Reviewed, UniProtKB/Swiss-Prot Q867B0 (ELA1_CANFA)

Names and origin

Protein names

Recommended name:
    Elastase-1
    EC=3.4.21.36

Gene names

Name:	ELA1
Synonyms:	ELS1

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts upon elastin By similarity.
Catalytic activity	Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 8	8	By similarity
	Propeptide	9 – 18	10	Activation peptide		PRO_0000027673
	Chain	19 – 258	240	Elastase-1		PRO_0000027674
Regions
	Domain	19 – 256	238	Peptidase S1
Sites
	Active site	63	1	Charge relay system By similarity
	Active site	111	1	Charge relay system By similarity
	Active site	206	1	Charge relay system By similarity
	Metal binding	77	1	Calcium By similarity
	Metal binding	82	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	87	1	Calcium By similarity
Amino acid modifications
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	233	1	N-linked (GlcNAc...) Potential
	Disulfide bond	48 ↔ 64		By similarity
	Disulfide bond	145 ↔ 212		By similarity
	Disulfide bond	176 ↔ 192		By similarity
	Disulfide bond	202 ↔ 232		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q867B0-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: EA7FF7CB67F1C74F	FASTA	258	27,900
	10 20 30 40 50 60 MLVLYGHSTQ DVPETNARVV GGTEARRNSW PSQISLQYLS GGKWYHTCGG TLIRQNWVMT 70 80 90 100 110 120 AAHCVDRTMT FRVVIGEHNL SQNDGTEQSA SVQKIVVHPY WNSNDVSAGY DIALLRLAQK 130 140 150 160 170 180 VTLNSYVQLG VLPQEGAILA NNSPCYITGW GLTKTNGQLA QVLQQAYLPT VDYAICSSSS 190 200 210 220 230 240 YWGSIVKKSM VCAGGDGIRS GCQGDSGGPL HCSVNGKYTV HGVTSFVSSL GCNVSRKPTV 250 FTRVSAYITW INNVIASN

References

[1]	"Canis familiaris pancreatic elastase 1 mRNA, complete cds." Kano R., Hasegawa A. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB100595 mRNA. Translation: BAC55894.1.
RefSeq	NP_001003007.1.
UniGene	Cfa.12
3D structure databases
HSSP	HSSP built from PDB template 1QNJ based on UniProtKB P00772.
SMR	Q867B0. Positions 19-258.
ModBase	Search...
Protein family/group databases
MEROPS	S01.153.
Genome annotation databases
Ensembl	ENSCAFG00000007774. Canis familiaris. [Contig view]
GeneID	403515.
KEGG	cfa:403515.
Phylogenomic databases
HOVERGEN	Q867B0.
Enzyme and pathway databases
BRENDA	3.4.21.36. 463.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ELA1_CANFA

Accession

Primary (citable) accession number: Q867B0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)