Reviewed, UniProtKB/Swiss-Prot Q866Z5 (GCNT3_BOSMU)

Names and origin

Protein names

Recommended name:
    Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
    EC=2.4.1.102
    EC=2.4.1.150
Alternative name(s):
    C2GnT-mucin type
      Short name=C2GnT-M

Gene names

Name:

GCNT3

Organism

Bos mutus grunniens (Wild yak) (Bos grunniens)

Taxonomic identifier

30521 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R. UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the glycosyltransferase 14 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3		PRO_0000288542
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 30	18	Signal-anchor for type II membrane Potential
	Topological domain	31 – 440	410	Lumenal Potential
Amino acid modifications
	Glycosylation	108	1	N-linked (GlcNAc...) Potential
	Disulfide bond	73 ↔ 230		By similarity
	Disulfide bond	164 ↔ 384		By similarity
	Disulfide bond	185 ↔ 212		By similarity
	Disulfide bond	393 ↔ 425		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q866Z5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: EF7A85AE17A5A289	FASTA	440	50,909
	10 20 30 40 50 60 MKMTGWKKKL CRGHHLWALG CYSLLAVVAL RLSLRLKCDV DSLDLESRDF QSQRCRDVLY 70 80 90 100 110 120 KNLKLPAKRS INCSGITRGD QEAVVQALLD NLEVKKKRLP FTDTYYLNIT RDCEQFKAQR 130 140 150 160 170 180 KFIQFPLSKE ELDFPIAYSM VVHEKIENFE RLLRAVYAPQ NIYCVHVDVK SPETFKEAVK 190 200 210 220 230 240 AIISCFPNVF MASKLVPVVY ASWSRVQADL NCMEDLLQSS VPWKYLLNTC GTDFPIKTNA 250 260 270 280 290 300 EMVLALKMLN GKNSMESEIP SEYKKNRWKY RYEVTDRLYL TSKMKDPPPD NLPMFTGNAY 310 320 330 340 350 360 FVASRAFVQH VLENPKSQRL IEWVKDTYSP DEHLWATLQR APWMPGSVPY HPKYHISDMT 370 380 390 400 410 420 AIARLVKWQG HEGDVSMGAP YAPCSGIHQR AICIYGAGDL HWILQNHHLL ANKFDPRVDD 430 440 NVLQCLEEYL RHKAIYGTEL

References

[1]

"The core 2 beta-1,6-N-acetylglucosaminyltransferase-mucin encoded by bovine herpesvirus 4 was acquired from an ancestor of the African buffalo." Markine-Goriaynoff N., Georgin J.-P., Goltz M., Zimmermann W., Broll H., Wamwayi H.M., Pastoret P.-P., Sharp P.M., Vanderplasschen A. J. Virol. 77:1784-1792(2003) [PubMed: 12525612] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF465336 Genomic DNA. Translation: AAO22163.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT14. Glycosyltransferase Family 14.
Phylogenomic databases
HOVERGEN	Q866Z5.
Family and domain databases
InterPro	IPR003406. Glyco_trans_14. [Graphical view]
PANTHER	PTHR19297. Glyco_trans_14. 1 hit.
Pfam	PF02485. Branch. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCNT3_BOSMU

Accession

Primary (citable) accession number: Q866Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	June 1, 2003
Last modified:	April 14, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)