Q866Z4 (GCNT3_SYNCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
EC=2.4.1.102
EC=2.4.1.150

Alternative name(s):
C2GnT-mucin type
Short name=C2GnT-M

Gene names

Name:

GCNT3

Organism

Syncerus caffer (Cape buffalo)

Taxonomic identifier

9970 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Syncerus

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R. UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the glycosyltransferase 14 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3		PRO_0000288549
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 30	18	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	31 – 440	410	Lumenal Potential
Amino acid modifications
	Glycosylation	108	1	N-linked (GlcNAc...) Potential
	Disulfide bond	73 ↔ 230		By similarity
	Disulfide bond	164 ↔ 384		By similarity
	Disulfide bond	185 ↔ 212		By similarity
	Disulfide bond	393 ↔ 425		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q866Z4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 480A0D5847B5843B	FASTA	440	51,079
	10 20 30 40 50 60 MKMTGWKKKL CRGHHLWALG CYMLLAVVAL RLSLRLKCDV DSLDLESRDF QSQRCRDILY 70 80 90 100 110 120 KNLKLPAKRS INCSGITRGD QEAVVQALLD NLEVKKKRLP FTDTYYLNIT RDCERFKAQR 130 140 150 160 170 180 KFIQFPLSKE ELDFPIAYSM VVHEKIENFE RLLRAVYAPQ NIYCVHVDVK SPETFKEAVK 190 200 210 220 230 240 AIISCFPNVF MASKLVPVVY ASWSRVQADL NCMEDLLQSS VPWKYLLNTC GTDFPIKTNA 250 260 270 280 290 300 EMVLALKMLN GKNSMESEIP SEYKKNRWKY RYEVTDRLYL TSKMKDPPPD NLPMFTGNAY 310 320 330 340 350 360 FVASRAFVQH VLENPKSQRL IEWVKDTYSP DEHLWATLQR APWMPGSVPY HPKYHISDMT 370 380 390 400 410 420 AIARLVKWQD HEGDVSMGAP YAPCSGIHQR AICIYGAGDL YWILQNHHLL ANKFDPRVDD 430 440 NVLQCLEEYL RHKAIYGTEL

References

[1]

"The core 2 beta-1,6-N-acetylglucosaminyltransferase-mucin encoded by bovine herpesvirus 4 was acquired from an ancestor of the African buffalo." Markine-Goriaynoff N., Georgin J.-P., Goltz M., Zimmermann W., Broll H., Wamwayi H.M., Pastoret P.-P., Sharp P.M., Vanderplasschen A. J. Virol. 77:1784-1792(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

[2]

"Phylogeographical analysis of bovine herpesvirus 4: demonstration that inter-strain recombination events took place after acquisition of the Bo17 gene from an ancestor of the African buffalo." Dewals B., Markine-Goriaynoff N., de Fays K., Minner F., Daix V., Vercammen F., Gaillard C., Sharp P.M., Vanderplasschen A. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF465337 Genomic DNA. Translation: AAO22164.1. AY847314 Genomic DNA. Translation: AAY21833.1. AY847315 Genomic DNA. Translation: AAY21834.1. AY847316 Genomic DNA. Translation: AAY21835.1. AY847317 Genomic DNA. Translation: AAY21836.1. AY847318 Genomic DNA. Translation: AAY21837.1. AY847319 Genomic DNA. Translation: AAY21838.1. AY847320 Genomic DNA. Translation: AAY21839.1.
3D structure databases
ProteinModelPortal	Q866Z4.
SMR	Q866Z4. Positions 70-435.
ModBase	Search...
Protein family/group databases
CAZy	GT14. Glycosyltransferase Family 14.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG051711.
Enzyme and pathway databases
UniPathway	UPA00378.
Family and domain databases
InterPro	IPR003406. Glyco_trans_14. [Graphical view]
Pfam	PF02485. Branch. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCNT3_SYNCA

Accession

Primary (citable) accession number: Q866Z4
Secondary accession number(s): Q1M0V7, Q1M0V9, Q1M0W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	June 1, 2003
Last modified:	April 3, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program