Q86567 (MREP_BBTVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Master replication protein
Short name=M-Rep
EC=2.7.7.-
EC=3.1.21.-
EC=3.6.1.3

Gene names

Name:	DNA-R
Synonyms:	C1

Organism

Banana bunchy top virus (isolate Autralia) (BBTV) [Reference proteome]

Taxonomic identifier

645099 [NCBI]

Taxonomic lineage

Viruses › ssDNA viruses › Nanoviridae › Babuvirus › Banana bunchy top virus

Virus host

Musa [TaxID: 4640]

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential for the replication of all genomic viral ssDNA (trans-replication). The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-A[GT]TATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Cofactor	Divalent metal cations, possibly magnesium or manganese By similarity.
Subunit structure	Homooligomer Potential. Rep binds to repeated DNA motifs (iterons) By similarity.
Subcellular location	Host nucleus Potential.
Domain	There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR By similarity.
Sequence similarities	Belongs to the nanoviridea/circoviridae replication-associated protein family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Host nucleus
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding
Molecular function	Endonuclease Helicase Hydrolase Nuclease Nucleotidyltransferase Transferase
PTM	Covalent protein-DNA linkage
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC DNA replication Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC protein-DNA covalent cross-linking Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, uncoupled Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA helicase activity Inferred from electronic annotation. Source: InterPro endodeoxyribonuclease activity, producing 5'-phosphomonoesters Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotidyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Master replication protein		PRO_0000379898
Regions
	Nucleotide binding	180 – 188	9	ATP By similarity
	Motif	10 – 13	4	RCR-1 By similarity
	Motif	41 – 46	6	RCR-2 By similarity
	Motif	50 – 70	21	Nuclear localization signal Potential
	Motif	79 – 82	4	RCR-3 By similarity
	Motif	96 – 102	7	Nuclear localization signal Potential
Sites
	Active site	79	1	For DNA cleavage activity By similarity
	Metal binding	33	1	Divalent metal cation Potential
	Metal binding	41	1	Divalent metal cation Potential
	Metal binding	84	1	Divalent metal cation Potential

Sequences

	Sequence		Length	Mass (Da)
	Q86567 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2508FC37F1AEB013	FASTA	286	33,604
	10 20 30 40 50 60 MARYVVCWMF TINNPTTLPV MRDEIKYMVY QVERGQEGTR HVQGYVEMKR RSSLKQMRGF 70 80 90 100 110 120 FPGAHLEKRK GSQEEARSYC MKEDTRIEGP FEFGSFKLSC NDNLFDVIQD MRETHKRPLE 130 140 150 160 170 180 YLYDCPNTFD RSKDTLYRVQ AEMNKTKAMN SWRTSFSAWT SEVENIMAQP CHRRIIWVYG 190 200 210 220 230 240 PNGGEGKTTY AKHLMKTRNA FYSPGGKSLD ICRLYNYEDI VIFDIPRCKE DYLNYGLLEE 250 260 270 280 FKNGIIQSGK YEPVLKIVEY VEVIVMANFL PKEGIFSEDR IKLVSC

References

[1]	"Nucleotide sequence of one component of the banana bunchy top virus genome contains a putative replicase gene." Harding R.M., Burns T.M., Hafner G.J., Dietzgen R.G., Dale J.L. J. Gen. Virol. 74:323-328(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	S56276 Genomic DNA. Translation: AAB25544.1.
PIR	JQ1960.
RefSeq	NP_604483.1. NC_003479.1.
3D structure databases
ProteinModelPortal	Q86567.
SMR	Q86567. Positions 2-95.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	963871.
Family and domain databases
InterPro	IPR000605. Helicase_SF3_ssDNA/RNA_vir. IPR003365. Viral_rep_N. [Graphical view]
Pfam	PF00910. RNA_helicase. 1 hit. PF02407. Viral_Rep. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MREP_BBTVA

Accession

Primary (citable) accession number: Q86567

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program