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Q867F4 (KA154_ANDAU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium channel toxin alpha-KTx 15.4

Short name=AaTX1
Short name=Toxin Aa1
OrganismAndroctonus australis (Sahara scorpion)
Taxonomic identifier6858 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeAndroctonus

Protein attributes

Sequence length59 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Blocker of A-type voltage-gated potassium channels of cerebellar granular cells. The occlusion of the outer entry of the K+ conducting pore is partially reversible and affects both open and closed channels. It shares the same target in rat brain that BmTX3 (M.martensii), and AmmTX3 (A.mauretanicus). Ref.2

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Sequence similarities

Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 15 subfamily.

Mass spectrometry

Molecular mass is 3850.83 Da from positions 23 - 59. Determined by MALDI. Ref.2

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionIon channel impairing toxin
Neurotoxin
Potassium channel impairing toxin
Toxin
   PTMDisulfide bond
Pyrrolidone carboxylic acid
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processdefense response

Inferred from electronic annotation. Source: InterPro

pathogenesis

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionion channel inhibitor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Ref.2
Chain23 – 5937Potassium channel toxin alpha-KTx 15.4
PRO_0000035310

Sites

Site491Basic residue of the functional dyad By similarity
Site581Aromatic residue of the functional dyad By similarity

Amino acid modifications

Modified residue231Pyrrolidone carboxylic acid
Disulfide bond30 ↔ 50 By similarity
Disulfide bond35 ↔ 55 By similarity
Disulfide bond39 ↔ 57 By similarity

Experimental info

Sequence conflict241I → N AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q867F4 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: E429B1B607A2F64E

FASTA596,291
        10         20         30         40         50 
MKFSSIILLT LLICSMSIFG NCQIETNKKC QGGSCASVCR RVIGVAAGKC INGRCVCYP 

« Hide

References

[1]"Characterisation of the genes encoding Aa1 isoforms from the scorpion Androctonus australis."
Legros C., Bougis P.E., Martin-Eauclaire M.-F.
Toxicon 41:115-119(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Muscle and Venom gland.
[2]"Fast K(+) currents from cerebellum granular cells are completely blocked by a peptide purified from Androctonus australis Garzoni scorpion venom."
Pisciotta M., Coronas F.I., Bloch C. Jr., Prestipino G., Possani L.D.
Biochim. Biophys. Acta 1468:203-212(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 23-59, MASS SPECTROMETRY, PYROGLUTAMATE FORMATION AT GLN-23, FUNCTION.
Strain: Garzoni.
Tissue: Venom.
[3]"Expanding the scorpion toxin alpha-KTX 15 family with AmmTX3 from Androctonus mauretanicus."
Vacher H., Alami M., Crest M., Possani L.D., Bougis P.E., Martin-Eauclaire M.-F.
Eur. J. Biochem. 269:6037-6041(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SYNTHESIS OF 23-59, PHARMACOLOGICAL CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ427745 Genomic DNA. Translation: CAD20744.1.
AJ427743 mRNA. Translation: CAD20742.1.

3D structure databases

ProteinModelPortalQ867F4.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.30.10. 1 hit.
InterProIPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
[Graphical view]
PfamPF00451. Toxin_2. 1 hit.
[Graphical view]
ProDomPD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF57095. SSF57095. 1 hit.
PROSITEPS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKA154_ANDAU
AccessionPrimary (citable) accession number: Q867F4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: June 1, 2003
Last modified: October 16, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Scorpion potassium channel toxins

Nomenclature of scorpion potassium channel toxins and list of entries