Reviewed, UniProtKB/Swiss-Prot Q865R1 (PAOX_BOVIN)

Names and origin

Protein names

Recommended name:
    Peroxisomal N(1)-acetyl-spermine/spermidine oxidase
    EC=1.5.3.11
Alternative name(s):
    Polyamine oxidase

Gene names

Name:	PAOX
Synonyms:	PAO

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Flavoenzyme which catalyzes the oxidation of N(1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N(1)-acetylspermidine to putrescine. Substrate specificity: N(1)-acetylspermine = N(1)-acetylspermidine > N(1),N(12)-diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration.
Catalytic activity	N(1)-acetylspermine + O(2) + H(2)O = N(1)-acetylspermidine + 3-aminopropanal + H(2)O(2).
Cofactor	FAD By similarity.
Pathway	Amine and polyamine metabolism; spermine metabolism.
Subunit structure	Monomer By similarity.
Subcellular location	PeroxisomeBy similarity. CytoplasmBy similarity.
Miscellaneous	Oxidizes N(1)-acetylated polyamines on the exo-side of their N(4)-amino groups. Plant PAO oxidizes spermine on the endo-side of the N(4)-nitrogen By similarity.
Sequence similarities	Belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
Cellular component	Cytoplasm Peroxisome
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro polyamine oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 6	6			PRO_0000236802
	Chain	7 – 512	506	Peroxisomal N(1)-acetyl-spermine/spermidine oxidase		PRO_0000099874
Regions
	Motif	510 – 512	3	Microbody targeting signal Potential
Experimental info
	Sequence conflict	7	1	Q → E AA sequence Ref.2
	Sequence conflict	482	1	T → P in AAN40707. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q865R1-1 [UniParc]. Last modified May 30, 2006. Version 3. Checksum: 6164E3A77841F36F	FASTA	512	56,325
	10 20 30 40 50 60 MQSGGRQAEA PGRGPRVLVV GGGIAGLGAA QRLCRHPAFS HLRVLEATAR AGGRIRSEHS 70 80 90 100 110 120 FGGVVEVGAH WIHGPSQGNP VFQLAAKYGL LGEKALSEEN QLIETGGHVG LPSVSYASSG 130 140 150 160 170 180 VSVSLELVAE MASLFYSLID QTREFLQAAE TTPPSVGEYL KEKIRQHMAG WTEDEETKKL 190 200 210 220 230 240 KLAILKNLFN VECCVSGTHS MDLVALAPFG EYTVLPGLDC TFPEGYQGLT DCIMASLPKD 250 260 270 280 290 300 VMVFDKPVKT IHWNGSFREA SAPGETFPVL VECEDGDCFP AHHVVVTVPL GFFKKHLDTF 310 320 330 340 350 360 FEPPLPTEKV EAIRKIGFGT NNKIFLEFEE PFWEPDCQHI QVVWEDMSPL EDTAPELQDA 370 380 390 400 410 420 WFKKLIGFWV LPPFQASHVL CGFIAGLESE FMETLSDEDV LRSLTQVLRR VTGNPQLPAP 430 440 450 460 470 480 RSMLRSCWHS APYTRGSYSY VAVGSSGDDM DRLAQPLPSD GKGAQLQVLF AGEATHRTFY 490 500 510 STTHGALLSG WREADRLMTL WDPQAQWPEP RL

References

[1]	"Comparative mapping of the bovine SPRN locus." Ferretti L., Uboldi C., Del Vecchio I., Eggen A., Brunner R., Iannuzzi L. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"Cloning, sequencing, and heterologous expression of the murine peroxisomal flavoprotein, N(1)-acetylated polyamine oxidase." Wu T., Yankovskaya V., McIntire W.S. J. Biol. Chem. 278:20514-20525(2003) [PubMed: 12660232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 62-512, PROTEIN SEQUENCE OF 7-27; 57-76 AND 497-511, CHARACTERIZATION. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ058607 mRNA. Translation: AAY83881.1. DQ058602 Genomic DNA. Translation: AAY83877.1. AF226658 mRNA. Translation: AAN40707.1.
RefSeq	NP_001013620.2.
UniGene	Bt.36568
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000018321. Bos taurus. [Contig view]
GeneID	282639.
KEGG	bta:282639.
Phylogenomic databases
HOVERGEN	Q865R1.
Family and domain databases
InterPro	IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PAOX_BOVIN

Accession

Primary (citable) accession number: Q865R1
Secondary accession number(s): Q4PS79

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	May 30, 2006
Last modified:	November 25, 2008
This is version 35 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)