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Q86567 (MREP_BBTVA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Master replication protein

Short name=M-Rep
EC=2.7.7.-
EC=3.1.21.-
EC=3.6.1.3
Gene names
Name:DNA-R
Synonyms:C1
OrganismBanana bunchy top virus (isolate Autralia) (BBTV) [Reference proteome]
Taxonomic identifier645099 [NCBI]
Taxonomic lineageVirusesssDNA virusesNanoviridaeBabuvirus
Virus hostMusa [TaxID: 4640]

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Essential for the replication of all genomic viral ssDNA (trans-replication). The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-A[GT]TATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Cofactor

Divalent metal cations, possibly magnesium or manganese By similarity.

Subunit structure

Homooligomer Potential. Rep binds to repeated DNA motifs (iterons) By similarity.

Subcellular location

Host nucleus Potential.

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR By similarity.

Sequence similarities

Belongs to the nanoviridea/circoviridae replication-associated protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 286286Master replication protein
PRO_0000379898

Regions

Nucleotide binding180 – 1889ATP By similarity
Motif10 – 134RCR-1 By similarity
Motif41 – 466RCR-2 By similarity
Motif50 – 7021Nuclear localization signal Potential
Motif79 – 824RCR-3 By similarity
Motif96 – 1027Nuclear localization signal Potential

Sites

Active site791For DNA cleavage activity By similarity
Metal binding331Divalent metal cation Potential
Metal binding411Divalent metal cation Potential
Metal binding841Divalent metal cation Potential

Sequences

Sequence LengthMass (Da)Tools
Q86567 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 2508FC37F1AEB013

FASTA28633,604
        10         20         30         40         50         60 
MARYVVCWMF TINNPTTLPV MRDEIKYMVY QVERGQEGTR HVQGYVEMKR RSSLKQMRGF 

        70         80         90        100        110        120 
FPGAHLEKRK GSQEEARSYC MKEDTRIEGP FEFGSFKLSC NDNLFDVIQD MRETHKRPLE 

       130        140        150        160        170        180 
YLYDCPNTFD RSKDTLYRVQ AEMNKTKAMN SWRTSFSAWT SEVENIMAQP CHRRIIWVYG 

       190        200        210        220        230        240 
PNGGEGKTTY AKHLMKTRNA FYSPGGKSLD ICRLYNYEDI VIFDIPRCKE DYLNYGLLEE 

       250        260        270        280 
FKNGIIQSGK YEPVLKIVEY VEVIVMANFL PKEGIFSEDR IKLVSC 

« Hide

References

[1]"Nucleotide sequence of one component of the banana bunchy top virus genome contains a putative replicase gene."
Harding R.M., Burns T.M., Hafner G.J., Dietzgen R.G., Dale J.L.
J. Gen. Virol. 74:323-328(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S56276 Genomic DNA. Translation: AAB25544.1.
PIRJQ1960.
RefSeqNP_604483.1. NC_003479.1.

3D structure databases

ProteinModelPortalQ86567.
SMRQ86567. Positions 2-95.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID963871.

Family and domain databases

InterProIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR003365. Viral_rep_N.
[Graphical view]
PfamPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMREP_BBTVA
AccessionPrimary (citable) accession number: Q86567
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families