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Q86515

- VP7_ROTRF

UniProt

Q86515 - VP7_ROTRF

Protein

Outer capsid glycoprotein VP7

Gene
N/A
Organism
Rotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved By similarity.By similarity

    GO - Biological processi

    1. viral process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction

    Keywords - Ligandi

    Calcium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid glycoprotein VP7
    OrganismiRotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A)
    Taxonomic identifieri10933 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    ProteomesiUP000007179: Genome

    Subcellular locationi

    Virion By similarity. Host endoplasmic reticulum lumen Curated
    Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum lumen Source: UniProtKB-SubCell
    2. T=13 icosahedral viral capsid Source: UniProtKB-KW
    3. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host endoplasmic reticulum, Outer capsid protein, T=13 icosahedral capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 5050Sequence AnalysisAdd
    BLAST
    Chaini51 – 326276Outer capsid glycoprotein VP7Sequence AnalysisPRO_0000369108Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi238 – 2381N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi318 – 3181N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    The N-terminus is blocked possibly by pyroglutamic acid.By similarity
    N-glycosylated.By similarity
    Intramolecular disulfide bonds.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homotrimer; in the presence of calcium By similarity. Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 By similarity.By similarity

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the rotavirus VP7 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR001963. VP7.
    [Graphical view]
    PfamiPF00434. VP7. 1 hit.
    [Graphical view]
    ProDomiPD000191. VP7. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform 1 (identifier: Q86515-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MYGIEYTTIL IFLTSITLLN YMLKSITRIM DYIIYRFLLI VVILATIIKA    50
    QNYGVNLPIT GSMDTAYADS TQSEPFLTST LCLYYPVEAS NEIADTEWKD 100
    TLSQLFLTKG WPTGSVYLKE YADIAAFSVE PQLYCDYNLV LMKYDSTQEL 150
    DMSELADLIL NEWLCNPMDI TLYYYQQTDE ANKWISTGSS CTVKVCPLNT 200
    QTLGIGCLIT NPDTFETVAT TEKLVITDVV DGVNHKLNVT TATCTIRNCK 250
    KLGPRENVAV IQVGGANILD ITADPTTTPQ TERMMRINWK KWWQVFYTVV 300
    DYVNQIIQTM SKRSRSLNSS AFYYRV 326
    Length:326
    Mass (Da):37,085
    Last modified:November 1, 1996 - v1
    Checksum:i66C66C7156889B9D
    GO
    Isoform 2 (identifier: Q86515-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-29: Missing.

    Note: Produced by alternative initiation at Met-30 of isoform 1. No experimental confirmation available.

    Show »
    Length:297
    Mass (Da):33,677
    Checksum:iE94658BAF7A0D708
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2929Missing in isoform 2. CuratedVSP_038611Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65940 mRNA. Translation: CAA46743.1.
    PIRiS25546.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65940 mRNA. Translation: CAA46743.1 .
    PIRi S25546.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001963. VP7.
    [Graphical view ]
    Pfami PF00434. VP7. 1 hit.
    [Graphical view ]
    ProDomi PD000191. VP7. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the gene encoding the outer glycoprotein of the bovine rotavirus (RF strain) and comparison with homologous genes from four bovine, simian and human rotaviruses."
      Cohen J.
      Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiVP7_ROTRF
    AccessioniPrimary (citable) accession number: Q86515
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In group A rotaviruses, VP7 defines the G serotype.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3