Curated

    Mass spectrometryi

    Molecular mass is 3850.83 Da from positions 23 - 59. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ427745 Genomic DNA. Translation: CAD20744.1.
    AJ427743 mRNA. Translation: CAD20742.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ427745 Genomic DNA. Translation: CAD20744.1 .
    AJ427743 mRNA. Translation: CAD20742.1 .

    3D structure databases

    ProteinModelPortali Q867F4.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.30.10. 1 hit.
    InterProi IPR003614. Scorpion_toxin-like.
    IPR001947. Scorpion_toxinS_K_inh.
    [Graphical view ]
    Pfami PF00451. Toxin_2. 1 hit.
    [Graphical view ]
    ProDomi PD003586. Scorpion_toxinS. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF57095. SSF57095. 1 hit.
    PROSITEi PS01138. SCORP_SHORT_TOXIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterisation of the genes encoding Aa1 isoforms from the scorpion Androctonus australis."
      Legros C., Bougis P.E., Martin-Eauclaire M.-F.
      Toxicon 41:115-119(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Tissue: Muscle and Venom gland.
    2. "Fast K(+) currents from cerebellum granular cells are completely blocked by a peptide purified from Androctonus australis Garzoni scorpion venom."
      Pisciotta M., Coronas F.I., Bloch C. Jr., Prestipino G., Possani L.D.
      Biochim. Biophys. Acta 1468:203-212(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 23-59, MASS SPECTROMETRY, PYROGLUTAMATE FORMATION AT GLN-23, FUNCTION.
      Strain: Garzoni.
      Tissue: Venom.
    3. "Expanding the scorpion toxin alpha-KTX 15 family with AmmTX3 from Androctonus mauretanicus."
      Vacher H., Alami M., Crest M., Possani L.D., Bougis P.E., Martin-Eauclaire M.-F.
      Eur. J. Biochem. 269:6037-6041(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SYNTHESIS OF 23-59, PHARMACOLOGICAL CHARACTERIZATION.

    Entry informationi

    Entry nameiKA154_ANDAU
    AccessioniPrimary (citable) accession number: Q867F4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 24, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Scorpion potassium channel toxins
      Nomenclature of scorpion potassium channel toxins and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3