Q867F4 (KA154_ANDAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Potassium channel toxin alpha-KTx 15.4 Short name=AaTX1 Short name=Toxin Aa1
Organism	Androctonus australis (Sahara scorpion)
Taxonomic identifier	6858 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Androctonus

Protein attributes

Sequence length	59 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Blocker of A-type voltage-gated potassium channels of cerebellar granular cells. The occlusion of the outer entry of the K+ conducting pore is partially reversible and affects both open and closed channels. It shares the same target in rat brain that BmTX3 (M.martensii), and AmmTX3 (A.mauretanicus). Ref.2
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 15 subfamily.
Mass spectrometry	Molecular mass is 3850.83 Da from positions 23 - 59. Determined by MALDI. Ref.2

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Ion channel impairing toxin Neurotoxin Potassium channel inhibitor Toxin
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.2
	Chain	23 – 59	37	Potassium channel toxin alpha-KTx 15.4		PRO_0000035310
Sites
	Site	49	1	Basic residue of the functional dyad By similarity
	Site	58	1	Aromatic residue of the functional dyad By similarity
Amino acid modifications
	Modified residue	23	1	Pyrrolidone carboxylic acid
	Disulfide bond	30 ↔ 50		By similarity
	Disulfide bond	35 ↔ 55		By similarity
	Disulfide bond	39 ↔ 57		By similarity
Experimental info
	Sequence conflict	24	1	I → N AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q867F4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E429B1B607A2F64E	FASTA	59	6,291
	10 20 30 40 50 MKFSSIILLT LLICSMSIFG NCQIETNKKC QGGSCASVCR RVIGVAAGKC INGRCVCYP

References

[1]	"Characterisation of the genes encoding Aa1 isoforms from the scorpion Androctonus australis." Legros C., Bougis P.E., Martin-Eauclaire M.-F. Toxicon 41:115-119(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Muscle and Venom gland.
[2]	"Fast K(+) currents from cerebellum granular cells are completely blocked by a peptide purified from Androctonus australis Garzoni scorpion venom." Pisciotta M., Coronas F.I., Bloch C. Jr., Prestipino G., Possani L.D. Biochim. Biophys. Acta 1468:203-212(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-59, MASS SPECTROMETRY, PYROGLUTAMATE FORMATION AT GLN-23, FUNCTION. Strain: Garzoni. Tissue: Venom.
[3]	"Expanding the scorpion toxin alpha-KTX 15 family with AmmTX3 from Androctonus mauretanicus." Vacher H., Alami M., Crest M., Possani L.D., Bougis P.E., Martin-Eauclaire M.-F. Eur. J. Biochem. 269:6037-6041(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SYNTHESIS OF 23-59, PHARMACOLOGICAL CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ427745 Genomic DNA. Translation: CAD20744.1. AJ427743 mRNA. Translation: CAD20742.1.
3D structure databases
ProteinModelPortal	Q867F4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.30.10. 1 hit.
InterPro	IPR003614. Scorpion_toxin-like. IPR001947. Scorpion_toxinS_K_inh. [Graphical view]
Pfam	PF00451. Toxin_2. 1 hit. [Graphical view]
ProDom	PD003586. Scorpion_toxinS. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF57095. SSF57095. 1 hit.
PROSITE	PS01138. SCORP_SHORT_TOXIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KA154_ANDAU

Accession

Primary (citable) accession number: Q867F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program