Reviewed, UniProtKB/Swiss-Prot Q866F4 (ADCYA_RABIT)

Names and origin

Protein names

Recommended name:
    Adenylate cyclase type 10
    EC=4.6.1.1
Alternative name(s):
    Testicular soluble adenylyl cyclase
    Germ cell soluble adenylyl cyclase
      Short name=sAC

Gene names

Name:	ADCY10
Synonyms:	SAC

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	1610 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor By similarity.
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Enzyme regulation	Activated by manganese or magnesium ions. In the presence of magnesium ions, the enzyme is activated by bicarbonate while in the presence of manganese ions, the enzyme is inhibited by bicarbonate. In the absence of magnesium and bicarbonate, the enzyme is weakly activated by calcium By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm › cytoskeleton. Cytoplasm › perinuclear region. Note: Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane-associated protein, protein concentrated in the perinuclear region and protein colocalized with actin or tubulin By similarity.
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 guanylate cyclase domains.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cAMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW intracellular signaling cascade Inferred from electronic annotation. Source: InterPro spermatogenesis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1610	1610	Adenylate cyclase type 10		PRO_0000317103
Regions
	Domain	42 – 179	138	Guanylate cyclase 1
	Domain	293 – 418	126	Guanylate cyclase 2
	Compositional bias	909 – 912	4	Poly-Glu
Sites
	Metal binding	47	1	Magnesium 1 By similarity
	Metal binding	47	1	Magnesium 2 By similarity
	Metal binding	48	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	99	1	Magnesium 1 By similarity
	Metal binding	99	1	Magnesium 2 By similarity
Amino acid modifications
	Modified residue	810	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q866F4-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: B86C2B4E8A4E3207	FASTA	1,610	185,520
	10 20 30 40 50 60 MNTRKEELQD RAIVRIAAHL PDLIVYGDFS PQRPSVDYFD GVLMFVDISG FTAMTEKFST 70 80 90 100 110 120 AMYMDRGAEQ LVEILNYYIS AIVEKVLIFG GDILKFAGDA LLALWKVERK QLKNIITVVI 130 140 150 160 170 180 KCSLEIHGLF GTQESEEGLD IRVKIGLAAG HISMLVFGDE TRNHFLVIGQ AVDDVRLAQN 190 200 210 220 230 240 MARMNDVILS PNCWQLCDRS MIEIERIPDQ RAVKVNFLKP PPSFNFDEFF NKCMTFMDYY 250 260 270 280 290 300 PSGDHKNLLR LACMLESDPD LELSLQKYVM ESILKQIDDK QLRGYLSELR PVTIVFVNLM 310 320 330 340 350 360 FQDQNKAEVI GSAIQDACVH ISSVLKVFRG QINKVFMFDK GCSFLCVFGF PGEKAPDEVT 370 380 390 400 410 420 HALESAVDIF DFCSQVHKIH TVSIGVASGI VFCGIVGHTV RHEYTVIGQK VNIAARMMMY 430 440 450 460 470 480 YPGIVTCDSV TYNGSNLPPY FFKELPKKLM KGVGDSGPVY QCLGLNEKVM FGMAYLTCNR 490 500 510 520 530 540 NEGYPLLGRD KEIKYFMCTM KEFLMSNCSR VLMYEGLSGF GKSRILMEIE YLAQGENHRT 550 560 570 580 590 600 IAIALTKVSF HQNFYTIQIL MANVLGLDTC KHYKERQTNL QNKVKTLLDE KFHCLLNDIF 610 620 630 640 650 660 HVQFPISREI SKMSTFRKQK QLEALFMKIL EQTVKEERII FIIDEAQFVD YASWIFMEKL 670 680 690 700 710 720 IRTVPIFIIM SLSPFTEIPC AAASAIMKNR NTTYVTLGAV QPNDIRNKVC LDLNVSSIPK 730 740 750 760 770 780 ELDLYLVEGS CGIPFYCEEL VKNLDHHRVL VFQQMETEEK TKVTWNNLFK NFIKPTEEFK 790 800 810 820 830 840 MSGLGNEEGT EEICKLASGV RLKNLSPPAS LKEISLVQLD SMSLSHQMLV RCAAIIGLTF 850 860 870 880 890 900 TTELLFEILP CWNMKMMIKA LATLVESNIF DCFRDGKDLR LALKQNAASF EVHNRSLSLQ 910 920 930 940 950 960 PTEGIAHGEE EELRELESEV IECHIIRFCK PMMQKTAYEL WLKDQKKAMH LKCARFLEEN 970 980 990 1000 1010 1020 AHRCDHCRSG DFIPYHHFTV DIRLNTLDMD TIKKMATSHG FETEEEIKIS RAGIPKNSEL 1030 1040 1050 1060 1070 1080 FSENLSPEEI GERILGFFDV ILTKMKTSKE DIIPLESCQC EEILEIVILP LAQHFLALGE 1090 1100 1110 1120 1130 1140 NNKALYYFLE ITSAYLTLGD NYMAYMYLNE GERLLKTLKK EKSWSQTFES ATFYSLKGQV 1150 1160 1170 1180 1190 1200 CFNMGQMVLA KKMLRKALKL LNRIFPYNLI SLFLHTHMEK NRHFHYVTQQ AQESSPPGKK 1210 1220 1230 1240 1250 1260 RLAHLYQQTA CFSLLWQIYS LNYFFHHKYY GHLAAMMELN TALETQNDFQ IIKAYLDYAM 1270 1280 1290 1300 1310 1320 YHHLAGYQGV WFKYEVKAME QIFNLPLKGE GIEIVAYVAG KLSYIKLMMG YLDLAIELGA 1330 1340 1350 1360 1370 1380 RAHKMWALLQ NPNQHYVVLC RLSKSLFLKN RYKHLIQMLR RLWDLSVAEG HIISKAFFYL 1390 1400 1410 1420 1430 1440 VCLDIMLYSG FVYRTFEECL EFIIQNEDNR ILKFQSGLLL GLYSSIAIWY GRLQEWDNFY 1450 1460 1470 1480 1490 1500 VFSNRAKTLV SRRTPTILYY DGVSRYMEGQ VLQLQKQIEE QSETAQDSGV ELLKSLESLV 1510 1520 1530 1540 1550 1560 AQNTTGPVFY PRLYHLMAYI CILMGDGQNC DLFLNTALKL SEIQGNVLEK CWLNMSKEWW 1570 1580 1590 1600 1610 YSNCTLTEDQ WLHTILSLPA WEKIVSGKVN IHDVQKNKFL MRVNILDNPF

References

[1]	"Conservation of functional domain structure in bicarbonate-regulated 'soluble' adenylyl cyclases in bacteria and eukaryotes." Kobayashi M., Buck J., Levin L.R. Dev. Genes Evol. 214:503-509(2004) [PubMed: 15322879] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY212921 mRNA. Translation: AAO38673.1.
RefSeq	NP_001075622.1.
UniGene	Ocu.3193
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	100008902.
Organism-specific databases
CTD	100008902.
Phylogenomic databases
HOVERGEN	Q866F4.
Enzyme and pathway databases
BRENDA	4.6.1.1. 255.
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR018297. A/G_cyclase_CS. IPR016577. Adenylate_cylcase_typ10. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 2 hits.
Pfam	PF00211. Guanylate_cyc. 2 hits. [Graphical view]
PIRSF	PIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
SMART	SM00044. CYCc. 1 hit. [Graphical view]
PROSITE	PS00452. GUANYLATE_CYCLASE_1. False negative. PS50125. GUANYLATE_CYCLASE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADCYA_RABIT

Accession

Primary (citable) accession number: Q866F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 1, 2003
Last modified:	October 13, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)