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Protein

Pro-vaccinia growth factor

Gene

VGF

Organism
Vaccinia virus (strain L-IVP) (VACV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Vaccinia growth factor stimulates cellular proliferation (hyperplasia) around infected cells. This effect is beneficial for virus replication in vivo, because poxviruses replicate possibly better in proliferating cells than in quiescent cells. Acts by binding host EGFR, inducing its dimerization, autophosphorylation and leading to activation of several cellular pathways regulating cell proliferation or cell survival. The activation by host EFGR of mitogen activated protein kinases (MAPK) and extracellular-signal regulated kinases (ERK) are essential for the positive effect of vaccinia growth factor on poxvirus virulence in vivo (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Growth factor

Keywords - Biological processi

Host-virus interaction

Names & Taxonomyi

Protein namesi
Recommended name:
Pro-vaccinia growth factor
Short name:
Pro-VGF
Cleaved into the following chain:
Vaccinia growth factor
Short name:
VGF
Alternative name(s):
Secreted epidermal growth factor-like
Gene namesi
Name:VGF
OrganismiVaccinia virus (strain L-IVP) (VACV)
Taxonomic identifieri31531 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

Pro-vaccinia growth factor :
  • Host membrane By similarity; Single-pass type I membrane protein By similarity
Vaccinia growth factor :
  • Secreted By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 10082ExtracellularSequence analysisAdd
BLAST
Transmembranei101 – 12121HelicalSequence analysisAdd
BLAST
Topological domaini122 – 14019CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 140122Pro-vaccinia growth factorPRO_0000007597Add
BLAST
Chaini19 – 9678Vaccinia growth factorSequence analysisPRO_0000412916Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi34 – 341N-linked (GlcNAc...); by hostSequence analysis
Disulfide bondi45 ↔ 58PROSITE-ProRule annotation
Disulfide bondi53 ↔ 69PROSITE-ProRule annotation
Disulfide bondi71 ↔ 80PROSITE-ProRule annotation
Glycosylationi95 – 951N-linked (GlcNAc...); by hostSequence analysis

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei96 – 972Cleavage (By host protease)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Vaccinia growth factor interacts with host EGFR and promotes EGFR dimerization.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ86607.
SMRiQ86607. Positions 41-85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 8141EGF-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 EGF-like domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR011170. GF_C11R.
[Graphical view]
PIRSFiPIRSF001779. GF_C11R. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q86607-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMKYLMLLF AAMIIRSFAD SGNAIETTLP EITNATTDIP AIRLCGPEGD
60 70 80 90 100
GYCLHGDCIH ARDIDGMYCR CSHGYTGIRC QHVVLVDYQR SENPNTTTSY
110 120 130 140
IPSPGIVLVL VGIIIITCCS LSVYRFTRRT KLPIQDMVVP
Length:140
Mass (Da):15,492
Last modified:November 1, 1996 - v1
Checksum:iD80F4E7FF9187D26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S61049 Genomic DNA. Translation: AAB26161.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S61049 Genomic DNA. Translation: AAB26161.1.

3D structure databases

ProteinModelPortaliQ86607.
SMRiQ86607. Positions 41-85.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR011170. GF_C11R.
[Graphical view]
PIRSFiPIRSF001779. GF_C11R. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVGF_VACCP
AccessioniPrimary (citable) accession number: Q86607
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.